[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant D...[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.展开更多
Objective： This study aimed to evaluate of the accuracy and efficiency of the in-vivo dosimetry systems for routine cancer patient dose verification. Methods： In vivo dosimetry, using diodes and thermoluminescent do...Objective： This study aimed to evaluate of the accuracy and efficiency of the in-vivo dosimetry systems for routine cancer patient dose verification. Methods： In vivo dosimetry, using diodes and thermoluminescent dosimeters （TLD） is performed in many radiotherapy departments to verify the dose delivered during treatment. A total of 40 TLD divided into two batches （one of 20 and other of 20 TLD） were used. Different doses of Co6~ beam were delivered to the TLD chips at different depths. Diodes were irradiated at different depths in a （30 x 30 x 30） cm3 water slab phantom with various conditions of Field sizes, monitor units and SSDs. Results： The limitation of the in-vivo dosimetry technique is that dose can only be in system readout difficulty and type of readout （TLD system and diode） as the patient dose is directly measured. Several authors have investigated the measurements was 1.3%, with a standard deviation of 2.6%. Results were normally distributed around a mean as -0.39 and 0.34 respectively. After the evaluation of in vivo dosimetry brain case as an example, the mean doses for both eyes were 1.8%, with a standard deviation of 2.7%. These results are similar to studies conducted with diodes and TLD＇s. Conclusion： The diode is superior to TLD, since the diode measurements can be obtained on line and allows an immediate check. Other advantages of diodes include high sensitivity, good spatial resolution, and small size, simplicity of used.展开更多
The research on discovery and development of new treatments for cutaneous leishmaniasis has been declared as priority. Using bioinformatics approaches, this study aimed to identify antileishmanial activity in drugs th...The research on discovery and development of new treatments for cutaneous leishmaniasis has been declared as priority. Using bioinformatics approaches, this study aimed to identify antileishmanial activity in drugs that are currently used as anti-inflammatory and wound healing by such anti-Leishmania activity was validated by in vitro and in vivo assays. In silico analysis identified 153 compounds from which 87 were selected by data mining of DrugBank database, 22 and 44 were detected by PASS （http：//pass.cribi.unipd.it） and BLAST （http：//blast.ncbi.nlm.nih. gov/） alignment, respectively. The majority of identified drugs are used as skin protector, anti-acne, anti-ulcerative （wound healer） or anti-inflammatory and few of them had specific antileishmanial activity. The efficacy as antileishmanial was validated in vitro in 12/23 tested compounds and in all seven compounds that were evaluated in in vivo assays. Notably, this is the first report of antileishmanial activity for adapalene. In conclusion, bioinformatics tools not only can help to reduce time and cost of the drug discovery process but also may increase the chance that candidates identified in silico which have a validated antileishmanial activity by combining different biological properties.展开更多
基金Supported by Major Projects of Cultivating New Varieties by Trans-genic Technology (2008ZX08012-001)~~
文摘[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.
基金Supported by grants from the Al-Hosain Hospital, Faculty of Medicine, Al Azhar University and Tanta University
文摘Objective： This study aimed to evaluate of the accuracy and efficiency of the in-vivo dosimetry systems for routine cancer patient dose verification. Methods： In vivo dosimetry, using diodes and thermoluminescent dosimeters （TLD） is performed in many radiotherapy departments to verify the dose delivered during treatment. A total of 40 TLD divided into two batches （one of 20 and other of 20 TLD） were used. Different doses of Co6~ beam were delivered to the TLD chips at different depths. Diodes were irradiated at different depths in a （30 x 30 x 30） cm3 water slab phantom with various conditions of Field sizes, monitor units and SSDs. Results： The limitation of the in-vivo dosimetry technique is that dose can only be in system readout difficulty and type of readout （TLD system and diode） as the patient dose is directly measured. Several authors have investigated the measurements was 1.3%, with a standard deviation of 2.6%. Results were normally distributed around a mean as -0.39 and 0.34 respectively. After the evaluation of in vivo dosimetry brain case as an example, the mean doses for both eyes were 1.8%, with a standard deviation of 2.7%. These results are similar to studies conducted with diodes and TLD＇s. Conclusion： The diode is superior to TLD, since the diode measurements can be obtained on line and allows an immediate check. Other advantages of diodes include high sensitivity, good spatial resolution, and small size, simplicity of used.
文摘The research on discovery and development of new treatments for cutaneous leishmaniasis has been declared as priority. Using bioinformatics approaches, this study aimed to identify antileishmanial activity in drugs that are currently used as anti-inflammatory and wound healing by such anti-Leishmania activity was validated by in vitro and in vivo assays. In silico analysis identified 153 compounds from which 87 were selected by data mining of DrugBank database, 22 and 44 were detected by PASS （http：//pass.cribi.unipd.it） and BLAST （http：//blast.ncbi.nlm.nih. gov/） alignment, respectively. The majority of identified drugs are used as skin protector, anti-acne, anti-ulcerative （wound healer） or anti-inflammatory and few of them had specific antileishmanial activity. The efficacy as antileishmanial was validated in vitro in 12/23 tested compounds and in all seven compounds that were evaluated in in vivo assays. Notably, this is the first report of antileishmanial activity for adapalene. In conclusion, bioinformatics tools not only can help to reduce time and cost of the drug discovery process but also may increase the chance that candidates identified in silico which have a validated antileishmanial activity by combining different biological properties.
