Objective：To evaluate and compare electroacupunctures（EA） with different parameters and moxibustion at different temperatures influencing the activation of mast cells（MC） in Tianshu（ST 25） regions of visceral h...Objective：To evaluate and compare electroacupunctures（EA） with different parameters and moxibustion at different temperatures influencing the activation of mast cells（MC） in Tianshu（ST 25） regions of visceral hyperalgesia model rats.Methods：Rats（except for model group） respectively accepted 1 m A or 3 m A EA or moxibustion at 43 or 4 to ℃ ℃stimulate Tianshu（ST 25） points after randomization of the fifty visceral hyperalgesia model rats,and then were compared with that in model and normal groups.Number,degranulation numbers,degranulation rates in Tianshu（ST 25） regions MC of rats in each group were observed using toluidine blue staining.Abdominal withdrawl reflex（AWR） score was used to evaluate the rat visceral hyperalgesia reactions.Results：Compared with the normal group and the model group,MC numbers（P〈0.05,P〈0.01,P〈0.01,P〈0.01）,degranulation numbers and degranulation rates（P〈0.01,P〈0.01,P〈0.05,P〈0.01） of Tianshu（ST 25） MC in regions tissues in 43 and 4 moxibustion groups,and 1 m A and 3 m℃ ℃A EA groups all increased significantly.Compared with the model group,AWR scores were significantly lower in 43 and 4 ℃ moxibustion groups,and 1 m A and 3 m℃A EA groups under the stimulation of 20 mm Hg,40 mm Hg,0 mm Hg or 80 mm Hg colorectal distension（CRD）（P〈0.05 in 1 m A and 3 m A EA groups under the stimulation of 20 mm Hg,P〈0.01 in the other groups）.AWR scores in 43 ℃and 4 ℃moxibustion groups under the stimulation of 20 mm Hg,40 mm Hg,0 mm Hg or 80 mm Hg CRD were not significantly different from those in the normal group（all P〈0.05）;AWR scores in 1 m A EA group under the stimulation of 0 mm Hg or 80 mm Hg were significantly higher than that in the normal group（P〈0.01）;AWR score in 3 m A EA group under the stimulation of 0 mm Hg was significantly higher than that in the normal group（P〈0.01）,and AWR scores in 3 m A EA group under the stimulation of 20 mm Hg or 80 mm Hg were also higher than that in the normal group（P〈0.05）.AWR scores were higher in 1 m A EA group under the stimulation of 40 mm Hg or 80 mm Hg than that in 4 moxibustion group（℃ P〈0.05）;AWR score was higher in 3 m A EA group under the stimulation of 40 mm Hg than that in 4 moxibustion group（℃ P〈0.05）.Conclusion：There are differences among EA of different parameters and moxibustion of different temperatures in activating on Tianshu（ST 25） regions MC of visceral hyperalgesia model rats,as well as in improving the visceral hyperalgesia reaction.The effect of 4 moxibustion℃ is the most significant.展开更多
基金supported by National Basic Research Program of China(973 Program,No.2015CB554500)National Natural Science Foundation of China(No.30973784)~~
文摘Objective：To evaluate and compare electroacupunctures（EA） with different parameters and moxibustion at different temperatures influencing the activation of mast cells（MC） in Tianshu（ST 25） regions of visceral hyperalgesia model rats.Methods：Rats（except for model group） respectively accepted 1 m A or 3 m A EA or moxibustion at 43 or 4 to ℃ ℃stimulate Tianshu（ST 25） points after randomization of the fifty visceral hyperalgesia model rats,and then were compared with that in model and normal groups.Number,degranulation numbers,degranulation rates in Tianshu（ST 25） regions MC of rats in each group were observed using toluidine blue staining.Abdominal withdrawl reflex（AWR） score was used to evaluate the rat visceral hyperalgesia reactions.Results：Compared with the normal group and the model group,MC numbers（P〈0.05,P〈0.01,P〈0.01,P〈0.01）,degranulation numbers and degranulation rates（P〈0.01,P〈0.01,P〈0.05,P〈0.01） of Tianshu（ST 25） MC in regions tissues in 43 and 4 moxibustion groups,and 1 m A and 3 m℃ ℃A EA groups all increased significantly.Compared with the model group,AWR scores were significantly lower in 43 and 4 ℃ moxibustion groups,and 1 m A and 3 m℃A EA groups under the stimulation of 20 mm Hg,40 mm Hg,0 mm Hg or 80 mm Hg colorectal distension（CRD）（P〈0.05 in 1 m A and 3 m A EA groups under the stimulation of 20 mm Hg,P〈0.01 in the other groups）.AWR scores in 43 ℃and 4 ℃moxibustion groups under the stimulation of 20 mm Hg,40 mm Hg,0 mm Hg or 80 mm Hg CRD were not significantly different from those in the normal group（all P〈0.05）;AWR scores in 1 m A EA group under the stimulation of 0 mm Hg or 80 mm Hg were significantly higher than that in the normal group（P〈0.01）;AWR score in 3 m A EA group under the stimulation of 0 mm Hg was significantly higher than that in the normal group（P〈0.01）,and AWR scores in 3 m A EA group under the stimulation of 20 mm Hg or 80 mm Hg were also higher than that in the normal group（P〈0.05）.AWR scores were higher in 1 m A EA group under the stimulation of 40 mm Hg or 80 mm Hg than that in 4 moxibustion group（℃ P〈0.05）;AWR score was higher in 3 m A EA group under the stimulation of 40 mm Hg than that in 4 moxibustion group（℃ P〈0.05）.Conclusion：There are differences among EA of different parameters and moxibustion of different temperatures in activating on Tianshu（ST 25） regions MC of visceral hyperalgesia model rats,as well as in improving the visceral hyperalgesia reaction.The effect of 4 moxibustion℃ is the most significant.
