缬沙坦对高血压左室肥厚及外周淋巴细胞碱化的影响

目的:探讨缬沙坦对高血压左室肥厚及心肌细胞内碱化的影响。方法:采用缬沙坦与咪哒普利两组治疗,观察两组高血压病人治疗前左室质量指数(LV M I)、细胞内pH变化并进行了比较。结果:缬沙坦与咪哒普利组皆能减少LV M I、pH值,两组间比较P>0.05,治疗前后比较P<0.05。结论:缬沙坦能明显减少高血压左室肥厚的LV M I及心肌细胞内的pH值,纠正细胞内碱化,逆转左心室肥厚(LV H)。

NHE—1的表达、调控与白血病细胞多药耐药

多药耐药(MDR)机制多种多样且往往复合存在。目前发现MDR细胞pHi均高于敏感细胞,而Na^+-H^+交换蛋白-1(NHE-1)是调节pHi的主要离子通道蛋白,故通过抑制NHE-1降低MDR细胞pHi可能成为化疗增敏、逆转MDR的新手段。

Biochemical response of the mussel Mytilus coruscus(Mytiloida:Mytilidae) exposed to in vivo sub-lethal copper concentrations

Many aquatic organisms are negatively affected by exposure to high copper concentrations. We investigated the biochemical response of the mussel Mytilus coruscus （Mytiloida： Mytilidae） to copper exposure. In vivo bioassays using M. coruscus and different copper concentrations were conducted. The activity of six biomarkers, namely superoxide dismutase （SOD）, catalase （CAT）, acid phosphatase （ACP）, alkaline phosphatase （AKP）, glutamic-oxaloacetic transaminase （GOT） and glutamic-pyruvic transaminase （GPT） were measured. Survival rates decreased with increased copper concentrations and exposure times. The LCs0 values at 48, 72, and 96 h exposure were 0.48, 0.37, and 0.32 rag/L, respectively. Within digestive glands, CAT activity increased with increasing Cu concentrations. The activity of AKP showed no significant change, while the remaining four enzymes showed decreasing activity with increasing Cu concentrations. Within the gills, AKP activity increased when the Cu concentration was 0.05 mg/L, but showed no significant changes at higher concentrations. Activity of CAT and ACP within gills tended to decrease with increasing Cu concentration. The activity of SOD and GPT decreased at an exposure concentration of 0.2 mg/L. GOT activity within gills decreased at 0.1 mg/L and increased at an exposure concentration of 0.2 mg/L. Within the adductor muscle, AKP activity increased at 0.05 mg/L but did not change at higher exposure concentrations. ACP activity within adductor muscle tissue showed no change, while activities of CAT, GOT and GPT decreased with increasing Cu concentrations. SOD activity within the adductor muscle tissue significantly decreased at the 0.02, 0.05 and 0.2 mg/L exposure concentrations. Our results show tissue specific differences for the six biomarkers in for M. coruscus. Our findings provide the basis for the establishment of reference activity levels against which biomarker changes can be estimated, and are essential preliminary steps in development of in vivo bioassays.

Unexpected anterograde amnesia associated with Buscopan used as a predmedication for endocscopy

It has been known that peripheral adverse event is caused by peripheral antimuscarinic action, from hyoscine butylbromide (Buscopan; Boehringer Ingelheim, Germany) used as a premedication for endoscopy. However, symptoms or signs associated with the central nervous system are rarely reported in the field of anesthesiology and peripartum labor. This central anticholinergic syndrome is likely caused by blockade of muscarinic cholinergic receptors in the central nervous system. There is no report on Buscopan-induced central anticholinergic syndrome in endoscopy room so far. Three middle-aged females unexpectedly suffered from anterograde amnesia after intramuscular injection of hyoscine butylbromide as an antispasmodic premedication for endoscopy at our endoscopy unit in the Health Promotion Center.

逆转肿瘤微环境异常pH梯度的抗癌新策略

外酸化与内碱化的现象普遍存在于不同类型的恶性肿瘤中。这种微环境异常pH梯度的形成与肿瘤细胞的组织结构和代谢特点密切相关。微环境负pH梯度的维持与一些离子交换蛋白,如Na+/H+交换体-1(NHE-1)、空泡质子泵(V-ATPase)、HCO3-转运体等息息相关。碳酸酐酶(CA)对肿瘤细胞内外pH梯度的维持也有着重要意义。综述肿瘤微环境失调pH的成因、异常的微环境pH对肿瘤生长发展的影响以及一些逆转微环境异常pH梯度的治疗策略,为相关药物的开发提供思路。