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小径人工血管内皮化种子细胞的实验研究
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作者 杨震 潘仕荣 +1 位作者 冯炼强 陶军 《中国临床医学》 2004年第2期200-200,共1页
目的:为小径人工血管内皮化种子细胞的实验研究提供理想的种子细胞来源。方法:收集健康成人骨髓,通过Ficoll离心法获得骨髓单个核细胞,置于纤维连接蛋白预衬的DMEM培养基中,用血管内皮生长因子(vascular endothelial growth fac-tor,VE... 目的:为小径人工血管内皮化种子细胞的实验研究提供理想的种子细胞来源。方法:收集健康成人骨髓,通过Ficoll离心法获得骨髓单个核细胞,置于纤维连接蛋白预衬的DMEM培养基中,用血管内皮生长因子(vascular endothelial growth fac-tor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)加以诱导,并通过荧光显微镜和免疫组化分析等方法对诱导后的细胞进行形态学观察和鉴定。结果:在VEGF、bFGF等诱导因子存在的条件下,骨髓单个核细胞分化成为内皮细胞,倒置荧光微镜下呈典形的“纺锤样”梭形细胞,单层细胞贴壁生长融合时呈铺路石样排列,免疫组化示VWF抗体染色阳性,CD34抗体染色阴性。结论:成人骨髓单个核细胞在特定培养条件下可诱导分化成为内皮细胞,可作为小径人工血管内皮化理想的种子细胞。 展开更多
关键词 小径人工血管内皮种子细胞 实验 细胞来源 碱性成纤维细胞生长因子 血管内皮生长因子
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血管基膜衍生多功能肽的表达及其生物活性的鉴定
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作者 戴文建 张曼 +1 位作者 彭淑平 曹建国 《中国肿瘤生物治疗杂志》 CAS CSCD 2006年第6期423-428,共6页
目的:在毕赤酵母中表达血管基膜衍生多功能肽(vascular basement membrane—derived mulifunctional peptide,VB—MDMP),并鉴定其生物学活性。方法:利用PCR技术获得融合了谷胱甘肽转移酶(GST)的肿瘤抑素(tumstatin)活性成分的... 目的:在毕赤酵母中表达血管基膜衍生多功能肽(vascular basement membrane—derived mulifunctional peptide,VB—MDMP),并鉴定其生物学活性。方法:利用PCR技术获得融合了谷胱甘肽转移酶(GST)的肿瘤抑素(tumstatin)活性成分的GST—VBMDMP基因,克隆到pPIC9K载体;然后原生质体转化法转化毕赤酵母细胞,行多克隆筛选和表型鉴定。获得His’Muts表型的转化子,在MGY培养液中30℃摇床培养,每24h从培养液中转移1ml培养液上清于-70℃保存,并保持培养瓶中培养液的量和培养液中甲醇0.5%的浓度不变,第9天行SDS—PAGE检测表达的蛋白并纯化,然后进行细胞和动物生物活性检测。结果:SDS—PAGE发现阳性重组子在MGY培养液中表达的蛋白量在1~7d逐渐增加,到第8天后开始减少,用Gluta—thioneSepharose4B亲和层析柱纯化获得GST—VBMDMP融合蛋白;GST—VBMDMP能抑制C57BL/6鼠动脉内皮细胞管状结构的形成;同时GST—VBMDMP(2、6、10mg/kg)对小鼠Lewis肺癌原发瘤(瘤重抑制率分别为96.6%、82.1%、61.2%)和自发性肺转移瘤(瘤结节抑制率分别为96.8%、87.9%、75、3%)均具有明显的抑制作用(P〈0.01)。结论:VBMDMP能够抑制鼠动脉内皮细胞管状结构的形成,并对小鼠Lewis肺癌原发瘤和肺转移具有明显的抑制作用。 展开更多
关键词 血管基膜衍生多功能肽 抗肿瘤 血管形成抑制剂 血管基底膜 内皮细胞血管化
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肺纤方提取物对肺间质纤维化大鼠肺微血管VEGF、VEGFR2、PAI-1、VCAM-1 mRNA表达的影响 被引量:11
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作者 王晨 王紫娟 +6 位作者 张晓梅 尹婷 刘哲 秦慧慧 张宇婷 顾萧枫 于小林 《北京中医药大学学报》 CAS CSCD 北大核心 2017年第2期143-147,共5页
目的通过观察肺纤方提取物干预体外培养肺微血管的血管内皮细胞生长因子(VEGF)、血管内皮细胞生长因子受体2(VEGFR 2)、纤溶酶原激活物抑制物1(PAI-1)、血管细胞黏附因子1(VCAM-1)基因表达的影响,探讨肺纤方干预肺纤维化微血管生成的机... 目的通过观察肺纤方提取物干预体外培养肺微血管的血管内皮细胞生长因子(VEGF)、血管内皮细胞生长因子受体2(VEGFR 2)、纤溶酶原激活物抑制物1(PAI-1)、血管细胞黏附因子1(VCAM-1)基因表达的影响,探讨肺纤方干预肺纤维化微血管生成的机制。方法从肺纤维化模型大鼠分离培养肺微血管内皮细胞,将其等量分为模型组,氯沙坦组,泼尼松组,肺纤方大、中、小剂量组。模型组加入含20%胎牛血清的DMEM培养基,氯沙坦组加入含20%胎牛血清及10 mg/L氯沙坦的DMEM培养基,泼尼松组加入含20%胎牛血清及5 mg/L泼尼松的DMEM培养基,肺纤方大、中、小剂量组分别加入含20%胎牛血清及100、60、20 mg/L肺纤方提取物的DMEM培养基,分别作用24 h。双链嵌合荧光染料SYBR GreenⅠ实时荧光定量PCR法检测VEGF、VEGFR 2、PAI-1与VCAM-1的mRNA转录水平。结果与模型组比较,肺纤方大、中、小剂量组VEGF基因表达水平均显著降低,而VEGFR2无显著差异;肺纤方大剂量组PAI-1 mRNA表达显著降低;肺纤方大、中剂量组VCAM-1 mRNA表达显著降低(P<0.05)。结论肺纤方可通过抑制肺微血管VEGF、PAI-1及VCAM-1的表达改善血凝及纤溶,抑制血管形成而发挥抗肺纤维化作用。 展开更多
关键词 肺纤方提取物 肺间质纤维血管内皮细胞 血管内皮细胞生长因子 血管内皮细胞生长因子受体2 纤溶酶原激活物抑制物1 血管细胞黏附因子1 大鼠
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Effect of arsenic trioxide on vascular endothelial cell proliferation and expression of vascular endothelial growth factor receptors Flt-1 and KDR in gastric cancer in nude mice 被引量:28
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作者 Yan-Feng Xiao De-Dong Wu +2 位作者 Shan-Xi Liu Xi Chen Li-Fen Ren 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第48期6498-6505,共8页
AIM: To investigate the effect of arsenic trioxide (As2O3) on expression of vascular endothelial growth factor receptor-1 (VEGFR-1, Flt-1) and VEGFR-2 (KDR) in human gastric tumor cells and proliferation of vas... AIM: To investigate the effect of arsenic trioxide (As2O3) on expression of vascular endothelial growth factor receptor-1 (VEGFR-1, Flt-1) and VEGFR-2 (KDR) in human gastric tumor cells and proliferation of vascular endothelial cells.METHODS: The solid tumor model was formed in nude mice with the gastric cancer cell line SGC-7901. The animals were treated with As2O3. Microvessel density (MVD) and expression of Flt-1 and KDR were detected by immunofluorescence laser confocal microscopy. SGC-7901 cells were treated respectively by exogenous recombinant human VEGF165 or VEGF165 + As2O3. Cell viability was measured by MTT assay. Cell viability of ECV304 cells was measured by MTT assay, and cell cycle and apoptosis were analyzed using flow cytometry.RESULTS: The tumor growth inhibition was 30.33% and 50.85%, respectively, in mice treated with As2O3 2.5 and 5 mg/kg. MVD was significantly lower in arsenic-treated mice than in the control group. The fluorescence intensity levels of Flt-1 and KDR were significantly less in the arsenic-treated mice than in the control group. VEGF165 may accelerate growth of SGC7901 cells, but As2O3 may disturb the stimulating effect of VEGF165. ECV304 cell growth was suppressed by 76.51%, 71.09% and 61.49% after 48 h treatment with As2O3 at 0.5, 2.5 and 5 μmol/L, respectively. Early apoptosis in the As2O3- treated mice was 2.88-5.1 times higher than that in the controls, and late apoptosis was 1.17-1.67 times higher than that in the controls.CONCLUSION: Our results showed that As2O3 delays tumor growth, inhibits MVD, down-regulates Flt-1 and KDR expression, and disturbs the stimulating effect of VEGF165 on the growth of SGC7901 cells. These results suggest that As2O3 might delay growth of gastric tumors through inhibiting the paracrine and autocrine pathways of VEGF/VEGFRs. 展开更多
关键词 Arsenic trioxide Gastric tumor Fit-1 Tumor growth inhibition
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Immunohistochemical assessment of angiogenesis in hepatocellular carcinoma and surrounding cirrhotic liver tissues 被引量:19
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作者 GeertuDeli Can-HaoJin +4 位作者 RongMu SongYang YueLiang DeChen MasatoshiMakuuchi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第7期960-963,共4页
AIM: To investigate whether vascular endothelial growth factor (VEGF) was over-expressed in hepatocellular carcinoma (HCC) or in surrounding cirrhotic liver tissues.METHODS: Immunohistochemistry was performed to inves... AIM: To investigate whether vascular endothelial growth factor (VEGF) was over-expressed in hepatocellular carcinoma (HCC) or in surrounding cirrhotic liver tissues.METHODS: Immunohistochemistry was performed to investigate the expression of VEGF proteins in HCC tissues from 105 consecutive patients undergoing curative resection for HCC. The immunostaining results and related clinicopathologic materials were analyzed with statistical methods. Kaplan-Meier method was used to calculate survival curves, and Log-rank test was performed to compare differences in survival rates of the patients with positive HCC staining and negative VEGF.RESULTS: VEGF-positive expression was found in 72 of105 HCC patients (68.6%). Capsular infiltration (P= 0.005),vascular invasion (P = 0.035) and intrahepatic metastasis(P=0.008) were observed more frequently in patients with VEGF-positive expression than in those with VEGFnegative expression. Kaplan-Meier curves showed that VEGF-positive expression was associated with a shorter overall survival (P = 0.014). VEGF-positive expression was found in 47 of tissues 68 HCC (69.1%), and VEGF-positive expression was found in 54 of 68 surrounding cirrhotic liver tissues (79.4%). VEGF-positive expression was significantly higher in surrounding cirrhotic liver tissues than in HCC (P= 0.017).CONCLUSION: VEGF may play an important role in the angiogenesis and prognosis of HCC, as well as in the angiogenesis of liver cirrhosis. 展开更多
关键词 ANGIOGENESIS Vascular endothelial growth factor Hepatocellular carcinoma Surrounding cirrhotic liver tissues
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Adrenomedullin in cirrhotic and non-cirrhotic portal hypertension 被引量:5
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作者 V Tahan E Avsar +9 位作者 C Karaca E Uslu F Eren S Aydin H Uzun HO Hamzaoglu F Besisik C Kalayci A Okten N Tozun 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第10期2325-2327,共3页
AIM:Adrenomedullin (ADM) is a potent vasodilator peptide. ADM and nitric oxide (NO) are produced in vascular endothelial cells.Increased ADM level has been linked to hyperdynamic circulation and arterial vasodilatatio... AIM:Adrenomedullin (ADM) is a potent vasodilator peptide. ADM and nitric oxide (NO) are produced in vascular endothelial cells.Increased ADM level has been linked to hyperdynamic circulation and arterial vasodilatation in cirrhotic portal hypertension (CPH).The role of ADM in non-cirrhotic portal hypertension (NCPH) is unknown,plasma ADM levels were studied in patients with NCPH,compensated and decompensated cirrhosis in order to determine its contribution to portal hypertension (PH) in these groups. METHODS:There were 4 groups of subjects.Group 1 consisted of 27 patients (F/M:12/15) with NCPH due to portal and/or splenic vein thrombosis (mean age:41±12 years),group 2 consisted of 14 patients (F/M:6/8) with compensated (Child-Pugh A) cirrhosis (mean age:46±4), group 3 consisted of 16 patients (F/M:6/10) with decompensated (Child-Pugh C) cirrhosis (mean age:47±12). Fourteen healthy subjects (F/M:6/8) (mean age:44±8) were used as controls in Group 4.ADM level was measured by ELISA.NO was determined as nitrite/nitrate level by chemoluminescence. RESULTS:ADM level in Group 1 (236±61.4 pg/mL) was significantly higher than that in group 2 (108.4±28.3 pg/mL) and group 4 (84.1±31.5 pg/mL) (both P<0.0001) but was lower than that in Group3 (324±93.7 pg/mL) (P=0.002).NO level in group 1 (27±1.4 μmol/L) was significantly higher than that in group 2 (19.8±2.8 μmol/L) and group 4 (16.9±1.6 μmol/L) but was lower than that in Group 3 (39±3.6 μmol/L) (for all three P<0.0001).A strong correlation was observed between ADM and NO levels (r=0.827,P<0.0001). CONCLUSION:Adrenomedullin and NO levels were high in both non-cirrhotic and cirrhotic portal hypertension and were closely correlated,Adrenomedullin and NO levels increased proportionally with the severity of cirrhosis,and were significantly higher than those in patients with NCPH. Portal hypertension plays an important role in the increase of ADM and NO.Parenchymal damage in cirrhosis may contribute to the increase in these parameters. 展开更多
关键词 Adult Comparative Study Female Humans Hypertension Portal Liver Cirrhosis Male Middle Aged NITRATES Nitric Oxide NITRITES PEPTIDES VASODILATION
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Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells 被引量:1
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作者 Károly Palatka Zoltán Serf(o|″)z(o|″) +7 位作者 Zoltán Veréb Róbert Bátori Beáta Lontay Zoltán Hargitay Zoltán Nemes Miklós Udvardy Ferenc Erd(o|″)di István Altorjay 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第11期1730-1738,共9页
AIM: To study the expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) and their role in inflammatory bowel disease (IBD). METHODS: We examined the effect of sera obtained from patients... AIM: To study the expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) and their role in inflammatory bowel disease (IBD). METHODS: We examined the effect of sera obtained from patients with active Crohn's disease (CD) and ulcerative colitis (UC) on the function and viability of human umbilical vein endothelial cells (HUVEC). HUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the NOS inhibitor, 10^-4 mol/L L-NAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. RESULTS: In HUVEC immediately after exposure to UC, serum eNOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with CD sera and the eNOS level was minimal at 20 h compared to control (18%±16% vs 23%± 15% P〈0.01). UC or CD serum caused a significant increase in iNOS compared to control (UC: 300%±21%; CD: 275% ± 27% vs 108% ± 14%, P〈0.01). Apoptosis/necrosis characteristics did not differ significantly in either experiment. Increased proliferation activity was detected in the presence of CD serum or after treatment with L-NAME. Cultures showed tube-like formations after 24 h treatment with CD serum. CONCLUSION: IBD sera evoked changes in the ratio of eNOS/iNOS, whereas did not influence the viability of HUVEC. These involved down-regulation of eNOS and up-regulation of iNOS simultaneously, leading to increased proliferation activity and possibly a reduced antiinflammatory protection of endothelial cells. 展开更多
关键词 Crohn's disease Human umbilical vein endothelial cells Inflammatory bowel disease Nitric oxide synthase Ulcerative colitis
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C-reactive protein,procalcitonin,interleukin-6,vascular endothelial growth factor and oxidative metabolites in diagnosis of infection and staging in patients with gastric cancer 被引量:18
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作者 NevinIlhan NecipIlhan +2 位作者 MehmetKcksu Yavuz Ilhan Handan Akbulut 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第8期1115-1120,共6页
AIM:The current study was to determine the serum/pLasma levels of VEGF,IL-6,malondialdehyde (MDA),nitric oxide (NO),PCT and CRP in gastric carcinoma and correlation with the stages of the disease and accompanying infe... AIM:The current study was to determine the serum/pLasma levels of VEGF,IL-6,malondialdehyde (MDA),nitric oxide (NO),PCT and CRP in gastric carcinoma and correlation with the stages of the disease and accompanying infection. METHODS:We examined the levels of serum VEGF,IL-6, PCT,CRP and plasma MDA,NO in 42 preoperative gastric cancer patients and 23 healthy subjects.There were infection anamneses that had no definite origin in 19 cancer patients. RESULTS:The VEGF levels (mean±SD; pg/mL) were 478.05±178.29 and 473.85±131.24 in gastric cancer patients with and without infection,respectively,and these values were not significantly different (P>0.05).The levels of VEGF, CRP,PCT,It-6,MDA and NO in cancer patients were significantly higher than those in healthy controls and the levels of CRP,PCT,It-6,MDA and NO were statistically increased in infection group when compared with non- infection group (P<0.001). CONCLUSION:Although serum VEGF concentrations were increased in gastric cancer,this increase might not be related to infection.CRP,PCT,IL-6,MDA and NO have obvious drawbacks in the diagnosis of infections in cancer patients. These markers may not help to identify infections in the primary evaluation of cancer patients and hence to avoid unnecessary antibiotic treatments as well as hospitalization. According to the results of this study,IL-6,MDA,NO and especially VEGF can be used as useful parameters to diagnose and grade gastric cancer. 展开更多
关键词 Abdominal Pain Aged Biological Markers C-Reactive Protein CALCITONIN Female Helicobacter Infections Humans INTERLEUKIN-6 Male MALNUTRITION MALONDIALDEHYDE Middle Aged Neoplasm Staging Nitric Oxide Oxidative Stress Protein Precursors Stomach Neoplasms Tumor Markers Biological Vascular Endothelial Growth Factor A VOMITING Weight Loss
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VEGF in hepatocellular carcinoma and surrounding cirrhotic liver tissues 被引量:5
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作者 Muriel Mathonnet Bernard Descottes +2 位作者 Denis Valleix Francois Labrousse Yves Denizot 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第5期830-831,共2页
We read with a great interest the recent work of Deli and colleagues. in the World Journal of Gastroenterology reporting vascular endothelial growth factor (VEGF) expression in hepatocellular carcinoma (HCC) and c... We read with a great interest the recent work of Deli and colleagues. in the World Journal of Gastroenterology reporting vascular endothelial growth factor (VEGF) expression in hepatocellular carcinoma (HCC) and cirrhotic liver tissues. This well-documented work shows that VEGF was significantly higher in surrounding cirrhotic liver tissues than in HCC. Authors assessed VEGF expression using immunohistochemistry. The immunohistochemical staining is an efficient tool to assess the percentage of cells stained positively for VEGF but is not really efficient to estimate their true VEGF content. Evaluation of the VEGF protein by an enzyme-linked immunosorbent assay 0ELISA) has been reported, by us and others, to be an efficient tool in order to assess tissue VEGF expression. We have, thus, tested whether the ELISA method might be an efficient tool in order to confirm data reporting higher amounts of VEGF in surrounding cirrhotic liver tissues than in HCC. Deli and colleagues. also correctly pointed out that basic fibroblast growth factor (bFGF) has been reported to act cooperatively on VEGF expression. We have, thus, also assessed bFGF tissue levels in order to search for a putative link between VEGF and bFGF levels in cirrhotic tissues. 展开更多
关键词 Vascular endothelial growth factor Hepatocellular carcinoma Cirrhotic liver tissues
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Effect of intraarterial chemotherapy on vascular endothelial growth factor expression and microvessel density in carcinoma of the cervix 被引量:5
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作者 Yongxiu Qiu Ghunlin Ghen +1 位作者 Ping Liu Yili Wei 《The Chinese-German Journal of Clinical Oncology》 CAS 2007年第1期68-71,共4页
Objective: To clarify the effect of intraarterial chemotherapy on vascular endothelial growth factor (VEGF) expres- sion and microvessel density (MVD) count in carcinoma of the cervix. Methods: Before intraarterial ch... Objective: To clarify the effect of intraarterial chemotherapy on vascular endothelial growth factor (VEGF) expres- sion and microvessel density (MVD) count in carcinoma of the cervix. Methods: Before intraarterial chemotherapy and after 2–3 weeks of therapy, the expression of VEGF and MVD count in 36 carcinoma tissues of locally advanced cervical cancer were determined by CD34. Results: Before intraarterial chemotherapy and after 2–3 weeks, the expression of VEGF were 75% (27/36) and 30.6% (11/36) respectively, and MVD were reduced obviously (P<0.001). Conclusion:?The intraarterial chemotherapy can reduce the expression of VEGF and MVD, and adjust malignancy of cervical cancer, and cut down the postoperative metastasis. 展开更多
关键词 cervical cancer intraarterial chemotherapy vascular endothelial growth factor microvessel density
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Continued application of Endostar combined with chemotherapy in advanced hemangioendothelioma of bone
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作者 Ningrong Yang Lin Wang Xun Chen Shukui Qin 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第11期672-675,共4页
By one case of hemangioendothelioma of bone accompanying pulmonary metastasis was treated with rh-endostatin injection (Endostar) combined with chemotherapy. The patient got partial response (PR) for 3 years after the... By one case of hemangioendothelioma of bone accompanying pulmonary metastasis was treated with rh-endostatin injection (Endostar) combined with chemotherapy. The patient got partial response (PR) for 3 years after the application of Endostar maintenance therapy and Endostar combined with taxane-based chemotherapy. During the period of using Endostar as monotherapy, the patient got long-term disease control and good quality of life. There was no drug related adverse event during the therapy of Endostar. Suggested continued using of Endostar combined with chemotherapy could achieve an convinced therapeutic effect. Then using Endostar as maintenance treatment after patient got the optimal efficacy was feasible and profitable. This treatment strategy of long-term administration of Endostar was worthy of further observation, to explore the feasibility for long-term administration of combined with chemotherapy in the treatment of hemangioendothelioma of bone accompanying pulmonary metastasis. 展开更多
关键词 hemangioendothelioma of bone rh-endostar/Endostar ANTI-ANGIOGENESIS maintenance therapy
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Protection of Salvianolic Acid B for Human Endothelial Cells Against Hydrogen Peroxide-Induced Oxidative Damage
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作者 张俊刚 赵广荣 +1 位作者 刘金玲 季祥武 《Transactions of Tianjin University》 EI CAS 2009年第6期434-439,共6页
Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was ... Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured. Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glu-tathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glu-tathione concentration after the oxidative challenge. 展开更多
关键词 salvianolic acid B redox status human endothelial cell hydrogen peroxide oxidative stress antioxidant enzyme reductive glutathione
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Role of p38 Mitogen-activated Protein Kinase in Mediating Monocyte Chemoattractant Protein-1 in Human Umbilical Vein Endothelial Cells
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作者 李艳波 邓华聪 +1 位作者 郑丹 李呼伦 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第1期71-71,共1页
关键词 Cells Cultured Endothelial Cells Humans Mitogen-Activated Protein Kinases Monocyte Chemoattractant Protein-1 RNA Messenger Research Support Non-U.S. Gov't Umbilical Veins p38 Mitogen-Activated Protein Kinases
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Methoxyl methyl ether isoamylene quercetin, a quercetin derivative, protects rat aorta endothelial cells against oxidation and apoptosis
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作者 刘秀梅 刘晓岩 王银叶 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2013年第4期355-360,共6页
Methoxyl methyl ether isoamylene quercetin (MIAQ) is one of the newly synthesized quercetin derivatives. The present study investigated the effect of MIAQ on rat aorta endothelial cells (RAECs) injured by hydrogen... Methoxyl methyl ether isoamylene quercetin (MIAQ) is one of the newly synthesized quercetin derivatives. The present study investigated the effect of MIAQ on rat aorta endothelial cells (RAECs) injured by hydrogen peroxide (H2O2), as well as the potential mechanisms. We observed that MIAQ at 2.5-10μmol/L significantly enhanced the viability of injured RAECs, and the effect was more potent than quercetin and ct-tocopherol. However, M1AQ at the same concentration failed to show anti-oxidant activity in a cell-free system. In H2O2-injured endothelial cells treated with MIAQ (5-10μmol/L), the level of nitric oxide (NO) and malondialdehyde was decreased, and the activities of superoxide dismutase and glutathione peroxidase was enhanced. In addition, RAECs treated with MIAQ (2.5-10 μmol/L) exhibited significant inhibiting apoptosis. In conclusion, MIAQ had protective effect on RAECs, possibly through increasing NO production and antioxidases activities, as well as inhibiting apoptosis. These findings suggest that MIAQ is possibly beneficial in the prevention of atherosclerosis and other diseases related to endothelial injury. 展开更多
关键词 Methoxyl methyl ether isoamylene quercetin Rat aorta endothelial cells Nitric oxide ANTI-OXIDATION ANTI-APOPTOSIS
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An updated view on the differentiation of stem cells into endothelial cells 被引量:3
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作者 ZHOU YiJiang YANG Feng +4 位作者 CHEN Ting WU YuTao YANG Mei ZHU JianHua ZHANG Li 《Science China(Life Sciences)》 SCIE CAS 2014年第8期763-773,共11页
Endothelial cells form the internal barrier between circulating blood and the vessel wall.They regulate arterial activity and mediate pathological reactions to vascular injuries such as atherosclerosis and balloon ang... Endothelial cells form the internal barrier between circulating blood and the vessel wall.They regulate arterial activity and mediate pathological reactions to vascular injuries such as atherosclerosis and balloon angioplasty.The development and differentiation of endothelial cells is a complex and coordinated process involving multiple levels of signaling and transcriptional and post-transcriptional regulation.Elucidating the mechanism of endothelial differentiation will not only enhance our understanding of vascular disease pathogenesis,but also facilitate our ability to produce vessels cells from pluripotent stem cells for regeneration purposes.In this review,we discuss the current understanding of how stem cells differentiate into endothelial cells at the level of signaling,transcription and microRNA regulation. 展开更多
关键词 stem cells endothelial cells DIFFERENTIATION SIGNALING TRANSCRIPTION MICRORNA
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