In vitro regeneration systems of Atrichum mosses, Atrichurn undulatum (Hedw.) P. Beauv. and A. undulatum var. minus (Hedw.) Par. were established. After one month, soft, friable and green calli were induced successful...In vitro regeneration systems of Atrichum mosses, Atrichurn undulatum (Hedw.) P. Beauv. and A. undulatum var. minus (Hedw.) Par. were established. After one month, soft, friable and green calli were induced successfully from inoculated protonema of Atrichum mosses on MS medium containing glucose (4%) and 6-BA (0.2-2.0 mg/L). The suitable culture medium for the callus induction and regular subculture was MS medium with 1.0-2.0 mg/L 6-BA and 4% glucose. The calli of Atrichum mosses developed into protonema, when it was transferred to phytohormone-free MS medium with 4% glucose. Meanwhile, the calli developed into erect gametophytes through protonema phase on carbohydrate-free Benecke medium.展开更多
[Objective] This study was conducted to evaluate the efficiency of somatic embryogenesis and plant regeneration for an upland cotton cultivar Xinluzao33 under the induction of different hormone combinations and thus t...[Objective] This study was conducted to evaluate the efficiency of somatic embryogenesis and plant regeneration for an upland cotton cultivar Xinluzao33 under the induction of different hormone combinations and thus to determine the optimal hormone combination. [Method] Calli of Xinluzao33(Gossypium hirsutum L.) were induced from seedling hypocotyl tissue by a range of DK and BK combinations. Embryogenic calli and embryos were induced on callus-inducing medium(CIM) without any hormones. Callus appearance and quality were compared to determine which medium was the optimal for callus induction. Embryogenesis ratio was calculated to determine which medium was the best for somatic embryogenesis and plant regeneration. [Result] Callus induction rate was 100% in all the 12 hormone combinations.The calli were yellow or kelly, and their texture was loose or soft under low concentrations of DK combinations, green or white, variably compact under high concentrations of DK combinations. The calli induced by BK combinations were kelly or green, covering creamy white substance. The best medium for callus induction was DK6(0.05 mg/L 2, 4-D and 0.10 mg/L KT). Embryogenic calli were successfully induced from all the combinations. The efficiency of embryogenic callus induction,embryogenesis, and plantlet regeneration were significantly different among the 12 combinations. The result showed that the embryogenesis ratio was the highest in BK3 combination(0.50 mg/L IBA and 0.50 mg/L KT), 72.86% of embryogenic calli differentiated into somatic embryos after being cultured on CIM for 80 d, and80.93% of the somatic embryos finally regenerated into plants on SEM(somatic embryo induction medium). [Conclusion] These results indicate that hormone combination BK3(0.50 mg/LIBA and 0.50 mg/L KT) was the best medium for somatic embryogenesis and plant regeneration from Xinluzao33.展开更多
文摘In vitro regeneration systems of Atrichum mosses, Atrichurn undulatum (Hedw.) P. Beauv. and A. undulatum var. minus (Hedw.) Par. were established. After one month, soft, friable and green calli were induced successfully from inoculated protonema of Atrichum mosses on MS medium containing glucose (4%) and 6-BA (0.2-2.0 mg/L). The suitable culture medium for the callus induction and regular subculture was MS medium with 1.0-2.0 mg/L 6-BA and 4% glucose. The calli of Atrichum mosses developed into protonema, when it was transferred to phytohormone-free MS medium with 4% glucose. Meanwhile, the calli developed into erect gametophytes through protonema phase on carbohydrate-free Benecke medium.
基金Supported by the National Transgenic Major Project of China(2009ZX08009-090B)the Biological Germplasm Project of Xinjiang Production&Construction Crops(2012BD046)the Technology Plan of Xinjiang Academy of Agricultural and Reclamation Sciences(81YYD201506)
文摘[Objective] This study was conducted to evaluate the efficiency of somatic embryogenesis and plant regeneration for an upland cotton cultivar Xinluzao33 under the induction of different hormone combinations and thus to determine the optimal hormone combination. [Method] Calli of Xinluzao33(Gossypium hirsutum L.) were induced from seedling hypocotyl tissue by a range of DK and BK combinations. Embryogenic calli and embryos were induced on callus-inducing medium(CIM) without any hormones. Callus appearance and quality were compared to determine which medium was the optimal for callus induction. Embryogenesis ratio was calculated to determine which medium was the best for somatic embryogenesis and plant regeneration. [Result] Callus induction rate was 100% in all the 12 hormone combinations.The calli were yellow or kelly, and their texture was loose or soft under low concentrations of DK combinations, green or white, variably compact under high concentrations of DK combinations. The calli induced by BK combinations were kelly or green, covering creamy white substance. The best medium for callus induction was DK6(0.05 mg/L 2, 4-D and 0.10 mg/L KT). Embryogenic calli were successfully induced from all the combinations. The efficiency of embryogenic callus induction,embryogenesis, and plantlet regeneration were significantly different among the 12 combinations. The result showed that the embryogenesis ratio was the highest in BK3 combination(0.50 mg/L IBA and 0.50 mg/L KT), 72.86% of embryogenic calli differentiated into somatic embryos after being cultured on CIM for 80 d, and80.93% of the somatic embryos finally regenerated into plants on SEM(somatic embryo induction medium). [Conclusion] These results indicate that hormone combination BK3(0.50 mg/LIBA and 0.50 mg/L KT) was the best medium for somatic embryogenesis and plant regeneration from Xinluzao33.
