Objective: To observe effects of arginine on arterial endothelium injured by ischemia reperfusion (IR), and explore its possible mechanism. Methods: Fifty four rats were divided into 3 groups and treated in respective...Objective: To observe effects of arginine on arterial endothelium injured by ischemia reperfusion (IR), and explore its possible mechanism. Methods: Fifty four rats were divided into 3 groups and treated in respective ways: (1) drinking tap water as the control; (2) drinking tap water containing 2.5 % L arginine; (3) drinking tap water containing 2.5 % L arginine together with intraperitoneal injection of N G nitro L arginine methylester 5 mg·kg -1 ·d -1 . A segment of the common carotid artery was occluded for 1 h, and then reperfused. Samples taken at different post IR time from the segment were prepared for the ultrastructural and Ce H 2O 2 cytochemical observation. The naked index (NI) of internal elastic lamina (IEL) was measured for comparing the endothelial injure extent and its repair process. Results: Less damage of endothelial cells (EC), more platelets adhering to naked IEL and more regenerating EC were observed in Group 2. The NI values of samples taken at 1, 2, 3 d after the IR were respectively 0.92 ± 0.08 , 0.88 ± 0.03 and 0.41 ± 0.02 in Group 1, and reduced to 0.52 ± 0.05 , 0.19 ± 0.08 and 0.06 ± 0.01 in Group 2 (P< 0.05 0.01 ). In Group 3, the endothelium damage was not alleviated, and so were the NI. The Ce H 2O 2 particles deposited on the lumen surface of endothelium were much less in Group 2 than in Groups 1 and 3. Conclusions: L arginine promotes the repair process of IR injured endothelium probably through the removal of oxygen free radicals by NO.展开更多
文摘Objective: To observe effects of arginine on arterial endothelium injured by ischemia reperfusion (IR), and explore its possible mechanism. Methods: Fifty four rats were divided into 3 groups and treated in respective ways: (1) drinking tap water as the control; (2) drinking tap water containing 2.5 % L arginine; (3) drinking tap water containing 2.5 % L arginine together with intraperitoneal injection of N G nitro L arginine methylester 5 mg·kg -1 ·d -1 . A segment of the common carotid artery was occluded for 1 h, and then reperfused. Samples taken at different post IR time from the segment were prepared for the ultrastructural and Ce H 2O 2 cytochemical observation. The naked index (NI) of internal elastic lamina (IEL) was measured for comparing the endothelial injure extent and its repair process. Results: Less damage of endothelial cells (EC), more platelets adhering to naked IEL and more regenerating EC were observed in Group 2. The NI values of samples taken at 1, 2, 3 d after the IR were respectively 0.92 ± 0.08 , 0.88 ± 0.03 and 0.41 ± 0.02 in Group 1, and reduced to 0.52 ± 0.05 , 0.19 ± 0.08 and 0.06 ± 0.01 in Group 2 (P< 0.05 0.01 ). In Group 3, the endothelium damage was not alleviated, and so were the NI. The Ce H 2O 2 particles deposited on the lumen surface of endothelium were much less in Group 2 than in Groups 1 and 3. Conclusions: L arginine promotes the repair process of IR injured endothelium probably through the removal of oxygen free radicals by NO.
