lsochrysis zhangfiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under ni- trogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-ti...lsochrysis zhangfiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under ni- trogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-time polymerase chain reaction （qPCR） provides the possibility to investigate the gene expression levels, and a valid reference for data normalization is an essential prerequisite for firing up the analysis. In this study, five housekeeping genes, actin （ACT）, α-tubulin （TUA）, β-tubulin （TUB）, ubiquitin （UBI）, 18S rRNA （18S） and one target gene, diacylglycerol acyltransferase （DGAT）, were used for determining the reference. By analyzing the stabilities based on calculation of the stability index and on operating the two types of software, geNorm and bestkeeper, it showed that the reference genes widely used in higher plant and microalgae, such as UBI, TUA and 18S, were not the most stable ones in nitrogen-stressed 1. zhangjiangensis, and thus are not suitable for exploring the mRNA expression levels under these experi- mental conditions. Our results show that ACT together with TUB is the most feasible internal control for investigating gene expres- sion under nitrogen-stressed conditions. Our findings will contribute not only to future qPCR studies of/. zhangfiangensis, but also to verification of comparative transcriptomics studies of the microalgae under similar conditions.展开更多
Infrastructure development in Nigeria has attracted the attention of researchers in view of the attitudes of contractors and government lead-lag role playing. These attitudes led to the enactment of Fiscal Responsibil...Infrastructure development in Nigeria has attracted the attention of researchers in view of the attitudes of contractors and government lead-lag role playing. These attitudes led to the enactment of Fiscal Responsibility Act 2007 and Public Procurement Act 2007 to reduce internal control system deficiencies and budget indiscipline in the Nigerian public sector fiscal matters and procurement process. Ironically, despite these efforts, infrastructure development is still a problem affecting the lives of Nigerians as their poverty level is on the increase. This paper tests the effects of internal control system deficiency on contractors＇ capability in infrastructure development in the Nigerian public sector. Data drawn from a sample of 228 infrastructures were used for analysis. Two research questions on contractors＇ fiduciary obligation to government and capacity were analysed. Kendall＇s Tau-br correlation of coefficient and Chi-square X^2 statistics were employed for data analysis. Findings show that deficient internal control system strongly and directly breeds deficiency in the contractors＇ capability for infrastructure development. The paper recommends that oath of bribery should complement oath of secrecy to ensure that contractors and government agencies that are responsible for execution of infrastructure play to the rules of the ＂games＂, especially the provisions of Public Procurement Act 2007 and Fiscal Responsibility Act 2007. Public-private-partnership-initiative, such as build-operate and transfer method of contracting, should be encouraged to improve on contractors＇ capability and stewardship.展开更多
基金supported by the Hundred Talent Program of the Chinese Academy of Sciences (No.A1097)National High Technology Research and Development Program ‘863’ (2012AA052101)+2 种基金Liaoning Provincial Natural Science Foundation (2012010263)Knowledge Innovation Programs of Dalian Institute of Chemical Physics,CAS (K2010A13)China Postdoctoral Science Foundation Funded Project (2014M551139)
文摘lsochrysis zhangfiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under ni- trogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-time polymerase chain reaction （qPCR） provides the possibility to investigate the gene expression levels, and a valid reference for data normalization is an essential prerequisite for firing up the analysis. In this study, five housekeeping genes, actin （ACT）, α-tubulin （TUA）, β-tubulin （TUB）, ubiquitin （UBI）, 18S rRNA （18S） and one target gene, diacylglycerol acyltransferase （DGAT）, were used for determining the reference. By analyzing the stabilities based on calculation of the stability index and on operating the two types of software, geNorm and bestkeeper, it showed that the reference genes widely used in higher plant and microalgae, such as UBI, TUA and 18S, were not the most stable ones in nitrogen-stressed 1. zhangjiangensis, and thus are not suitable for exploring the mRNA expression levels under these experi- mental conditions. Our results show that ACT together with TUB is the most feasible internal control for investigating gene expres- sion under nitrogen-stressed conditions. Our findings will contribute not only to future qPCR studies of/. zhangfiangensis, but also to verification of comparative transcriptomics studies of the microalgae under similar conditions.
文摘Infrastructure development in Nigeria has attracted the attention of researchers in view of the attitudes of contractors and government lead-lag role playing. These attitudes led to the enactment of Fiscal Responsibility Act 2007 and Public Procurement Act 2007 to reduce internal control system deficiencies and budget indiscipline in the Nigerian public sector fiscal matters and procurement process. Ironically, despite these efforts, infrastructure development is still a problem affecting the lives of Nigerians as their poverty level is on the increase. This paper tests the effects of internal control system deficiency on contractors＇ capability in infrastructure development in the Nigerian public sector. Data drawn from a sample of 228 infrastructures were used for analysis. Two research questions on contractors＇ fiduciary obligation to government and capacity were analysed. Kendall＇s Tau-br correlation of coefficient and Chi-square X^2 statistics were employed for data analysis. Findings show that deficient internal control system strongly and directly breeds deficiency in the contractors＇ capability for infrastructure development. The paper recommends that oath of bribery should complement oath of secrecy to ensure that contractors and government agencies that are responsible for execution of infrastructure play to the rules of the ＂games＂, especially the provisions of Public Procurement Act 2007 and Fiscal Responsibility Act 2007. Public-private-partnership-initiative, such as build-operate and transfer method of contracting, should be encouraged to improve on contractors＇ capability and stewardship.
