转BpCUCt白桦生长特征及内源IAA含量的变化

为了揭示白桦Bp CUCt基因的功能,分别构建了Bp CUC2启动子及35S启动子驱动的Bp CUCt载体,以白桦合子胚为受体开展转基因工作,观察转基因株系表型变化,并测定转基因株系和WT的内源激素IAA含量。结果表明,对比转基因株系生长特性可知,转Bp CUCt基因白桦株系比WT矮小,生长也比较缓慢,转基因株系节间距均变长,而Pro CUC2::Bp CUCt转基因株系叶片边缘比WT叶片平滑,35S::Bp CUCt转基因株系叶边缘与野生型并无明显区别。由转基因白桦和WT的内源激素IAA含量可知,83.33%的转基因株系左侧叶片、右侧叶片及主脉IAA含量均显著高于WT株系。表明Bp CUCt基因在一定程度上与Bp CUC2基因表现出相同的功能,同时改变白桦内源生长素IAA的含量。

Study on Variation of Endogenous Hormones at the Germinating Stage of Tea Spring Shoot

An HPLC method was used to analyze the contents and variation of IAA,GA3,ABA.and ZT at five stages around the tea shoot germinating in spring.The contents of GAa and ABA had a top value during the winter and decreased with the growth of tea shoots,while the contents of IAA and ZT had a low value during the winter and increased quickly at the beginning of shoot growth,but soon afterwards increased slowly or decreased a little.The ratio of hormones was closely related to the growth of tea plant.The study indicated that the ratios of GA3 to ABA and IAA to ABA were at low values during the winter and went up with the shoot genninating.When the activity of roots was weak,the ratio of ZT to IAA had a top value,but went down gradually with luxuriant activity of roots.The ratio of GA3 to ZT had a certain relativity with the shoot genninating,which was at a top value during the winter but went down suddenly at the beginning of shoot genninating.