China face on the serious situation about the population aging. The e-employment of elderly has become one of the approach to positively response to population aging. This paper attempts to put the elderly into the wh...China face on the serious situation about the population aging. The e-employment of elderly has become one of the approach to positively response to population aging. This paper attempts to put the elderly into the whole social system under the standpoint ofholism, then, analyze the reason of re-employment of elderly for integration and Macro from different perspective, thus, look for the significance of re-employment of elderly. Based on this, the paper put forward the suggestion for the development of the re-employment of elderly.展开更多
AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by ...AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreE RT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transformed into SW102 E. coli to remove the kanamycin unit.RESULTS A complete BAC was successfully transformed into SW102 E. coli by electroporation because BAC purified from SW102 E. coli showed the same pattern as the original BAC with Bam H I digestion. The GFPCre ERT2 fragment was deemed to have been prepared successfully because we obtained the same size fragment as expected. Homologous recombination was induced, and GFPCre ERT2 was deemed to have been inserted into the correct site of the BAC because we found the band change was the same as the expected pattern after restriction enzyme digestion. The kanamycin unit was deemed to have been removed successfully because we obtained different sizes of bands that were consistent with the results expected by PCR with different primers. CONCLUSION The construct of Gpm6 a^(GFPCreERT2) or Reelin^(GFPCreERT2) was prepared successfully, which will establish a foundation for tracing the hepatic stellate cell lineage and studying its function.展开更多
The adoption of grammatical modifier for im- plants or other kinds of biomaterials eventually absorbed by the body has been a long-standing confusing issue, and there are diverse terms in the large fields of research,...The adoption of grammatical modifier for im- plants or other kinds of biomaterials eventually absorbed by the body has been a long-standing confusing issue, and there are diverse terms in the large fields of research, which not only causes the difficulties when searching on the Internet, but also blurs the meaning and boundaries for researchers. Prior unification attempts at laws/standards set the basis for such research fields towards researching, labeling, marketing and instructions for use. Considering this, the typical gram- matical modifiers "biodegradable", "resorbable" "absorbable", along with their noun forms used in the decades of scien- tific research have been reviewed and explained, interdiscipli- nary in chemistry, ecology, materials science, biology, micro- biology, medicine, and based on usage customs, laws, stan- dards and markets. The term "biodegradable" has been not only used in biomaterials but also in ecology waste manage- ment, biomedicine and even natural environment. Mean- while, the term "resorbable" has long been used in biological reaction (osteoclast driven bone resorption), but is inappro- priate for implants that do not carry the potential to grow back into their original form. The term "absorbable" focuses more on the host metabolism to the foreign biodegradation products of the implanted material/device compared with the term "degradable/biodegradable". Meanwhile the coherence and normalization of the term "absorbable" carried by its own in laws and standards contributes as well. In general, the au- thors consider the term "absorbable" to be the best grammat- ical modifier with respect to other adjectives which share the same inherence. A further internationally unified usage is proposed by us.展开更多
文摘China face on the serious situation about the population aging. The e-employment of elderly has become one of the approach to positively response to population aging. This paper attempts to put the elderly into the whole social system under the standpoint ofholism, then, analyze the reason of re-employment of elderly for integration and Macro from different perspective, thus, look for the significance of re-employment of elderly. Based on this, the paper put forward the suggestion for the development of the re-employment of elderly.
基金Supported by National Natural Science Foundation of China,No.81300349 and No.81270532the Beijing Natural Science Foundation,No.7144216+3 种基金the Beijing Nova Program,No.Z131107000413016the Project of Science and Technology Activities of Preferred Overseas Personnel of Beijing(2014)the Project of Cultivation of High Level Medical Technical Personnel in the Health System of Beijing,No.2014-3-090 and No.2013-3-071Beijing Municipal Institute of public medical research development and reform pilot project,No.2016-2
文摘AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreE RT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transformed into SW102 E. coli to remove the kanamycin unit.RESULTS A complete BAC was successfully transformed into SW102 E. coli by electroporation because BAC purified from SW102 E. coli showed the same pattern as the original BAC with Bam H I digestion. The GFPCre ERT2 fragment was deemed to have been prepared successfully because we obtained the same size fragment as expected. Homologous recombination was induced, and GFPCre ERT2 was deemed to have been inserted into the correct site of the BAC because we found the band change was the same as the expected pattern after restriction enzyme digestion. The kanamycin unit was deemed to have been removed successfully because we obtained different sizes of bands that were consistent with the results expected by PCR with different primers. CONCLUSION The construct of Gpm6 a^(GFPCreERT2) or Reelin^(GFPCreERT2) was prepared successfully, which will establish a foundation for tracing the hepatic stellate cell lineage and studying its function.
基金supported by the National Key Research and Development Program of China (2016YFC1102402)National Natural Science Foundation of China (NSFC, 51431002)the NSFC and the Research Grants Council (RGC) of Hong Kong Joint Research Scheme (51361165101 and 5161101031)
文摘The adoption of grammatical modifier for im- plants or other kinds of biomaterials eventually absorbed by the body has been a long-standing confusing issue, and there are diverse terms in the large fields of research, which not only causes the difficulties when searching on the Internet, but also blurs the meaning and boundaries for researchers. Prior unification attempts at laws/standards set the basis for such research fields towards researching, labeling, marketing and instructions for use. Considering this, the typical gram- matical modifiers "biodegradable", "resorbable" "absorbable", along with their noun forms used in the decades of scien- tific research have been reviewed and explained, interdiscipli- nary in chemistry, ecology, materials science, biology, micro- biology, medicine, and based on usage customs, laws, stan- dards and markets. The term "biodegradable" has been not only used in biomaterials but also in ecology waste manage- ment, biomedicine and even natural environment. Mean- while, the term "resorbable" has long been used in biological reaction (osteoclast driven bone resorption), but is inappro- priate for implants that do not carry the potential to grow back into their original form. The term "absorbable" focuses more on the host metabolism to the foreign biodegradation products of the implanted material/device compared with the term "degradable/biodegradable". Meanwhile the coherence and normalization of the term "absorbable" carried by its own in laws and standards contributes as well. In general, the au- thors consider the term "absorbable" to be the best grammat- ical modifier with respect to other adjectives which share the same inherence. A further internationally unified usage is proposed by us.