Endophytic fungus is a rich source of bioactive metabolites with multiple activities.In the present study,eight endophytic fungal strains(Z-1 to Z-8)from Zanthoxylum armatum DC.were isolated and identified,and the ant...Endophytic fungus is a rich source of bioactive metabolites with multiple activities.In the present study,eight endophytic fungal strains(Z-1 to Z-8)from Zanthoxylum armatum DC.were isolated and identified,and the anti-inflammatory effect of their extracts was evaluated in vitro.The results showed that four endophytic fungal extracts(Fusarium oxysporum Schlecht.(Z-1),Alternaria arborescens E.G Simmons(Z-3),Mucor fragilis Bainier(Z-4)and Aspergillus terreus Thom(Z-6))displayed good activities in inhibition of excessive production of nitric oxide in lipopolysaccharide-activated mouse macrophage RAW264.7 cells.Among them,the extracts of Fusarium oxysporum Schlecht showed the maximum inhibition activity with a half maximal inhibitory concentration of 174.0μg/m L.The underlying mechanism of the anti-inflammatory effect of Fusarium oxysporum Schlecht extract was associated with its inhibition on the release of several inflammatory factors.The m RNA expression of interleukin 1β,interleukin 6,inducible nitric oxide synthase in lipopolysaccharide-activated mouse macrophage RAW264.7 cells were all inhibited by Z-1 extract detected by real-time polymerase chain reaction.In summary,several endophytic fungal from Zanthoxylum armatum have good anti-inflammatory activity and Fusarium oxysporum Schlecht possesses a promising potential to be developed to a novel anti-inflammatory agent.展开更多
基金The Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province(Grant No.20180807)Undergraduate innovation and entrepreneurship training program of Lanzhou University of Technology(Grant No.DC2020249)。
文摘Endophytic fungus is a rich source of bioactive metabolites with multiple activities.In the present study,eight endophytic fungal strains(Z-1 to Z-8)from Zanthoxylum armatum DC.were isolated and identified,and the anti-inflammatory effect of their extracts was evaluated in vitro.The results showed that four endophytic fungal extracts(Fusarium oxysporum Schlecht.(Z-1),Alternaria arborescens E.G Simmons(Z-3),Mucor fragilis Bainier(Z-4)and Aspergillus terreus Thom(Z-6))displayed good activities in inhibition of excessive production of nitric oxide in lipopolysaccharide-activated mouse macrophage RAW264.7 cells.Among them,the extracts of Fusarium oxysporum Schlecht showed the maximum inhibition activity with a half maximal inhibitory concentration of 174.0μg/m L.The underlying mechanism of the anti-inflammatory effect of Fusarium oxysporum Schlecht extract was associated with its inhibition on the release of several inflammatory factors.The m RNA expression of interleukin 1β,interleukin 6,inducible nitric oxide synthase in lipopolysaccharide-activated mouse macrophage RAW264.7 cells were all inhibited by Z-1 extract detected by real-time polymerase chain reaction.In summary,several endophytic fungal from Zanthoxylum armatum have good anti-inflammatory activity and Fusarium oxysporum Schlecht possesses a promising potential to be developed to a novel anti-inflammatory agent.
