AIM: To investigate the effect of fermented soy milk on human ecosystem in the intestinal tract by way of examining the population of different microorganisms isolated from fecal samples.METHODS: A crossover experimen...AIM: To investigate the effect of fermented soy milk on human ecosystem in the intestinal tract by way of examining the population of different microorganisms isolated from fecal samples.METHODS: A crossover experimental design was applied.Twenty-eight healthy adults completed this experiment.Each subject consumed 250 mL, twice a day between meals, of either fermented soy milk or regular soy milk first for 2 wk, then switched to the other drink after 2 wk.Fecal samples were collected from all subjects every week starting from the second week to the end of the experiment.The microorganisms analyzed were Bifidobacterium spp.,Lactobacillus spp., Clostridium perfringens, coliform organisms, and total anaerobic organisms.RESULTS: In the period of fermented soy milk consumption,the populations of Bifidobacterium spp. and Lactobacillus spp. increased (P＜0.05) as well as the ratios of Bifidobacterium spp. and lactobacillus spp. to Clostridium perfringens (P＜0.05). The population of coliform organisms decreased (P＜0.05) when subjects were in the period of fermented soy milk consumption.CONCLUSION: Intake of fermented soy milk significantly improved the ecosystem of the intestinal tract in the body by increasing the amount of probiotics.展开更多
【背景】微生物在菌根真菌的孢子萌发、菌丝体生长、菌根形成以及子实体发育等过程中起到一定作用。【目的】对采自云南省昆明市嵩明县和楚雄彝族自治州禄丰县的8个干巴菌子实体内的微生物进行分离培养鉴定,为后期研究微生物与干巴菌之...【背景】微生物在菌根真菌的孢子萌发、菌丝体生长、菌根形成以及子实体发育等过程中起到一定作用。【目的】对采自云南省昆明市嵩明县和楚雄彝族自治州禄丰县的8个干巴菌子实体内的微生物进行分离培养鉴定,为后期研究微生物与干巴菌之间的相互作用奠定基础。【方法】采用传统平板分离法从干巴菌子实体内分离获得微生物群落,t检验分析不同地区采集的干巴菌子实体内微生物菌落总数的差异,16S r RNA基因和ITS序列进行系统发育树构建和微生物多样性分析。【结果】采自嵩明县和禄丰县的8个干巴菌子实体内共分离获得282株可培养的细菌,两个地区的细菌菌落总数无显著差异(P=0.22)。所有细菌分属2门12属15种。其中80%的细菌属于变形菌门,且以γ-变形菌为优势菌群,假单胞菌属(Pseudomonas)为优势菌属。其余20%的细菌属于拟杆菌门。从干巴菌子实体中分离获得114株真菌,两个地区的真菌菌落总数无显著差异(P=0.65)。所有真菌分属2门10属10种。其中62%的真菌属于子囊菌门(Ascomycota),并以分离自禄丰县干巴菌子实体内的Lophiostoma为优势属。38%的真菌属于担子菌门(Basidiomycota),并以Asterotremella为优势属。【结论】两个不同地区采集的干巴菌子实体内细菌和真菌在菌落总数上无显著差异。所有细菌都以γ-变形菌为优势菌群,假单胞菌属为优势菌属。嵩明干巴菌子实体内真菌以担子菌门为优势菌群,Asterotremella为优势属。而禄丰干巴菌子实体内真菌则以子囊菌门为优势菌群,Lophiostoma为优势属。展开更多
基金Supported by the Taiwan Tobacco and Liquor Company, No. 0930001444
文摘AIM: To investigate the effect of fermented soy milk on human ecosystem in the intestinal tract by way of examining the population of different microorganisms isolated from fecal samples.METHODS: A crossover experimental design was applied.Twenty-eight healthy adults completed this experiment.Each subject consumed 250 mL, twice a day between meals, of either fermented soy milk or regular soy milk first for 2 wk, then switched to the other drink after 2 wk.Fecal samples were collected from all subjects every week starting from the second week to the end of the experiment.The microorganisms analyzed were Bifidobacterium spp.,Lactobacillus spp., Clostridium perfringens, coliform organisms, and total anaerobic organisms.RESULTS: In the period of fermented soy milk consumption,the populations of Bifidobacterium spp. and Lactobacillus spp. increased (P＜0.05) as well as the ratios of Bifidobacterium spp. and lactobacillus spp. to Clostridium perfringens (P＜0.05). The population of coliform organisms decreased (P＜0.05) when subjects were in the period of fermented soy milk consumption.CONCLUSION: Intake of fermented soy milk significantly improved the ecosystem of the intestinal tract in the body by increasing the amount of probiotics.
文摘【背景】微生物在菌根真菌的孢子萌发、菌丝体生长、菌根形成以及子实体发育等过程中起到一定作用。【目的】对采自云南省昆明市嵩明县和楚雄彝族自治州禄丰县的8个干巴菌子实体内的微生物进行分离培养鉴定,为后期研究微生物与干巴菌之间的相互作用奠定基础。【方法】采用传统平板分离法从干巴菌子实体内分离获得微生物群落,t检验分析不同地区采集的干巴菌子实体内微生物菌落总数的差异,16S r RNA基因和ITS序列进行系统发育树构建和微生物多样性分析。【结果】采自嵩明县和禄丰县的8个干巴菌子实体内共分离获得282株可培养的细菌,两个地区的细菌菌落总数无显著差异(P=0.22)。所有细菌分属2门12属15种。其中80%的细菌属于变形菌门,且以γ-变形菌为优势菌群,假单胞菌属(Pseudomonas)为优势菌属。其余20%的细菌属于拟杆菌门。从干巴菌子实体中分离获得114株真菌,两个地区的真菌菌落总数无显著差异(P=0.65)。所有真菌分属2门10属10种。其中62%的真菌属于子囊菌门(Ascomycota),并以分离自禄丰县干巴菌子实体内的Lophiostoma为优势属。38%的真菌属于担子菌门(Basidiomycota),并以Asterotremella为优势属。【结论】两个不同地区采集的干巴菌子实体内细菌和真菌在菌落总数上无显著差异。所有细菌都以γ-变形菌为优势菌群,假单胞菌属为优势菌属。嵩明干巴菌子实体内真菌以担子菌门为优势菌群,Asterotremella为优势属。而禄丰干巴菌子实体内真菌则以子囊菌门为优势菌群,Lophiostoma为优势属。
