The aim of the experiment was to explore the feasibility of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens. 100 g/kg, 200 g/kg, 300 g/kg of discarded nutrient medium of Cordy...The aim of the experiment was to explore the feasibility of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens. 100 g/kg, 200 g/kg, 300 g/kg of discarded nutrient medium of Cordyceps militaris were added to the basal diet of laying hens. The results showed that the optimal addition of discarded nutrient medium of Cordyceps militaris in the diet of laying hens was 10%. According to the results of measuring the conventional indicators of eggs, the weight of eggs produced by laying hens fed with discarded nutrient medium of Cordyceps militaris was higher than that of laying hens fed with ordinary laying hens. The content of interleukin-1(IL-1) in the experimental group was significantly higher than that in the control group, and the concentration of IL-1 increased by 141.5 pg/mL, which indicated that the application of discarded nutrient medium of Cordyceps militaris effectively improved the immunity of laying hens. The high-throughput analysis of the intestinal contents of the two groups of laying hens showed that the microbial population abundance of the intestinal tract of the experimental group was greater than that of control group, and the application of discarded nutrient medium of Cordyceps militaris increased the diversity of bacteria in the intestinal tract of laying hens. In addition, the sensitivity of some pathogenic bacteria in the intestinal tract of chickens to drugs was also increased, thereby reducing the use of antibiotics. The secondary utilization of discarded nutrient medium of Cordyceps militaris has great development and utilization prospects, which provided a scientific reference and basic theoretical basis for the development of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens.展开更多
Five Chinese milk vetch（Astragalus sinicus） varieties were selected to make a comparison in order to screen effective varieties which could suit for the double-rice cropping system of Hunan Province. The results sho...Five Chinese milk vetch（Astragalus sinicus） varieties were selected to make a comparison in order to screen effective varieties which could suit for the double-rice cropping system of Hunan Province. The results showed that the growth period of Xiangzi 2 is shorter than that of Xiangfei 2, Xiangfei 3 and Yujiangdaye,and is similar to that of Xinyang Chinese milk vetch. The full-bloom stage of Xiangzi 2 is also similar to Xinyang variety and obviously earlier than that of the other three varieties. The fresh grass yield of Xiangzi 2 in full-bloom stage is 23 842.5kg/hm^2, which increases by 39.9% compared with Xinyang variety. Nitrogen content in the fresh grass of Xiangzi 2 is higher than that of the other four varieties. In conclusion, Xiangzi 2 is an extremely early-blossoming and early-maturing Chinese milk vetch variety with moderate fresh grass yield, high nutrient contents, stable characters and wide adaptability. So, Xiangzi 2 is suitable for planting in double-rice cropping region in Hunan Province and other same latitude regions which have similar ecological conditions.展开更多
The increasing trend of air temperature along with the climate warming has been accepted gradually by scientists and by the general public. Qinghai_Xizang Plateau, a unique geographic unit due to high_altitude climate...The increasing trend of air temperature along with the climate warming has been accepted gradually by scientists and by the general public. Qinghai_Xizang Plateau, a unique geographic unit due to high_altitude climate, is one of the most susceptible regions to climate warming. Its ecosystem is very fragile and sensitive to climate change. In order to get a better understanding of the impacts of climate warming on the nutrient contents of herbage grown in Qinghai_Xizang Plateau, a simulative study was implemented at Daban Moutain by using temperature differences resulted from sites selected at different altitudes and nutrient contents and in vitro digestibility were determined for assessing the quality of the grown herbage. There were significant downtrends in crude protein (CP), ether extract (EE) and nitrogen free extract (NFE) contents of herbage along with the increase of temperature. It had a positive correlation between temperature and content of acid detergent fibre (ADF), acid detergent lignin (ADL) in herbage. In vitro digestibility of herbage decreased along with the increase of temperature. The results of this study indicated that climate warming significantly influence nutrient contents and in vitro digestibility of herbage grown in Qinghai_Xizang Plateau. It is suggested that the future climate warming especially the gradual rise of the night temperature could cause negative effect on herbage quality grown in Qinghai_Xizang Plateau by decreasing CP, EE, and NFE contents and increasing some indigestible ingredients such as crude fibre (CF), neutral detergent fibre (NDF), ADF, and ADL. This, consequently, decreases the ruminant assimilation ability.展开更多
[Objective] The study had developed a means of rapid propagation Pteris vittata L.by tissue culture. The species was a perennial fern belonging to the genus Pteris. [Metbed] The leaf bud of P. vittata collected in fie...[Objective] The study had developed a means of rapid propagation Pteris vittata L.by tissue culture. The species was a perennial fern belonging to the genus Pteris. [Metbed] The leaf bud of P. vittata collected in field conditions as explantsand the 1/2 MS ＋ 3% sucrose ＋ 0.7% agar as the basic medium were used to screen the medium formula of the phytohormone ratio for callus induction and subculture of P. vittata. [Result] The best medium formula for each step was list below： 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.1 mg/L KT ＋ 0.5 mg/L 2, 4-D for in- ducing the callus from explants; 1/2MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 1.0 mg/L KT ＋ 0.01 mg/L 2,4-D for inducing the GGB from callus and the seedlings from GGB. In addition, 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.5 mg/L 2,4-D for the subculture could make the continued proliferation of callus. [Cen- clusioa] This study makes an applicable procedure by the direct use of field materi- als, for propagating P. vittata in a simplified and rapid mode.展开更多
The line of T240-6 was selected from 32 SC 2 lines of immature embryo culture of T240 (common wheat (Triticum aestivum L.)× Wheat-Haynaldia villosa (L.) Schur. 6D/6V substitution line) through powdery mildew ch...The line of T240-6 was selected from 32 SC 2 lines of immature embryo culture of T240 (common wheat (Triticum aestivum L.)× Wheat-Haynaldia villosa (L.) Schur. 6D/6V substitution line) through powdery mildew characterization, glutamate oxaloacetate transaminase (GOT) enzyme and gliadin (Gli) analyses and in situ hybridization. All of the individual plants resistant to powdery mildew lacked the locus of GOT at 6VL arm (GOT-V 2) and had gliadin locus at 6VS arm (Gli-V 2) of Haynaldia villosa. All the plants resistant to powdery mildew had one or two telocentric chromosomes that did not pair with wheat chromosomes but paired between themselves. T240-6 was identified as a telocentric line through in situ hybridization.展开更多
Seasonal dynamics of above- and belowground biomass and nutrient characteristics （nitrogen, carbon, and phosphorus） of Carex lasiocarpa were investigated in the typical wetland of Sanjiang Plain, China from May 2007...Seasonal dynamics of above- and belowground biomass and nutrient characteristics （nitrogen, carbon, and phosphorus） of Carex lasiocarpa were investigated in the typical wetland of Sanjiang Plain, China from May 2007 to September 2008. The results show that the changes of aboveground biomass during the growing season are best described by the twice function curve model, whereas the changes of belowground biomass follow the exponential increase curve model. Both the organic carbon contents in the above- and belowground plant parts show significant positive linear correlations with the growing time, and the coefficients R2 are 0.983 and 0.746, respectively. The carbon accu-mulations of the above- and belowground plant parts during the growing season show the same dynamics as those of the biomass. However, the nitrogen contents and accumulation in C. lasiocarpa aboveground and belowground parts show exponential increase during the growing season. The dynamics of C. lasiocarpa phosphorus contents follows the twice function curve model, whereas the accumulation of phosphorus shows the linear increase. The ratios of C/N in different parts of C. lasiocarpa fit the negative linear relations with total nitrogen content in the growing season. Moreover, the ratios of C/P in C. lasiocarpa plant also fit the negative linear relations with total phosphorus content. The results show that nitrogen is the primary limiting nutrient for C. lasiocarpa growth as compared with carbon and phosphorus.展开更多
基金Project supported by Key Project of Education Department of Liaoning Province(LJKZZ20220116)Surface Project of Science and Technology Department of Liaoning Province(2023-MS-251).
文摘The aim of the experiment was to explore the feasibility of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens. 100 g/kg, 200 g/kg, 300 g/kg of discarded nutrient medium of Cordyceps militaris were added to the basal diet of laying hens. The results showed that the optimal addition of discarded nutrient medium of Cordyceps militaris in the diet of laying hens was 10%. According to the results of measuring the conventional indicators of eggs, the weight of eggs produced by laying hens fed with discarded nutrient medium of Cordyceps militaris was higher than that of laying hens fed with ordinary laying hens. The content of interleukin-1(IL-1) in the experimental group was significantly higher than that in the control group, and the concentration of IL-1 increased by 141.5 pg/mL, which indicated that the application of discarded nutrient medium of Cordyceps militaris effectively improved the immunity of laying hens. The high-throughput analysis of the intestinal contents of the two groups of laying hens showed that the microbial population abundance of the intestinal tract of the experimental group was greater than that of control group, and the application of discarded nutrient medium of Cordyceps militaris increased the diversity of bacteria in the intestinal tract of laying hens. In addition, the sensitivity of some pathogenic bacteria in the intestinal tract of chickens to drugs was also increased, thereby reducing the use of antibiotics. The secondary utilization of discarded nutrient medium of Cordyceps militaris has great development and utilization prospects, which provided a scientific reference and basic theoretical basis for the development of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens.
基金Supported by the Project of Agricultural Science and Technology Achievements Transformation Funds(2014GB2D200212)Agro-scientific Research Programs in Public Interest(201103005)+2 种基金National Germplasm Resource Platform of Green Manure(2012-019)Conservation and Utilization Project of Crop Germplasm(NB2013-2130135-34)Funding Project of International Plant Nutrition Institute(IPNI)(Hunan-16)~~
文摘Five Chinese milk vetch（Astragalus sinicus） varieties were selected to make a comparison in order to screen effective varieties which could suit for the double-rice cropping system of Hunan Province. The results showed that the growth period of Xiangzi 2 is shorter than that of Xiangfei 2, Xiangfei 3 and Yujiangdaye,and is similar to that of Xinyang Chinese milk vetch. The full-bloom stage of Xiangzi 2 is also similar to Xinyang variety and obviously earlier than that of the other three varieties. The fresh grass yield of Xiangzi 2 in full-bloom stage is 23 842.5kg/hm^2, which increases by 39.9% compared with Xinyang variety. Nitrogen content in the fresh grass of Xiangzi 2 is higher than that of the other four varieties. In conclusion, Xiangzi 2 is an extremely early-blossoming and early-maturing Chinese milk vetch variety with moderate fresh grass yield, high nutrient contents, stable characters and wide adaptability. So, Xiangzi 2 is suitable for planting in double-rice cropping region in Hunan Province and other same latitude regions which have similar ecological conditions.
文摘The increasing trend of air temperature along with the climate warming has been accepted gradually by scientists and by the general public. Qinghai_Xizang Plateau, a unique geographic unit due to high_altitude climate, is one of the most susceptible regions to climate warming. Its ecosystem is very fragile and sensitive to climate change. In order to get a better understanding of the impacts of climate warming on the nutrient contents of herbage grown in Qinghai_Xizang Plateau, a simulative study was implemented at Daban Moutain by using temperature differences resulted from sites selected at different altitudes and nutrient contents and in vitro digestibility were determined for assessing the quality of the grown herbage. There were significant downtrends in crude protein (CP), ether extract (EE) and nitrogen free extract (NFE) contents of herbage along with the increase of temperature. It had a positive correlation between temperature and content of acid detergent fibre (ADF), acid detergent lignin (ADL) in herbage. In vitro digestibility of herbage decreased along with the increase of temperature. The results of this study indicated that climate warming significantly influence nutrient contents and in vitro digestibility of herbage grown in Qinghai_Xizang Plateau. It is suggested that the future climate warming especially the gradual rise of the night temperature could cause negative effect on herbage quality grown in Qinghai_Xizang Plateau by decreasing CP, EE, and NFE contents and increasing some indigestible ingredients such as crude fibre (CF), neutral detergent fibre (NDF), ADF, and ADL. This, consequently, decreases the ruminant assimilation ability.
基金Supported by National Natural Science Foundation of China(30900158)Research Foundation for Guangdong Pharmaceutical University(43553006)~~
文摘[Objective] The study had developed a means of rapid propagation Pteris vittata L.by tissue culture. The species was a perennial fern belonging to the genus Pteris. [Metbed] The leaf bud of P. vittata collected in field conditions as explantsand the 1/2 MS ＋ 3% sucrose ＋ 0.7% agar as the basic medium were used to screen the medium formula of the phytohormone ratio for callus induction and subculture of P. vittata. [Result] The best medium formula for each step was list below： 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.1 mg/L KT ＋ 0.5 mg/L 2, 4-D for in- ducing the callus from explants; 1/2MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 1.0 mg/L KT ＋ 0.01 mg/L 2,4-D for inducing the GGB from callus and the seedlings from GGB. In addition, 1/2 MS ＋ 3% sucrose ＋ 0.7% agar ＋ 0.5 g/L PVP ＋ 0.5 mg/L 2,4-D for the subculture could make the continued proliferation of callus. [Cen- clusioa] This study makes an applicable procedure by the direct use of field materi- als, for propagating P. vittata in a simplified and rapid mode.
文摘The line of T240-6 was selected from 32 SC 2 lines of immature embryo culture of T240 (common wheat (Triticum aestivum L.)× Wheat-Haynaldia villosa (L.) Schur. 6D/6V substitution line) through powdery mildew characterization, glutamate oxaloacetate transaminase (GOT) enzyme and gliadin (Gli) analyses and in situ hybridization. All of the individual plants resistant to powdery mildew lacked the locus of GOT at 6VL arm (GOT-V 2) and had gliadin locus at 6VS arm (Gli-V 2) of Haynaldia villosa. All the plants resistant to powdery mildew had one or two telocentric chromosomes that did not pair with wheat chromosomes but paired between themselves. T240-6 was identified as a telocentric line through in situ hybridization.
基金This research was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX2–YW–309)the Major State Basic Research Development Program of China (973 Program No. 2004CB418507)
文摘Seasonal dynamics of above- and belowground biomass and nutrient characteristics （nitrogen, carbon, and phosphorus） of Carex lasiocarpa were investigated in the typical wetland of Sanjiang Plain, China from May 2007 to September 2008. The results show that the changes of aboveground biomass during the growing season are best described by the twice function curve model, whereas the changes of belowground biomass follow the exponential increase curve model. Both the organic carbon contents in the above- and belowground plant parts show significant positive linear correlations with the growing time, and the coefficients R2 are 0.983 and 0.746, respectively. The carbon accu-mulations of the above- and belowground plant parts during the growing season show the same dynamics as those of the biomass. However, the nitrogen contents and accumulation in C. lasiocarpa aboveground and belowground parts show exponential increase during the growing season. The dynamics of C. lasiocarpa phosphorus contents follows the twice function curve model, whereas the accumulation of phosphorus shows the linear increase. The ratios of C/N in different parts of C. lasiocarpa fit the negative linear relations with total nitrogen content in the growing season. Moreover, the ratios of C/P in C. lasiocarpa plant also fit the negative linear relations with total phosphorus content. The results show that nitrogen is the primary limiting nutrient for C. lasiocarpa growth as compared with carbon and phosphorus.
