Avicennin A, a new isocoumarin, was isolated from ethyl acetate extract of the mangrove endophytic fungus No.2533 from the South China Sea, its structure was elucidated using spectroscopic methods, primarily 2D NMR te...Avicennin A, a new isocoumarin, was isolated from ethyl acetate extract of the mangrove endophytic fungus No.2533 from the South China Sea, its structure was elucidated using spectroscopic methods, primarily 2D NMR techniques.展开更多
Seven compounds (1–7) were identified from the cultivation of the endophytic fungus Exophiala oligosperma (EN-21) that was isolated from the inner tissue of the marine red alga Laurencia similis. Their structures wer...Seven compounds (1–7) were identified from the cultivation of the endophytic fungus Exophiala oligosperma (EN-21) that was isolated from the inner tissue of the marine red alga Laurencia similis. Their structures were identified with spectroscopic and chemical methods as 2-phenoxynaphthalene (1), (2S, 3R, 4E, 8E)-1-O-β-D-glucopyranosyl-3-hydroxy-2-[(R)-2′-hydroxyoctadecanoyl] amino-9-methyl-4, 8-octadeca-diene (2), (22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol (3), (22E, 24R)-3β, 5α, 9α-trihydroxy- ergosta-7, 22-dien-6-one (4), (22E, 24R)-5α, 6α-epoxy-ergosta-8, 22-dien-3β, 7α-diol (5), (22E, 24R)- ergosta-4, 6, 8(14), 22-tetraen-3-one (6), and euphorbol (7). This paper reports for the first time the chemical constituents of fungus Exophiala oligosperma and the discovery of compound 1 as a natural product from the fungus.展开更多
The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A, a tetranorlabdane diterpenoid active against lung cancer. However, the fermentation yield of as...The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A, a tetranorlabdane diterpenoid active against lung cancer. However, the fermentation yield of asperolide A was very low and only produced in static cultures. Static fermentation conditions of A. wentii EN-48 were optimized employing response surface methodology to enhance the production of asperolide A. The optimized conditions resulted in a 13.9-fold yield enhancement, which matched the predicted value, and the optimized conditions were successfully used in scale-up fermentation for the production of asperolide A. Exogenous addition of plant hormones (especially 10 pmol/L methyl jasmonate) stimulated asperolide A production. To our knowledge, this is first optimized production of an asperolide by a marine-derived fungus. The optimization is effective and valuable to supply material for further anti-tumor mechanism studies and preclinical evaluation of asperolide A and other norditerpenoids.展开更多
文摘Avicennin A, a new isocoumarin, was isolated from ethyl acetate extract of the mangrove endophytic fungus No.2533 from the South China Sea, its structure was elucidated using spectroscopic methods, primarily 2D NMR techniques.
基金Supported by the Programs from the Ministry of Science and Technology of China (Nos.2007AA09Z446,2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences (KZCX2-YW-211-04)
文摘Seven compounds (1–7) were identified from the cultivation of the endophytic fungus Exophiala oligosperma (EN-21) that was isolated from the inner tissue of the marine red alga Laurencia similis. Their structures were identified with spectroscopic and chemical methods as 2-phenoxynaphthalene (1), (2S, 3R, 4E, 8E)-1-O-β-D-glucopyranosyl-3-hydroxy-2-[(R)-2′-hydroxyoctadecanoyl] amino-9-methyl-4, 8-octadeca-diene (2), (22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol (3), (22E, 24R)-3β, 5α, 9α-trihydroxy- ergosta-7, 22-dien-6-one (4), (22E, 24R)-5α, 6α-epoxy-ergosta-8, 22-dien-3β, 7α-diol (5), (22E, 24R)- ergosta-4, 6, 8(14), 22-tetraen-3-one (6), and euphorbol (7). This paper reports for the first time the chemical constituents of fungus Exophiala oligosperma and the discovery of compound 1 as a natural product from the fungus.
基金Supported by the National Natural Science Foundation of China(NSFC,Nos.31200042,31570038)the NSFC-Shandong Joint Fund for Marine Science Research Centers(No.U1406402)
文摘The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A, a tetranorlabdane diterpenoid active against lung cancer. However, the fermentation yield of asperolide A was very low and only produced in static cultures. Static fermentation conditions of A. wentii EN-48 were optimized employing response surface methodology to enhance the production of asperolide A. The optimized conditions resulted in a 13.9-fold yield enhancement, which matched the predicted value, and the optimized conditions were successfully used in scale-up fermentation for the production of asperolide A. Exogenous addition of plant hormones (especially 10 pmol/L methyl jasmonate) stimulated asperolide A production. To our knowledge, this is first optimized production of an asperolide by a marine-derived fungus. The optimization is effective and valuable to supply material for further anti-tumor mechanism studies and preclinical evaluation of asperolide A and other norditerpenoids.