The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear （Momordica charantia L.） were invest...The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear （Momordica charantia L.） were investigated. Sterilized for 6 rain with 0.1% （w/v） mercuric chloride solution is an essential sterilization method for stems. June and July proved to be better for material taking, followed by May. The callus formation rate of balsam pears reached the highest when medium added with IBA 1.0mg/L and BA 2.0 mg/L, while the optimum medium for adventitious bud induction was MS added with IBA 0.5mg/L and BA 4.0 mg/L. And it will provide theoretical and technical basis for rapid propagation and breeding.展开更多
The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment...The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment was conducted in2011 and 2012 at three to four week intervals from May to October. Air samples were taken from three locations in the Karkonosze Mts. and one from the Izerskie Mts. To examine the air, the Air Ideal 3P sampler and acidified PDA medium were used. The results show that Cladosporium cladosporioides was the most abundant spore type in all the sampling locations(up to 30%), followed by Alternaria alternata(16%–20%), Fusarium(up to 10%) and Sclerotinia sclerotiorium(up to 6%). The lower spore counts were recorded in May samples, compared to the other months. In this case the snow cover, that was still present in the area at the beginning of May,may be the reason for the lower, compared to June,July and August samplings, CFU(Colony Forming Unit) counts in that month.. The influx of air masses from SE, S and SW sectors in the area under study may affect dissemination of the plant-pathogenic fungi from the Czech Republic and from the South of Europe in general.展开更多
文摘The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear （Momordica charantia L.） were investigated. Sterilized for 6 rain with 0.1% （w/v） mercuric chloride solution is an essential sterilization method for stems. June and July proved to be better for material taking, followed by May. The callus formation rate of balsam pears reached the highest when medium added with IBA 1.0mg/L and BA 2.0 mg/L, while the optimum medium for adventitious bud induction was MS added with IBA 0.5mg/L and BA 4.0 mg/L. And it will provide theoretical and technical basis for rapid propagation and breeding.
基金owe a debt of gratitude to the Department of Plant Protection, Wroclaw University of Environmental and Life Sciences for financing the project
文摘The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment was conducted in2011 and 2012 at three to four week intervals from May to October. Air samples were taken from three locations in the Karkonosze Mts. and one from the Izerskie Mts. To examine the air, the Air Ideal 3P sampler and acidified PDA medium were used. The results show that Cladosporium cladosporioides was the most abundant spore type in all the sampling locations(up to 30%), followed by Alternaria alternata(16%–20%), Fusarium(up to 10%) and Sclerotinia sclerotiorium(up to 6%). The lower spore counts were recorded in May samples, compared to the other months. In this case the snow cover, that was still present in the area at the beginning of May,may be the reason for the lower, compared to June,July and August samplings, CFU(Colony Forming Unit) counts in that month.. The influx of air masses from SE, S and SW sectors in the area under study may affect dissemination of the plant-pathogenic fungi from the Czech Republic and from the South of Europe in general.
