Objective： To investigate the application of percutaneous endoscopic gastrostomy （PEG） to patients with severe craniocerebral injury for the purpose of nutritional support therapy and pulmonary infection prevention...Objective： To investigate the application of percutaneous endoscopic gastrostomy （PEG） to patients with severe craniocerebral injury for the purpose of nutritional support therapy and pulmonary infection prevention. Methods： A total of 43 patients with severe craniocerebral injury admitted to our department from January 2008 to December 2012 received PEG followed by nutritional therapy. There were other 82 patients who were prescribed nasal-feeding nutrition. Nutrition status was evaluated by comparing serum albumin levels, and the incidence of pulmonary infection 1 week before and 2 weeks after operation was identified and compared. Results： Both PEG and nasal-feeding nutrition therapies have significantly elevated serum albumin levels （P〈0.05）. Serum albumin levels before and after nutritional therapies showed no significant difference between the two groups （P〉0.05）. The incidence of pulmonary infection in PEG group was significantly decreased compared with that in nasal-feeding nutrition group （P〈0.05）. Conclusion： PEG is an effective method for severe craniocerebral injury patients. It can not only provide enteral nutrition but also prevent pulmonary infection induced by esophageal reflux.展开更多
Bovine foamy virus(BFV) is a complex retrovirus that infects cattle. Like all retroviruses, BFV encodes a transactivator Tas protein(BTas) that increases gene transcription from viral promoters.BFV encodes two promote...Bovine foamy virus(BFV) is a complex retrovirus that infects cattle. Like all retroviruses, BFV encodes a transactivator Tas protein(BTas) that increases gene transcription from viral promoters.BFV encodes two promoters that can interact with BTas, a conserved promoter in the 5' long terminal repeat(LTR) and a unique internal promoter(IP). Our previous study showed that BTas is acetylated by p300 at residues K66, K109, and K110, which markedly enhanced the ability of BTas to bind to DNA. However, whether these residues are important for BFV replication was not determined. Therefore, in this study we provide direct evidence that BTas is required for BFV replication and demonstrate that residues K66, K109, and K110 are critical for BTas function and BFV replication. Full-length infectious clones were generated, which were BTas deficient or contained lysine to arginine(K→R) mutations at position 66, 109, and/or 110. In vivo data indicated that K→R mutations at positions 66, 109, and 110 in BTas impaired transactivation of both the LTR and IP promoters. In addition, the K→R mutations in full-length infectious clones reduced expression of viral proteins, and the triple mutant and BTas deletion completely abrogated viral replication. Taken together, these results indicate that lysine residues at positions 66, 109, and 110 in the BTas protein are crucial for BFV replication and suggest a potential role for BTas acetylation in regulating the viral life cycle.展开更多
文摘Objective： To investigate the application of percutaneous endoscopic gastrostomy （PEG） to patients with severe craniocerebral injury for the purpose of nutritional support therapy and pulmonary infection prevention. Methods： A total of 43 patients with severe craniocerebral injury admitted to our department from January 2008 to December 2012 received PEG followed by nutritional therapy. There were other 82 patients who were prescribed nasal-feeding nutrition. Nutrition status was evaluated by comparing serum albumin levels, and the incidence of pulmonary infection 1 week before and 2 weeks after operation was identified and compared. Results： Both PEG and nasal-feeding nutrition therapies have significantly elevated serum albumin levels （P〈0.05）. Serum albumin levels before and after nutritional therapies showed no significant difference between the two groups （P〉0.05）. The incidence of pulmonary infection in PEG group was significantly decreased compared with that in nasal-feeding nutrition group （P〈0.05）. Conclusion： PEG is an effective method for severe craniocerebral injury patients. It can not only provide enteral nutrition but also prevent pulmonary infection induced by esophageal reflux.
基金supported by grants from the National Natural Science Foundation of China(Na.31370182)Tianjin Research Program and Application Foundation and Advanced Technology(Na.12YFQNJC02300)111 Project(B08011)
文摘Bovine foamy virus(BFV) is a complex retrovirus that infects cattle. Like all retroviruses, BFV encodes a transactivator Tas protein(BTas) that increases gene transcription from viral promoters.BFV encodes two promoters that can interact with BTas, a conserved promoter in the 5' long terminal repeat(LTR) and a unique internal promoter(IP). Our previous study showed that BTas is acetylated by p300 at residues K66, K109, and K110, which markedly enhanced the ability of BTas to bind to DNA. However, whether these residues are important for BFV replication was not determined. Therefore, in this study we provide direct evidence that BTas is required for BFV replication and demonstrate that residues K66, K109, and K110 are critical for BTas function and BFV replication. Full-length infectious clones were generated, which were BTas deficient or contained lysine to arginine(K→R) mutations at position 66, 109, and/or 110. In vivo data indicated that K→R mutations at positions 66, 109, and 110 in BTas impaired transactivation of both the LTR and IP promoters. In addition, the K→R mutations in full-length infectious clones reduced expression of viral proteins, and the triple mutant and BTas deletion completely abrogated viral replication. Taken together, these results indicate that lysine residues at positions 66, 109, and 110 in the BTas protein are crucial for BFV replication and suggest a potential role for BTas acetylation in regulating the viral life cycle.
