Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem （ES） cells and blocks their differentiation into endoderm. The murine F9 embryonal carcinoma cell line...Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem （ES） cells and blocks their differentiation into endoderm. The murine F9 embryonal carcinoma cell line is a well-documented model system for endoderm cell lineage differentiation. Here, we examined the function of Nanog in F9 cell endoderm differentiation. Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses the differentiation of primitive endoderm and parietal endoderm in F9 cells, whereas it has no effect on the differentiation of visceral endoderm. In contrast, the expression of C-terminal domain-truncated Nanog spontaneously promotes endoderm differentiation in F9 cells. These data suggest that Nanog is required to sustain the proper undifferentiated status of F9 cells, and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietal endoderm differentiation in F9 cells.展开更多
目的探讨子痫前期患者胎盘组织中内皮分化基因2(Edg2)蛋白及m RNA的表达情况及意义。方法选取2015年2月至2016年3月在我院治疗的子痫前期患者46例,其中子痫前期轻度患者27例(轻度组),子痫前期重度患者19例(重度组),同时选取正常晚期妊...目的探讨子痫前期患者胎盘组织中内皮分化基因2(Edg2)蛋白及m RNA的表达情况及意义。方法选取2015年2月至2016年3月在我院治疗的子痫前期患者46例,其中子痫前期轻度患者27例(轻度组),子痫前期重度患者19例(重度组),同时选取正常晚期妊娠妇女25例作为对照组,采用免疫组织化学法和RT-PCR检测各组胎盘组织中Edg2蛋白和m RNA表达水平。结果重度组和轻度组Edg2蛋白阳性表达率分别为84.21%和77.78%,明显高于对照组,差异比较有统计学意义(P﹤0.05);重度组和轻度组Edg2蛋白阳性表达率比较差异无统计学意义(P>0.05);重度组Edg2 m RNA相对表达量为(0.932±0.033),明显高于轻度组和对照组(P﹤0.05);轻度组Edg2 m RNA相对表达量为(0.561±0.036),明显高于对照组(P﹤0.05);Edg2 m RNA相对表达量与疾病程度呈正相关(r=0.893,P﹤0.05)。结论子痫前期患者胎盘组织中Edg2蛋白和m RNA表达明显增高,与疾病严重程度有关。展开更多
文摘Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem （ES） cells and blocks their differentiation into endoderm. The murine F9 embryonal carcinoma cell line is a well-documented model system for endoderm cell lineage differentiation. Here, we examined the function of Nanog in F9 cell endoderm differentiation. Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses the differentiation of primitive endoderm and parietal endoderm in F9 cells, whereas it has no effect on the differentiation of visceral endoderm. In contrast, the expression of C-terminal domain-truncated Nanog spontaneously promotes endoderm differentiation in F9 cells. These data suggest that Nanog is required to sustain the proper undifferentiated status of F9 cells, and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietal endoderm differentiation in F9 cells.
文摘目的探讨子痫前期患者胎盘组织中内皮分化基因2(Edg2)蛋白及m RNA的表达情况及意义。方法选取2015年2月至2016年3月在我院治疗的子痫前期患者46例,其中子痫前期轻度患者27例(轻度组),子痫前期重度患者19例(重度组),同时选取正常晚期妊娠妇女25例作为对照组,采用免疫组织化学法和RT-PCR检测各组胎盘组织中Edg2蛋白和m RNA表达水平。结果重度组和轻度组Edg2蛋白阳性表达率分别为84.21%和77.78%,明显高于对照组,差异比较有统计学意义(P﹤0.05);重度组和轻度组Edg2蛋白阳性表达率比较差异无统计学意义(P>0.05);重度组Edg2 m RNA相对表达量为(0.932±0.033),明显高于轻度组和对照组(P﹤0.05);轻度组Edg2 m RNA相对表达量为(0.561±0.036),明显高于对照组(P﹤0.05);Edg2 m RNA相对表达量与疾病程度呈正相关(r=0.893,P﹤0.05)。结论子痫前期患者胎盘组织中Edg2蛋白和m RNA表达明显增高,与疾病严重程度有关。
