IBS内提骨凿行山羊上颌窦内提升效果的实验研究

目的以山羊头为模型,探讨IBS内提骨凿行上颌窦内提升的效果。方法通过解剖山羊上颌窦并分析山羊CBCT,建立直视下于山羊头腭侧壁进行上颌窦内提升的动物实验模型。山羊头24只含48个上颌窦依据不同的上颌窦目标提升高度随机分为提升高度5 mm,8 mm,10 mm三组,每组8只16个上颌窦,使用IBS内提骨凿行上颌窦内提升术,统计并记录每组提升到目标高度时上颌窦是否穿孔、上颌窦穿孔个数以及穿孔时提升高度。结果提升高度5 mm,8 mm,10 mm三组各16个上颌窦穿孔数分别为1,3和9个。上颌窦提升至目标高度5 mm与8 mm组窦膜穿孔率比较差异无统计学意义(P>0. 05),提升高度5 mm与10 mm组差异具有统计学意义(P<0.05),提升高度8 mm组与10 mm组差异具有统计学意义(P<0.05)。相较于提升高度达10 mm,运用IBS内提骨凿进行山羊上颌窦内提升高度5 mm与8 mm穿孔率低,安全性高。结论IBS内提骨凿是一种良好的上颌窦内提升器械,可以安全提升山羊上颌窦膜5-8 mm,使上颌窦膜形成一均匀的伞状膨隆,但提升8 mm以上高度时仍需要慎重。

高职院校辅导员“内提--外促--互学”三位一体培养机制

高职院校辅导员队伍建设既存在诸多不足之处,又面临着许多新情况和新挑战,加强辅导员队伍建设不容忽视。对此,文章提出高职院校辅导员"内提—外促—互学"三位一体培养机制,分别从内提、外促、互学三个方面具体阐述了该培养机制的具体举措和方法,以期为有效提高高职院校辅导员队伍的整体素质提供参考。

高职院校辅导员“内提”培养机制研究

文章针对当前高职院校辅导员培养中存在的问题,提出了构建高职院校学生辅导员"内提"培养机制,分别从四个方面具体阐述了该培养机制的具体举措和方法,实践结果表明,"内提"培养机制能够有效提高高职院校辅导员队伍的整体素质。

高职院校辅导员“内提-外促-互学”三位一体培养机制研究

高职院校学生思想政治工作面临着新的挑战形势下,加强辅导员队伍建设不容忽视。文章提出了高职院校学生辅导员"内提-外促-互学"三位一体培养机制,分别从三个方面具体阐述了该培养机制具体举措和方法,并加以实践。结果表明,"内提-外促-互学"三位一体培养机制能够有效提高高职院校辅导员队伍的整体素质并充分调动其工作积极性。

高职院校辅导员队伍“内提”培养机制探究

加强辅导员队伍建设是高职院校内涵式发展中重要的一环。文章就高职院校学生辅导员"内提"培养机制,分别从五个方面具体阐述了该培养机制的具体举措,旨在培养和建设适合高职教育发展的优秀辅导员队伍。

IBS内提骨凿与Summers骨凿行上颌窦内提升效果比较的实验研究

目的:建立上颌窦内提升的山羊动物实验模型,比较IBS内提骨凿与Summers骨凿进行山羊上颌窦内提升的效果。方法:大量预实验对山羊上颌窦解剖分析,结合山羊CBCT对上颌窦提升位点探讨,建立上颌窦内提升的山羊动物实验模型。对18只本地山羊36个上颌窦左右随机分为实验组与对照组,实验组运用IBS内提骨凿,对照组运用Summers骨凿,18只山羊依据上颌窦提升高度为5,8,10mm随机分为3组,每组6只羊头,统计每组实验组与对照组的穿孔数并记录穿孔时上颌窦提升的高度。结果:建立了以山羊上颌第一磨牙腭侧6-8mm的位点进行上颌窦内提升的动物实验模型。每组实验组6个上颌窦穿孔数分别为0、1、3;对照组6个上颌窦穿孔数分别为1、5、6。实验组与对照组行上颌窦内提升高度为5mm时两者安全性无明显差异[(5.00±0.00) mm vs(4.92±0.20) mm,P>0.05],实验组与对照组行上颌窦内提升高度为8mm时两者差异显著[(7.83±0.41) mm vs (6.58±1.32) mm,P<0.05],实验组与对照组行上颌窦内提升高度为10mm时两者安全性差异显著[(9.17±1.57) mm vs (6.08±0.97) mm,P<0.05]。结论:山羊上颌第一磨牙腭侧6-8mm的位点进行上颌窦内提升效果显著,当提升高度小于5mm,两者提升效果无明显差异,提升高度大于5mm,两骨凿相较而言,IBS内提骨凿较Summers骨凿有更好的提升效果及安全性。

盘县白河沟水利工程设计

白河沟水利工程的主要任务是城市供水,同时兼有灌溉、人畜饮水等综合效益,是红果经济开发区的主要供水水源。坝址河段位于云贵两省交界处,采用库内深井泵站提水,经15 km长输水线路将水送至红果水厂,提水流量1.089 m3/s,最大净扬程88 m。大坝坝型为面板堆石坝,由于坝址两岸地形较陡,不宜布置岸边开敞式溢洪道,所以布置坝顶溢洪道,是贵州省第一座在堆石坝上布置坝顶溢洪道的水利工程。坝顶溢洪道布置于坝体左坝段,单宽流量6 m3/(s·m)。该工程的实施,为在峡谷山区修建面板堆石坝提供了新的思路。

Elicitation on Artemisinin Biosynthesis in Artemisia annua Hairy Roots by the Oligosaccharide Extract from the Endophytic Colletotrichum sp. B501

The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.

Changes of the Transcriptional Levels of Molecules Associated with Endogenous Antigen Processing and Presentation in Porcine Skin-derived Dendritic Cells Infected with PCV2 in vivo

[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.

Hydrodynamics and Solids Mixing Behavior in a Riser with Blunt Internals

The hydrodynamics and solids mixing behavior in a riser with blunt internals are studied. A uniform radial distribution for solids fraction and particle velocity achieves near the internals. The turbulent velocity of particles near the wall increases with the addition of the internals, with the lateral solids mixing enhanced significantly. Probability density distribution of particle velocity is bimodal in the riser with internals, which is similar to that in the conventional riser, indicating that no significant difference in the micro flow structure exists between the riser with internals and the conventional riser. At the same time, the axial solids mixing behavior changes insignificantly with the addition of internals. These results indicate that the micro flow structure in the riser is very stable, which changes insignificantly with the change of the bed structure.

Exploring Hits-Based Content Provisioning Mechanism in Content-Centric Networking

In-network caching and Interest packets aggregation are two important features of Content-Centric Networking(CCN).CCN routers can directly respond to the Interest request by Content Store(CS)and aggregate the same Interest packets by Pending Interest Table(PIT).In this way,most popular content requests will not reach the origin content server.Thus,content providers will be unaware of the actual usages of their contents in network.This new network paradigm presents content providers with unprecedented challenge.It will bring a great impact on existing mature business model of content providers,such as advertising revenue model based on hits amount.To leverage the advantages of CCN and the realistic business needs of content providers,we explore the hits-based content provisioning mechanism in CCN.The proposed approaches can avoid the unprecedented impact on content providers' existing business model and promote content providers to embrace the real deployment of CCN network.

Understanding the YouTube Partners and Their Data： Measurement and Analysis

User generated content,e.g.,from YouTube,the most popular online video sharing site,is one of the major sources of today's big data and it is crucial to understand their inherent characteristics.Recently,YouTube has started working with content providers(known as YouTube partners) to promote the users' watching and sharing activities.The substantial benefit is to further augment its service and monetize more videos,which is crucial to both YouTube and its partners,as well as to other providers of relevant services.In this paper,our main contribution is to analyze the massive amounts of video data from a YouTube partner's view.We make effective use of Insight,a new analytics service of YouTube that offers simple data analysis for partners.To provide the practical guidance from the raw Insight data,we enable more complex investigations for the inherent features that affect the popularity of the videos.Our findings facilitate YouTube partners to re-design current video publishing strategies,having more opportunities to attract more views.

The antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo

To investigate the antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo, six bromophenol derivatives 6-（2,3-dibromo-4,5-dihydroxybenzyl）-2,3-dibromo-4,5-dihydroxy benzyl methyl ether （1）, （＋）-3-（2,3-dibromo-4,5-dihydroxyphenyl）-4-bromo-5,6-dihydroxy-1,3- dihydroisobenzofuran （2）, 3-bromo-4-（2,3-dibromo-4,5-dihydroxybenzyl）-5-methoxymethyl-pyrocatechol （3）, 2,2＇,3,3＇-tetrabromo-4,4＇,5,5＇-tetrahydroxy-diphenylmethane （4）, bis（2,3-dibromo-4,5-dihydroxybenzyl） ether （5）, 2,2＇,3-tribromo-3＇,4,4＇,5-tetrahydroxy-6＇-ethyloxymethyldiphenylmethane （6） were isolated from brown alga Leathesia nana, and their cytotoxicity were tested by MTF assays in human cancer cell lines A549, BGC-823, MCF-7, B16-BL6, HT-1080, A2780, Be17402 and HCT-8. Their inhibitory activity against protein tyrosine kinase （PTK） with over-expression of c-kit was analyzed also by ELISA. The antitumor activity of ethanolic extraction of Leathesia nana （EELN） was evaluated on S180-bearing mice. All compounds showed very potent cytotoxicity against all of the eight cancer cell lines with IC50 below 10 pg/mL. In PTK inhibition study, all bromophenol derivatives showed moderate inhibitory activity and compounds 2, 5 and 6 showed significant bioactivity with the inhibition ratio of 77.5%, 80.1% and 71.4% respectively. Pharmacological studies reveal that EELN could inhibit the growth of Sarcoma 180 tumor and increase the indices of thymus and spleen to improve the immune system remarkably in vivo. Results indicated that the bromophenol derivatives and EELN can be used as potent antitumor agents for PTK over-expression of c-kit and considered in a new therapeutic strategy for treatment of cancer.

Effects of sargentgloryvine stem extracts on HepG-2 cellsin vitro andin vivo

AIM:To observe the effects of sargentgloryvine stem extracts (SSE) on the hepatoma cell line HepG-2 in vitro andin vivo and determine its mechanisms of action.METHODS:Cultured HepG-2 cells treated with SSE were analysed by 3-(4,5-Dimethyl-thiazol-2-yl)-2,5Diphenyltetrazolium bromide and clone formation assay.The cell cycle and apoptosis analysis were conducted by flow cytometric,TdT-Mediated dUTP Nick End Labeling and acridine orange/ethidium bromide staining methods,and protein expression was examined by both reverse transcriptase-polymerase chain reaction and Western blotting.The pathological changes of the tumor cells were observed by haematoxylin and eosin staining.Tumor growth inhibition and side effects were determined in a xenograft mouse model.RESULTS:SSE treatment could not only inhibit HepG-2 cell proliferation in a doseand time-dependent manner but also induce apoptosis and cell cycle arrest at the S phase.The number of colonies formed by SSEtreated tumor cells was fewer than that of the controls (P<0.05).SSE induced caspase-dependent apoptosis accompanied by a significant decrease in Bcl-xl and Mcl-1 and elevation of Bak expression (P<0.05).Tumor necrosis factor α in the xenograft tumor tissue and the liver functions of SSE-treated mice showed no significant changes at week 8 compared with the control group (P>0.05).Systemic administration of SSE could inhibit the HepG-2 xenograft tumor growth with no obvious toxic side effects on normal tissues.CONCLUSION:SSE can induce apoptosis of HepG-2 cells in vitro and in vivo through decreasing expression of Bcl-xl and Mcl-1 and increasing expression of Bax.

The anti-cancer effect of Huaier aqueous extract with rh-Endostatin and DDP

Objective: The aim of our study was to explore the inhibition and apoptosis-inducing effect of the combination of Huaier aqueous extract with recombinant human Endostatin and DDP in human lung adenocarcinoma A549 cells. We also investigated the reversal effect of Huaier aqueous extract in reversing cisplatin resistance in human lung adenocarcinoma A549/DDP cells. Methods: We treated A549 cells with Huaier aqueous extract and the combination of Huaier aqueous extract and DDP or rh-Endostatin for 24 h, 36 h and 48 h. And then we calculated the inhibition rate through MTT approach and detected the apoptosis rate by flow cytometry. We also treated A549 and A549/DDP cells with DDP, Huaier aqueous extract, DDP and Huaier aqueous extract for 72 h, respectively. Results: Huaier aqueous extract can inhibit the growth of A549 cells and the inhibition rate improved with the increase of the concentration. The inhibition rate of the combination of rh-Endostatin and 4 mg/mL of Huaier aqueous extract in three time points and the combination of rh-Endostatin and 2 mg/mL of Huaier aqueous extract in the time point of 48 h on the growth of A549 cells all improved(P < 0.005). The inhibition rate of the combination of DDP and Huaier aqueous extract with the concentration of 2 mg/mL or 4 mg/mL on the growth of A549 cells all improved(P < 0.005). The combination of Huaier aqueous extract and DDP and the combination of Huaier aqueous extract with rh-Endostatin and DDP can improve the inhibition on the growth of A549 cells(P < 0.005). Conclusion: Huaier aqueous extract has the inhibition and apoptosis-inducing effects on the A549 cells. And the combination of Huaier aqueous extract and rh-Endostatin or DDP has the synergistic effects on the inhibition of A549 cells. The combination of Huaier aqueous extract with rh-Endostatin and DDP has the synergistic effects on the inhibition of A549 cells. Huaier aqueous extract can reverse the cisplatin resistance in human lung adenocarcinoma A549/DDP cells.

Hydrodynamics and Lateral Gas Dispersion in a High-Density Circulating Fluidized Bed Reactor with Bluff Internals

Effect of bluff internals on the hydrodynamics and lateral gas mixing was studied in a 0.186m ID high-density riser. With the bluff internals, the extremely non-uniform radial profiles of solid fraction and particle velocity become flat and the dense downflow layer near the wall disappears, indicating the significant enhancement of solid turbulence introduced by the internals. The fluctuation velocity and solid fraction transient signal analysis indicates a significant increase in fluctuation intensity near the wall region. The length influenced by the internals on the flow structure is about 1 meter. The lateral gas dispersion coefficient increases significantly as the bluff internals exist in the riser.

Morphogenesis of Oil Palm Fruit （Elaeis guineensis Jacq.） in Mesocarp and Endocarp Development

This work aims to study the development of the pericarp of the fruit of Elaeis guineensis Jacq. var. dura. The thickness, the water and the oil contents of its tissues are evaluated every two weeks, from pollination to the maturity of the fruit. The development of the oil palm fruit takes 5.5 months. The endocarp reaches its maximum thickness at the 70th DPP （day post-pollination）, with a water content of 72%. It then starts its dehydration, while sclerifying. It therefore isolates the seed at start and later protects it. The mesocarp is visible at anthesis and its water content is close to 92%. From the 100th DPP, it begins a continuous dehydration associated, from the 130th DPP, with an active lipids biosynthesis. Ultimately, the pericarp of the oil palm fruit fulfills both functions, namely to protect the seed by early sclerification of the endocarp and ensure the dissemination of the species by the high oil content of the mesocarp. A comparative anatomy of the pericarp tissues of the three genotypes.of E. guineensis Jacq., during the first three weeks of fruit development, will enhance the understanding of the primary effect of sh gene.

Antifi brotic effects of green tea on in vitro and in vivo models of liver fibrosis

AIM: To examine the protective effect of green tea extract (GT) on hepatic fi brosis in vitro and in vivo in dimethylnitrosamine (DMN)-induced rats.METHODS: HSC-T6, a rat hepatic stellate cell line, was used as an in vitro assay system. Cell proliferation, collagen content, and type 1 collagen expression were examined in activated HSC-T6 cells. Collagen was determined by estimating the hydroxyproline content. In rats with DMN-induced hepatic fi brosis, serum aspartate aminotransferase and alanine aminotransferase concentrations, liver hydroxyproline and lipid peroxides were determined. Pathologic changes were examined by hematoxylin & eosin staining.RESULTS: GT administration prevented the development of hepatic fibrosis in the rat model of DMN-induced liver fi brosis. These results were confi rmed both by liver histology and by quantitative measurement of hepatic hydroxyproline content, a marker of liver collagen deposition. Accordingly, inhibition of proliferation, reduced collagen deposition, and type 1 collagen expression were observed in activated HSC-T6 cells following GT treatment. These results imply that GT reduced the proliferation of activated HSC and down regulated the collagen content and expression of collagen type 1, thereby ameliorating hepatic fibrosis.CONCLUSION: This study demonstrates that greentea administration can effectively improve liver fibrosis caused by DMN, and may be used as a therapeutic option and preventive measure against hepatic f ibrosis.

Structures and Antiviral Activities of Butyrolactone Derivatives Isolated from Aspergillus terreus MXH-23

A new butyrolactone derivative,namely butyrolactone Ⅷ （1）,and six known butyrolactones （2-7） were separated from the ethyl acetate （EtOAc） extract of the fermentation broth of a fungus,Aspergillus terreus MXH-23.The chemical structures of these metabolites were identified by analyzing their nuclear magnetic resonance （NMR） and mass spectrometry （MS）.Known butyrolactone derivatives contain an α,β-unsaturated γ-lactone ring with α-hydroxyl and y-benzyl,and butyrolactone Ⅷ （1） was the first butyrolactones contains α-benzyl and γ-hydroxyl on α,β-unsaturated lactone ring.All of the butyrolactone derivatives were tested for their anti-influenza （H 1N 1） effects.Derivatives 4 and 7 showed moderate antiviral activities while the newly-identified,derivative 1,did not.

In Vivo Anti-Inflammatory Assessment of Quillaja saponaria Mol. Extracts

Aqueous extracts from the native Chilean Quillaja saponaria tree are used orally and topically to treat lung inflammatory problems and toothache. This study aimed to corroborate their presumed anti-inflammatory activity, identify their active ingredients and validate their use. The topical anti-inflammatory activity of a commercial partially purified Quillaja saponin aqueous extract （Ultra Dry 100Q, UD）, the crude acid hydrolysate （H-100Q） of this material, its two most abundant sapogenins QA （quillaic acid） and PA （phytolaccagenic acid）, and two formulations containing QA were subjected to topical assays for the inhibition ofmurine ear inflammation elicited by AA （arachidonic acid） or TPA （phorbol ester）. The dose-dependent anti-inflammatory activity of QA was confirmed in both AA （maximal effect 92.1%） and TPA （maximal effect 62.2%） assays, and PA showed significant anti-inflammatory activity against AA （46.5%）. Two dermo pharmaceutical formulations containing 8% w/v QA as the active ingredient--a cream and a gel--also exhibited significant anti-inflammatory effects in the TPA （50.8%） and AA （39.5%） assays.