内生拟盘多毛孢菌J63(Pestalotiopsis sp.J63)是从东海的海滩滩涂中新分离出的一株真菌。研究考察了无机盐对该菌株产漆酶响,以及对漆酶催化底物和脱色偶氮类染料甲基橙的影响。结果表明添加H3BO3、SrCl2.6H2ONaF、KBr、MnCl2.4H2O和ZnS...内生拟盘多毛孢菌J63(Pestalotiopsis sp.J63)是从东海的海滩滩涂中新分离出的一株真菌。研究考察了无机盐对该菌株产漆酶响,以及对漆酶催化底物和脱色偶氮类染料甲基橙的影响。结果表明添加H3BO3、SrCl2.6H2ONaF、KBr、MnCl2.4H2O和ZnSO4.7H2O对漆酶的产生有明显的促进作用,其中H3BO3的作用最明显,可使酶活增加221.8%。在漆酶催化底物反应时,除1 mmol L 1FeSO4.7H2O能够抑制漆酶酶活,其余无机盐均对酶活有促进作用。14 mmol L 1KAl(SO4)2对酶活的促进作用明显,使相对酶活达到124.9%。3 mmol L 1KBr使相对酶活达到116.7%,与其他菌株所产漆酶受到KBr抑制的特性相比具有优势。添加14 mmol L 1KAl(SO4)2和3 mmol L 1KBr后相应的脱色率分别达到了97.3%和75.6%,高于对照组64%的脱色率。同时还研究了适宜该菌株产漆酶的温度和pH,当温度为26oC,pH为6.0时酶活最高,达到3280.6 U L 1。展开更多
5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isol...5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4＇-forrnamidophenyl-5-methoxybenzoate （1）, along with seven known polyketides, 4-hydroxybenzoic acid （2）, LL-P880ct （3）, 1＇-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one （4）, pestalotiollide B （5）, pestalotiopyrone G （6）, endocrocin （7） and 2＇-hydroxy- 6＇-hydroxymethyl-4＇-methylphenyl-2,6-dihydroxy-3-（2-isopentenyl）benzoate （8）. HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.展开更多
文摘内生拟盘多毛孢菌J63(Pestalotiopsis sp.J63)是从东海的海滩滩涂中新分离出的一株真菌。研究考察了无机盐对该菌株产漆酶响,以及对漆酶催化底物和脱色偶氮类染料甲基橙的影响。结果表明添加H3BO3、SrCl2.6H2ONaF、KBr、MnCl2.4H2O和ZnSO4.7H2O对漆酶的产生有明显的促进作用,其中H3BO3的作用最明显,可使酶活增加221.8%。在漆酶催化底物反应时,除1 mmol L 1FeSO4.7H2O能够抑制漆酶酶活,其余无机盐均对酶活有促进作用。14 mmol L 1KAl(SO4)2对酶活的促进作用明显,使相对酶活达到124.9%。3 mmol L 1KBr使相对酶活达到116.7%,与其他菌株所产漆酶受到KBr抑制的特性相比具有优势。添加14 mmol L 1KAl(SO4)2和3 mmol L 1KBr后相应的脱色率分别达到了97.3%和75.6%,高于对照组64%的脱色率。同时还研究了适宜该菌株产漆酶的温度和pH,当温度为26oC,pH为6.0时酶活最高,达到3280.6 U L 1。
基金National Natural Science Foundation(Grant No.31660251)Fundamental Research Funds for the Central Universities(Grant No.0903005203401)the Start-up Fund for the“Hundred Young-Talent Scheme”Professorship(Grant No.0236011104424)
文摘5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4＇-forrnamidophenyl-5-methoxybenzoate （1）, along with seven known polyketides, 4-hydroxybenzoic acid （2）, LL-P880ct （3）, 1＇-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one （4）, pestalotiollide B （5）, pestalotiopyrone G （6）, endocrocin （7） and 2＇-hydroxy- 6＇-hydroxymethyl-4＇-methylphenyl-2,6-dihydroxy-3-（2-isopentenyl）benzoate （8）. HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.
