超压力负荷诱导肥厚心肌的肌浆网钙摄取和释放的改变

目的探讨超压力负荷下左心室心肌肌浆网钙ATP酶、雷诺定受体2(ryanodine receptor2,RYR2)和三磷酸肌醇受体1(inositol1,4,5-trisphosphate receptor1,IP3R1)变化以及血管紧张素Ⅱ受体阻断药的影响。方法用腹主动脉缩窄法建立大鼠超压力负荷模型。检测心肌肌浆网钙ATP酶活性、Ca2+最大摄取速率、Ca2+最大摄取量、3H-雷诺定与RYR2最大结合量和RYR2受体密度。用免疫印迹法检测心肌肌浆网钙ATP酶2a(SERCA2a)蛋白表达;用反转录-聚合酶链反应检测心肌RYR2和IP3R1的mRNA表达。结果高压力负荷下左心室心肌呈典型的肥厚心肌的形态改变。手术组左心室心肌内浆网钙ATP酶活性、Ca2+最大摄取速度、Ca2+摄取量、RYR2最大结合量、RYR2的mRNA表达[吸光度/磷酸甘油醛脱氢酶]、IP3R1mRNA表达[吸光度/磷酸甘油醛脱氢酶]均低于假手术组(差异有统计学意义,P<0.01,n=12),缬沙坦组高于手术组及PD123319组(P<0.05),手术组与PD123319组间差异无统计学意义(P>0.05,n=12)。结论超压力负荷诱导的肥厚心肌组织钙调节能力明显下降,但缬沙坦可改善肥厚心肌组织的钙调节能力。

镍电极MLCC内电极浆料与介质烧成收缩匹配性的研究

文章通过实验,阐述了电容器用镍内电极浆料中,有机固含量的大小、无机添加剂的类型、镍/无机添加剂比例对镍内电极浆料烧成收缩曲线,以及镍浆所形成电极层与介质层在烧成过程中收缩率匹配性能的影响。

内蒙古好老鹿场地区晚古生代超基性-基性岩的发现及意义

在内蒙古东部好老鹿场地区1：5万区调工作中，发现了一套新的岩石组合，分别由铁镁质堆积岩、基性岩墙群、蚀变基性火山岩等组成。该套岩石组合经较强的构造破坏，呈大小不等的岩块产于本巴图组砂板岩之中。总体延伸方向呈近东西向展布，明显受区域性构造控制。岩石化学、岩石地球化学等方面的资料表明，辉石橄榄岩、辉长岩具有低Al、贫Ca和富Na的特点，MgO／（MgO＋FeO）比值为0．25，MgO含量偏低。稀土元素总量偏低，为76．03×10^-6～114．81×10^-6，轻重稀土富集不明显，分馏作用较弱。∑L／∑H为0．73～0．8，配分曲线呈平坦的“W”型，δEu=1．03～1．23，略显正异常。基性火山岩具明显的富Fe、Mg、Na，贫Al、Ti、K的特征，微量元素显示形成于洋中脊环境。同时在辉长岩中获得单颗粒锆石U-Pb年龄为362．4Ma，时代为早泥盆世晚期。其形成环境为洋中脊，属于泥盆纪古亚洲洋消减部分的残留体。

Clinical Diagnosis Technique of Goat Pox Disease

[Objective] The aim was to provide theoretical basis for effective prevention of goat pox disease.[Method] 5 cases of infected goats were diagnosed for goat pox with microbiology examination.The poxes on their skin,rumen,reticulum,omasum,abomasum and submandibular lymph nodes,bronchial lymph nodes,lung and spleen were macroscopically and microscopically observed with pathanatomical and histopathological technique.[Result] Poxes on skin mainly showed ashen hemisphere state and gave prominence to the surface of skin; some cases had hemorrhage in the poxes and showed dark purplish red.Poxes on gastric mucosa showed ashen.Cytoplasmic inclusion body could be all observed in epithelial cells of the poxes and macrphages of lymph node,lung and spleen.[Conclusion] Poxes on skin,lung and the surface of gastric mucosa as well as cytoplasmic inclusion body in the epithelial cells of pox and the macrphages of lymphoid organs were the especial pathochanges of goat pox,which could be taken as the proof of goat pox＇s clinic diagnisis.

MLCC制作过程中分层开裂原因分析

MLCC是经过多层材料堆叠共烧后制成的,在其制作过程中,产生分层是比较严重的质量缺陷之一,严重影响MLCC在使用过程中的可靠性。对MLCC产生分层的原因进行分析归类,总结出MLCC产生分层的原因为:制作MLCC内浆与瓷粉TMA(热机械分析)曲线匹配性不佳;MLCC在烧结前存在排胶不良;烧结回火温度或氧含量高。

Possible Mechanism of Effects of Etimicin and Gentamicin on Intracellular Calcium Homeostasis

Aim Intracellular calcium ([Ca^(2+) ]_i) is mainly regulated by mitochondriaand endo-plasmic reticula. This study was carried out to ascertain whether the elementary mechanismof the effects of etimicin (EM) and gentamicin (GM) on [Ca^(2+) ]_i is related to their effects onmitochondrion Ca^(2+) -uptake and endoplasmic reticulum Ca^(2+) -uptake. Methods The effects of GMand EM on [Ca^(2+) ]_i in LLC-PK1 were determined with a fluorescent probe of Fura-2/AM. The effectsof EM and GM on mitochondrion Ca^(2+) -uptake and endoplasmic reticulum Ca^(2+) -uptake weredetermined by isotope indicator (^(45)Ca^(2+) ) . Results EM and GM at the concentration of 1mmol·L^(-1) had no significant effect on [Ca^(2+) ]_i(P. > 0.05) and at 10 mmol·L^(-1)significantly caused [Ca^(2+) ]_i to increase (P < 0.01). EM and GM at 1 mmol·L^(-1) causedmitochondrion Ca^(2+)-uptake to ascend dramatically (P < 0.05) and at 10 mmol·L^(-1) causedmitochondrion Ca^(2+) -uptake to descend significantly. EM and GM at more than 0.34 mrnol·L^(-1)significantly inhibited endoplasmic reticulum Ca^(2+) -uptake (P < 0.05 or 0.01). Conclusion Novariation of [Ca^(2+) ]_i caused by EM and GM at lower concentrations might relate to theequilibrium of their promotion of mitochondrion Ca^(2+) -uptake with their inhibition of endoplasmicreticulum Ca^(2+) -uptake. The elevation of [Ca^(2+) ]_i caused by EM and GM at higherconcentrations might correlate with their inhibition of mitochondrion Ca^(2+) -uptake andendoplasmic reticulum Ca^(2+) -uptake.

Investigation on Risk Factors of Cerebrocardiac Vascular Thrombotic Diseases in Patients with Obstructive Sleep Apnea Hypopnea Syndrome

Objective: To observe the risk factors of thrombosis in patients with obstructive sleep apnea hypopnea syndrome (OSAHS).Methods: From February 2001 to April 2003, 24 patients with moderate and severe OSAHS (OSAHS group) and 19 healthy adults (control group) were recruited. Their blood samples were drawn at 6∶00 and 16∶00 respectively for testing hemocrit, platelet aggregation (PAG), whole blood viscosity (WBV), prothrombin time (PT), activated partial thromboplastin time (APTT), plasma fibrinogen (Fng) and endothelin (ET).Results: There was a significantly higher hemocrit, WBV, Fng and ET as well as a significant shortening of PT and APTT at 6∶00 than that at 16∶00 in OSAHS group. However, there was no significant difference in all testing items between 6∶00 and 16∶00 in control group. The hemocrit, WBV, PAG, plasma Fng and ET were significantly higher, and PT and APTT were obviously shorter at 6∶00 in OSAHS group than those at 6∶00 in control group. A higher hemocrit, PAG, plasma Fng and ET, a longer PT and APTT were observed at 16∶00 in OSAHS group, compared with those at 16∶00 in control group.Conclusion: In OSAHS patients there were striking risk factors of thrombosis, which is more remarkable in the early morning than in the afternoon.

Effects of Magnetic Needle Acupuncture on Blood Pressure and Plasma ET-1 Level in the Patient of Hypertension

Endothelin-1 (ET-1) is ever known as a bio-peptidewith the strongest vasoconstrictive action and withwide biological effects.

Impact of Helicobacter pylori infection on ghrelin and various neuroendocrine hormones in plasma

AIM: Ghrelin, an endogenous ligand for growth hormone secretagogue receptor, influences appetite, energy balance, gastric motility and acid secretion. The stomach is the main source of circulating ghrelin. There are inconsistent reports on the influence of Helicobacter pylori (H pylori) infection on circulating ghrelin levels. We sought to elucidate the relationship between ghrelin and various peptides in plasma, with special reference to H pylori.METHODS: Plasma ghrelin levels were measured by radioimmunoassay in 89 subjects who were referred for upper gastrointestinal endoscopy, consisting of 42 H pylori infected and 47 uninfected ones. Plasma gastrin,somatostatin, leptin, insulin-like growth hormone 1 (IGF-1)and chromogranin A concentrations were also measured.Twelve patients were treated with anti- H pylori regimen.RESULTS: Ghrelin circulating levels were greatly decreased in H pylori-positive than negative individuals (194.2±90.2fmol/mL and 250.4±84.1 respectively, P＜0.05), but did not significantly alter following the cure of infection (176.5±79.5 vs 191.3±120.4). There was a significant negative correlation between circulating ghrelin and leptin levels, as well as body mass index, for the whole and uninfected population, but not in H pylori-infected patients. Plasma ghrelin concentrations correlated positively with IGF-1 in H pylori-negative group and negatively with chromogranin A in the infected group.There were no significant correlations among circulating levels of ghrelin, gastrin and somatostatin irrespective of H pylori status.CONCLUSION: H pylori infection influences plasma ghrelin dynamics and its interaction with diverse bioactive peptides involved in energy balance, growth and neuroendocrine function.

Effects of psychological stress on small intestinal motility and expression of cholecystokinin and vasoactive intestinal polypeptide in plasma and small intestine in mice

AIM: To investigate the effects of psychological stress on small intestinal motility and expression of cholecystokinin (CCK) and vasoactive intestinal polypeptide (VIP) in plasma and small intestine, and to explore the relationship between small intestinal motor disorders and gastrointestinal hormones under psychological stress. METHODS: Thirty-six mice were randomly divided into psychological stress group and control group. A mouse model with psychological stress was established by housing the mice with a hungry cat in separate layers of a two-layer cage. A semi-solid colored marker (carbon-ink) was used for monitoring small intestinal transit. CCK and VIP levels in plasma and small intestine in mice were measured by radioimmunoassay (RIA). RESULTS: Small intestinal transit was inhibited (52.18±19.15% vs70.19±17.79%, P<0.01) in mice after psychological stress, compared to the controls. Small intestinal CCK levels in psychological stress mice were significantly lower than those in the control group (0.75±0.53 μg/g vs1.98±1.17 μg/g, P<0.01), whereas plasma CCK concentrations were not different between the groups. VIP levels in small intestine were significantly higher in psychological stress mice than those in the control group (8.45±1.09 μg/g vs7.03±2.36 μg/g, P<0.01), while there was no significant difference in plasma VIP levels between the two groups. CONCLUSION: Psychological stress inhibits the small intestinal transit, probably by down-regulating CCK and up-regulating VIP expression in small intestine.

CHANGES OF PLASMA ENDOTHELIN CONCENTRATIONS BEFORE AND AFTER PERCUTANEOUS BALLOON MITRAL VALVULOPLASTY IN PATIENTS WITH MITRAL STENOSIS

Plasma concentrations of endothelin in bloor from the femoral vein and the antecubital vein were measured in 35 patients with mitral stenosis and heart failure before and after percutaneous balloon mitralvalvuloplasty(PBMV). The basal plasma concentrations of endothelin in blood from the antecubirtal vein in the patients were significantly higher than those in 32 control subjects (15. 40± 3. 32 vs. 9. 59± 2. 66 pg/ml, P<0. 001). Plasma endothelin concentrations in patients in New York Heart Association functional classes Ⅱ and Ⅲ were significantly higher than those in control subjects, respectively. The concentrations of endothelin in patients with atrial fibrillation were also significantly higher than those in patients with normal sinus rhythm. Ten to fifteen minutes after PBMV, plasma endothelin concentrations in blood from the femoral vein significantly decreased from 16. 14 ± 3. 34 to 13. 74 ± 3. 78 pg/ml (P<0. 01 ). Seventy-two hours after the procedure, the concentrations of endothelin in blood from the antecubital vein had fallen to 12. 31 ± 2. 55 pg/ml (P<0. 001 vs. before PBMV and control subjects). Plasma endothelin concentrations still tended to be higher in patients with atrial fibrillation than those in normal sinus rhythm, but the difference did not reach statistical significance. There were weak but significantly correlations of plasma endothe lin concentrations with the mean left atrial pressure (r= 0. 424 , P < 0.001 ), mean right atrial pressure (r=0. 323, P<0. 01), mean transmitral pressure gradient (r= 0. 397, P<0. 001), heart rate (r= 0. 350,P<0. 005)and mitral valve area (r=-0. 454, P<0. 001) in the patients before and after PBMV.

CHANGES OF PLASMA ENDOTHELIN AND ATRIAL NATRIURETIC PEPTIDE DURING THE ONSET AND AFTER TERMINATION OF PAROXYSMAL SUPRAVENTRICULAR TACHYCARDIA

Radioimmunoassays were used to measure the concentration changes of plasma endothelin(ET) and atrial natriuretic peptide(ANP) during the onset and after termination of paroxysmal supraventricular tachycardia(SVT). 30 cases were reviewed and compansons with 42 normal subjects were made. There are very significant differences(P<0.0001) in the concentration changes of both plasma ET and ANP during the onset and 30 minutes after the termination of SVT. During the onset period of SVT. the plasma ET and ANP were markedly elevated and 30 minutes after its termination they were lowered significantly, but their concentrations were still 2-fold higher than ihose of the control group. As the biological effects of ANP and ET are antagonistic to each other. their parallel elevation and lowering of plasma concentrations during and.after the termination of SVT reveal that these 2 hormones parucipate in the pathophysiological process of SVT. This phenomenon is possibly one of the homeostatic regulatory functions in the organism.

In vivo effects of 17-β-estradiol on plasma immunoglobulin levels and leukocyte density in zebrafish Danio rerio

Estradiol, or 17-β-estradiol （E2）, the most potent naturally occurring estrogen, is involved in the hormone-immune system interaction in both mammals and fish. However, in vivo studies are largely limited, and little is known about whether E2 exerts similar effects on both female and male zebrafish （Danio rerio）. Here, we show exposure of both sexes ofD. rerio to 20 nmol/L E2 resulted in a significant increase in Vgl expression, but caused little damage to the hepatocytes, suggesting that this is the optimum E2 concentration. Also, exposure to 20 nmol/L E2 for 20 days caused a marked increase in plasma IgM levels, but had little influence on the peripheral leukocyte density, providing the first evidence of a hormone-immune system interaction in this species.

Normalization of plasma growth hormone alleviated malignant ventricular tachycardia in acromegaly

Acromegaly is an insidious endocrine disease character- ized by chronic elevation of growth hormone （GH） and insulin-like growth factor-1 （IGF-1）.Ell Persistent excess se- cretion of GH and IGF-1 damages both cardiac structure and function, leading to acromegalic cardiomyopathy, which is one of the most common causes of increased mortality in acromegaly and can result in an average of 10-year reduction in life expectancy.I2＇31 In patients with acromegaly, approximately 3% have been reported having a unique cardiomyopathy characterized by biventricular hypertrophy, myocardial necrosis, lymphocytic infiltration, interstitialfibrosis.

THE INCREASE IN PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 EXPRESSION BY STIMULATION OF ACTIVATORS FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTHELIAL CELLS

Objective.To investigate the effect of peroxis ome proliferator-activated recept ors(PPARs )activators on plasminogen activator inhibitor ty pe-1(PAI-1)expression in human umbilical vein e ndothelial cells and the possi-ble mechanism.Methods.Human umbilical vein endothelial ce lls(HUVECs )were obtained from normal fetus,and cul-tured conventionally.Then the HUVECs were exposed to test agents(linolenic acid,linoleic acid,oleic acid,stearic acid and prostaglandin J 2 respectively)in varying concentrations with fresh media.RT -PCR and ELISA were applied to determine the expression of PPARs and PAI-1in HUVECs.Results.PPARα,PPARδand PPARγmRNA were detected by using RT-PCR in HUVECs.Treatment of HUVECs with PPARαand PPARγactivators---linolenic acid,linoleic acid,oleic acid and prostaglandin J 2 respectively,but not with stearic a cid could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner.However,the mRNA expressions of 3subclasses of PPAR with their activators in HUVECs were not changed compared w ith controls.Conclusion.HUVECs express PPARs.PPARs activators may increase PAI-1expression in ECs,but the underlying mechanism remains uncle ar.Although PPARs expression was not enhanced after stimulated by their activators in ECs,the role of functionally active PPARs in regulating PA I-1expression in ECs needs to be further investigated by using transient gen e transfection assay.

Cerebrospinal fluid and plasma propofol concentration during total intravenous anaesthesia of patients undergoing elective intracranial tumor removal

Objective： The aim of this paper is to compare the propofol concentration in plasma and cerebrospinal fluid （CSF） in patients scheduled for intracranial tumor removal and anaesthetized using propofol as part of a total intravenous anaesthesia technique. Methods： Twenty-seven patients （ASA Ⅰ-Ⅱ） scheduled for elective intracranial tumor removal were studied. Anesthesia was induced with 2 mg/kg propofol for 5 min and infused at 10 mg/（kg·h） for 5 min and then stopped. CSF and arterial blood were collected simultaneously before infusion of propofol and at different time points after infusion ofpropofol according to bispectral index （BIS） values. Concentrations of propofol in plasma and CSF were measured by HPLC with fluorescence detection. The correlation coefficient and regression equation between plasma and CSF concentration of propofol were worked out by linear simple regression. Results： The propofol CSF concentration that we measured was 1.46% of the plasma concentration. The coefficient of relation between plasma and CSF concentration was 76.7%. Conclusions： The propofol CSF concentration was positively correlated with and much lower than the plasma concentration. Discrepancies may result from high plasma protein binding of propofol, intracranial pathology and sampling volume.

Geology and geochemistry of Huanggangliang stratabound skarn deposit in Inner Mongolia,China

Over the recent three decades, exploration of a large-size Sn-Fe polymetallic ore deposit at the Huanggangliang, the Da Hinggan Mountains, Inner Mongolia, China, has been largely focused on the premise that the mineralization represents epigenetic magmatic hydrothermal ore deposit in genetic connection with the Mesozoic magmatism. The Huanggangliang Sn-Fe polymetallic ore deposits occurred in Permian strata, with silt- stone/marble of the Zhesi Formation and spilite/andesite/tuff of the Dashizhai Formation. The characteristics of geological and geochemical data demonstrated that sedimentary hydrothermal mineralization occurred during the basin evolution at the Permian, rather than representing epigenetie magmatic hydrothermal genesis with the following evidences. The ore-bearing skarus are stratiform with underlying metasedimentary rocks and overlying volcanic sedimentary rocks. Sedimentary hydrothermal textures and structures are observed in the stratabound ore-bearing skarn such as bedded-laminated skarn and magnetite ores with small-size folding or soft deforma- tion, synsedimentary breccia of skarn and magnetite ores and concentric shell structure of magnetite ores. So the stratabound ore-bearing skarns associated with magnetite ore and micro-disseminated tin, are peculiar examples of exhalite. The REE contents of the stratabound ore-bearing skarns display ∑LREE-rich （36.91×10^-6） but EHREE-depletion （6. 42 ×10^-6） , with positive Eu anomaly （Eu/Eu ＊ 1.28） and negative Ce anomaly （Ce/ Ce ＊ 0.88 ） , which is totally different from REE pattern of the Huanggang magmatic rocks（ with ∑REE 277.73 ×10^-6, ELREE 220.24 ×10^-6, ∑HREE 57.49 ×10^-6, Eu/Eu＊ 0.06, Ce/Ce＊ 1.52） , which is comparable with modern sea-floor hydrothermal fluid,sedimentary hydrothermal ore deposit and associated hydrothermal sedimentary rocks. Calcite samples with δ13CPDB from -5. 400 ‰ to -4. 397 ‰ and δ18SOSMOW from 9. 095 ‰ to 9. 364 ‰ in the stratabound ore-bearing skarns show sedimentary hydrothermal genesis of the Huanggangliang deposit. This proposition is useful not only for interpretation of the genesis of the Huanggangliang large Sn-Fe polymetallic ore deposit but also significant for mineral exploration in the area especially for finding large deposits caused by sedimentary exhalative mineralization processes.

Malignant neoplasms of the uterus following radiation therapy for cervical carcinoma: a clinical study of 47 cases

Objective： To study the characteristics and clinical features of uterine neoplasms developed after radiation therapy for cervical carcinoma. Methods： Clinical data of 47 cases of uterine neoplasms occurred following radiation therapy for cervical carcinoma were retrospectively reviewed. Results： The median age at uterine neoplasms diagnosis was 62 years （range： 38-77 years）, and the median latency period from initial therapy to development of uterine neoplasms was 14 years （range： 5-35 years）. Thirty of 47 cases were endometrial carcinoma, of which 3 were uterine papillary serous carcinoma （UPSC）. Seventeen of 47 patients were uterine sarcoma, all of those were carcinosarcoma. The distribution by stage, grade, and histology of 30 cases of endometrial carcinoma was as follows： stage Ⅰb, 1 case; stage Ⅰc, 2 cases; stage Ⅱ, 6; stage Ⅲa, 4; stage Ⅲb, 2; stage Ⅲc, 11; stage Ⅳ, 4 cases; grade 1, two cases; grade 2, nine; grade 3 （include 3 UPSC patients）, seventeen; unknown grade, two; endometriod, 27; UPSC, 3 cases; 7 of 30 cases of endometrial carcinoma had recurrences （23.3%）, at median time to recurrence was 24 months, and their median survival time was 26 months. The overall 3- and 5-year survival rates were 60% and 38%, respectively. Of the 17 cases of uterine sarcoma, the median survival was 10 months, 6 patients occurred recurrence （35.9%）, at a median time to recurrence was 9 months, and their median survival was 6 months. The overall 3- and 5-year survival rates were 12% and 0, respectively. Conclusion： The main uterine neoplasms development after radiation therapy for cervical carcinoma is endometrial carcinomas, of which there is a preponderance of high-risk histological subtypes and a poor prognosis. Most of the uterine sarcomas occurred following radiation therapy for cervical carcinoma are carcinosarcomas and the prognosis is very poor.

The Development of Undaria Pinnatifida Bean Curd

Using Undaria pinnatifida juice and soya bean as main material, glucono delta lactone （GDL） as coagulant, we produced light green and very nourishing Undaria pinnatifida bean curd. Using the single factor experiment to determine the quantity and concentration of Undaria pinnatifida juice milk, process conditions of coagulation is determined by three factors three levels experiment, the best formulation of Undaria pinnatifida tofu is determined by test of four factors and three levels, process conditions are： the addition of Undaria pinnatifida is 20%, soya-bean milk concentration is1：4.5, the amount of GDL is0.30%, solidification time is 15min, solidification temperature is 95 ℃.

Clinical efficacy of Chifeng Yingyuan manipulation for migraine and its effects on endothelin and nitric oxide

Objective To observe clinical efficacy of Ch/J：eng Yingyuan manipulation for migraine and its effects on endothelin （ET） and nitric oxide （NO）. Methods One hundred and seventeen cases were randomly divided into a treatment group （59 cases） and a control group （58 cases）. The Chifeng Yingyuan manipulation was applied in the treatment group while oral administration of flunarizine hydrochloride capsules was applied in the control group for consecutive 4 weeks. Before and after the treatment, the clinical efficacy and migraine score in both groups were compared; radioimmunoassay was used to test level of ET while nitrate reductase method was for level of NO. Results Compared between two groups, the total effective rate was 91.5% in the treatment group, which was superior to 70.7% in the control group （P〈0.05）. After the treatment, the migraine scores were both significantly decreased in two groups （P〈0.05, P〈0.01）, which was more obvious in the treatment group （P〈0.05）. The level of ET and NO was significantly changed after treatment （P〈0.05, P〈0.01）. Compared between two groups, the improvement of ET and NO in the treatment group was better than that in the control group （both P〈0.05）. Conclusion The Chifeng Yingyuan manipulation has positive effect on migraine without any adverse reaction, which could maintain cerebrovascular vasomotor function by regulating level of ET and NO in the plasma.