[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomori...[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomorium songaricum RAPD.[Result] The result showed that in the process of Cynomorium songaricum RAPD analysis,the effect of amplification improved significantly and the dosage of Taq enzyme declined by adding BSA.The suitable concentration of BSA used in RAPD of Cynomorium songaricum Rupr was 2 μg/μl.[Conclusion]It is feasible to add BSA to improve the RAPD analysis of plants.This study has provided foundation for the application of BSA in RAPD analysis.展开更多
基金Supported by the Knowledge Innovation Project of Northwest Institute of Plateau Biology of Chinese Academy of Sciences(CXLY-2002-7)~~
文摘[Objective] The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).[Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomorium songaricum RAPD.[Result] The result showed that in the process of Cynomorium songaricum RAPD analysis,the effect of amplification improved significantly and the dosage of Taq enzyme declined by adding BSA.The suitable concentration of BSA used in RAPD of Cynomorium songaricum Rupr was 2 μg/μl.[Conclusion]It is feasible to add BSA to improve the RAPD analysis of plants.This study has provided foundation for the application of BSA in RAPD analysis.
