Objective:To study the effect of electrical stimulation of efferent vagus nerve on the acute liver injury induced by endotoxemia in rabbits. Methods:Sixteen rabbits were randomly divided into stimulation group(Group ...Objective:To study the effect of electrical stimulation of efferent vagus nerve on the acute liver injury induced by endotoxemia in rabbits. Methods:Sixteen rabbits were randomly divided into stimulation group(Group A,n=8) and control group (Group B,n=8).They were subjected to bilateral cervical vagotomy and intravenously challenged by lipopolysaccharide (LPS) (E.coli 0111:B4,DIFCO,USA) at a dose of 100 μg/kg injected within 30 min.The distal end of the left vagus nerve trunk was placed across bipolar electrodes connected to a stimulation module and controlled by an acquisition system.Stimuli with constant voltage (10V,5Hz,5ms) were applied twice to the nerve for 10 min before and after the administration of LPS in Group A.At the time 30,60,120,180,240,300 min before and after infusion of LPS respectively in each animal,blood samples were taken for late measurement of the serum Alanine aminotransferase (ALT),Aspartate aminotransferase (AST),tumor necrosis factor-α(TNF-α) and interleukin-10 (IL-10).Immediately after the experiment was finished,autopsy was performed and liver samples were taken to pathologic study. Results:Compared with Group B,the electrical stimulation of efferent vagus nerve could significantly decrease the contents of ALT,AST and TNF-α,but increase the contents of IL-10,in serum of Group A.It could also alleviate inflammation of liver tissue after LPS attack. Conclusion:The results suggest that excitation of the efferent vagus nerve can inhibit the inflammation cascade in liver after LPS challenge.Thus,it might have a protective effect on acute liver damage caused by endotoxemia.展开更多
Objective ： To evaluate the influence of preoperatively selected gut decontamination （SGD） on intestinally derived endotoxemia （ETM） in patients with rheumatic heart disease undergoing valve replacement operation...Objective ： To evaluate the influence of preoperatively selected gut decontamination （SGD） on intestinally derived endotoxemia （ETM） in patients with rheumatic heart disease undergoing valve replacement operation with cardiopulinonary bypass （ CPB ） . Methods ： Thirty patients were randomly divided into control group and SGD group. The patients in control group underwent preoperative bowel preparation, i. e, diet preparation and enema. The patients in SGD group were administrated 100 mg Tobramycin, 40 ing garlicin and 20 % Lactulose for 10 ml three times per day for 3 days besides routinely preoperative bowel preparation. Bacteria cultivation and identification and Gram staining of feces in both groups were used to evaluate species of intestinal flora and their ratios. The levds of endotoxin, D-lactate, TNF-α and complement 3 were determined at four time points of anesthetic induction, CPB end, 2 h after CPB, 24 h after CPB. And the related clinical biochemical and clinical markers were recorded. Resnits - Aerobic gram-negative bacilli （AGNB） ratio in post-SGD group decreased significantly as compared with that in control group and pre-SGD group （ P 〈 0.05 ）. The level of D-lactate reduced significantly at time points of anesthetic induction and 2 h after CPB （P 〈 0. 05 ）. Endotoxin levels of patients in both groups elevated significantly after CPB （P 〈0.05 ）, and endotoxin levels of the patients in SGD group decreased significantly at points ofCPBend （P〈0.01） and 24 h after CPB （P〈0.05） compared with those in control group. The levels of TNF-α and complement 3 were similar in both groups as well as clinical and biochemical markers. Conclusions： CPB induces endotoxemia, while the regime of SGD is an effective way to prevent endotoxemia but may not affect activation of inflammatory media and clinical outcomes.展开更多
文摘Objective:To study the effect of electrical stimulation of efferent vagus nerve on the acute liver injury induced by endotoxemia in rabbits. Methods:Sixteen rabbits were randomly divided into stimulation group(Group A,n=8) and control group (Group B,n=8).They were subjected to bilateral cervical vagotomy and intravenously challenged by lipopolysaccharide (LPS) (E.coli 0111:B4,DIFCO,USA) at a dose of 100 μg/kg injected within 30 min.The distal end of the left vagus nerve trunk was placed across bipolar electrodes connected to a stimulation module and controlled by an acquisition system.Stimuli with constant voltage (10V,5Hz,5ms) were applied twice to the nerve for 10 min before and after the administration of LPS in Group A.At the time 30,60,120,180,240,300 min before and after infusion of LPS respectively in each animal,blood samples were taken for late measurement of the serum Alanine aminotransferase (ALT),Aspartate aminotransferase (AST),tumor necrosis factor-α(TNF-α) and interleukin-10 (IL-10).Immediately after the experiment was finished,autopsy was performed and liver samples were taken to pathologic study. Results:Compared with Group B,the electrical stimulation of efferent vagus nerve could significantly decrease the contents of ALT,AST and TNF-α,but increase the contents of IL-10,in serum of Group A.It could also alleviate inflammation of liver tissue after LPS attack. Conclusion:The results suggest that excitation of the efferent vagus nerve can inhibit the inflammation cascade in liver after LPS challenge.Thus,it might have a protective effect on acute liver damage caused by endotoxemia.
文摘Objective ： To evaluate the influence of preoperatively selected gut decontamination （SGD） on intestinally derived endotoxemia （ETM） in patients with rheumatic heart disease undergoing valve replacement operation with cardiopulinonary bypass （ CPB ） . Methods ： Thirty patients were randomly divided into control group and SGD group. The patients in control group underwent preoperative bowel preparation, i. e, diet preparation and enema. The patients in SGD group were administrated 100 mg Tobramycin, 40 ing garlicin and 20 % Lactulose for 10 ml three times per day for 3 days besides routinely preoperative bowel preparation. Bacteria cultivation and identification and Gram staining of feces in both groups were used to evaluate species of intestinal flora and their ratios. The levds of endotoxin, D-lactate, TNF-α and complement 3 were determined at four time points of anesthetic induction, CPB end, 2 h after CPB, 24 h after CPB. And the related clinical biochemical and clinical markers were recorded. Resnits - Aerobic gram-negative bacilli （AGNB） ratio in post-SGD group decreased significantly as compared with that in control group and pre-SGD group （ P 〈 0.05 ）. The level of D-lactate reduced significantly at time points of anesthetic induction and 2 h after CPB （P 〈 0. 05 ）. Endotoxin levels of patients in both groups elevated significantly after CPB （P 〈0.05 ）, and endotoxin levels of the patients in SGD group decreased significantly at points ofCPBend （P〈0.01） and 24 h after CPB （P〈0.05） compared with those in control group. The levels of TNF-α and complement 3 were similar in both groups as well as clinical and biochemical markers. Conclusions： CPB induces endotoxemia, while the regime of SGD is an effective way to prevent endotoxemia but may not affect activation of inflammatory media and clinical outcomes.
