断指再植组织血运观察专科护理的临床应用效果

目的本文将对进行断指再植的患者进行分组护理措施,从而探讨断指再植组织血运观察专科护理的临床应用效果,为提高断指再植患者的生活质量以及预后提供可靠临床依据。方法对照组患者仅给予常规护理措施,包括体位护理、用药护理、局部护理等。研究组患者在进行常规护理措施的基础上给予血运观察专科护理,内容包括血液循环观察、饮食护理、心理护理等。结果研究组断指再植患者发生血管危象比例为27.78%,明显低于对照组断指再植患者发生血管危象比例61.11%;研究组患者发生血管危象后抢救成功率为80.00%,明显高于对照组患者发生血管危象后抢救成功率63.64%,且P<0.05,差异具有统计学意义。结论对断指再植患者进行体位护理、用药护理、局部护理、血液循环观察、饮食护理、心理护理等血运观察专科综合性护理措施,能够有效降低患者发生血管危象概率,提高患者发生血管危象后救治成功率,值得临床推广应用。

自制再植组织血循环观察记录表在显微外科的临床应用

采用自制再植组织血循环观察记录表对再植组织血液循环进行严密观察和记录,有利于医护人员准确评估患者的病情,及时制定下一步治疗护理方案,同时明确护士的责任,保障患者的安全。此观察表简单明了,可提高护士的工作质量和效率。

组织再植(移植)血管危象监测新进展

血管危象是组织再植(移植)术后重要的并发症之一,提高组织再植(移植)术后血管危象的监测能力是显微外科护理工作面临的挑战。本文综述了血管危象的分类及发生原因、血管危象的监测指标及注意事项、血管危象监测的新技术、新仪器,以及血管危象预防研究新进展,提出了在血管危象监测方面目前存在的问题及需要努力的方向。

组织移植与再植术后局部观察记录单的应用

目的预防局部血循环危象的发生。方法根据创伤显微外科术后观察指标及术后护理要求,确定观察的重点项目及程度分类,设计观察表。结果此记录单内容真实、客观,全面反映了病人局部血循环的动态变化。结论采用组织移植与再植术后局部观察记录单可以做到及时观察,及时记录,早期发现问题,从而做出准确判断,对提高医护质量、预防局部血循环危象有着重要意义。

指固有动脉静脉化在断指再植中的应用

目的 总结一侧指固有动脉静脉化在断指及复合组织瓣再植中的应用经验.方法 2012年3月-2014年9月,对22例30指断指及14例16块复合组织瓣行再植术,术中均予一侧动脉静脉化.结果 1指于远侧指间关节处离断,行再植术,非优势侧动脉静脉化,因指体挫伤严重,术后动脉一直供血不足,第3天坏死;2块复合组织瓣再植,同样行一侧动脉静脉化,术后皮缘部分坏死,经换药后愈合.其余指体及复合组织瓣均顺利成活,伤口Ⅰ期愈合.所有病例随访6-18个月,功能恢复满意,指体无明显萎缩.结论 在断指及复合组织瓣再植中,当远端无可供吻合的静脉时,临床一般都是通过“放血”疗法来解决静脉回流问题.如应用一侧指动脉静脉化之后,则无静脉回流障碍之忧,可显著提高再植成活率.

新型遮光式保温灯的应用及效果评价

[目的]探讨普通手术反光灯和新型遮光式保温灯在四肢显微血管术后提高局部温度中的应用效果。[方法]将50例四肢显微血管术后病人随机分为两组,对照组采用普通手术反光灯提高局部温度,观察组采用新型遮光式保温灯提高局部温度,观察并比较两组病人在睡眠、舒适度、再植或移植组织血运情况。[结果]两组病人在睡眠及舒适度方面差异有统计学意义(P<0.05或P<0.01),且观察组局部保温不受开窗通风的影响;再植或移植组织成活率差异无统计学意义(P>0.05)。[结论]四肢显微血管术后病人使用新型遮光式保温灯保温效果明显优于普通手术反光灯。

远端断指再植及小块游离组织再植研究

分析远端断指再植及小块游离组织再植。方法 选取2021年3月至2023年3月,我院收治的远端断指离断患者60例作为研究对象,根据不同治疗方式随机分为研究组和对照组,各30例。对照组采用常规远端断指再植技术治疗,研究组给予远端断指再植及组织再植治疗。比较两组患者治疗后的效果。结果 研究组断指再植成活率、指间关节活动度、指甲长度、两点辨别觉以及心理功能、社会功能、活力、生理职能等生活质量评分、术后3个月对患指外形满意度高于对照组,研究组术后恢复时间较对照组更短,治疗费用低于对照组,有统计学意义(P<0.05)。结论 远端断指再植及小块游离组织再植治疗可提高再植成活率,有利于患指恢复,提高患者的生活质量,增加患指外形美观度,具有应用价值。

Establishment of Rapid Propagation Technique for Cotyledon of Solanum torvum

[Objective] To research the mass propagation system for cotyledon of Solanum torvum. [Methods] With cotyledon of S. torvum as the research object, ef- fects of hormone combination on callus induction and adventitious buds differentia- tion of S. torvum were researched. [Results] With cotyledon of S. torvum as the ex- plants, the optimal culture medium for callus induction and adventitious buds differ- entiation was MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA. The induction rates of callus and adventitious bud reached 100% and 85%, respectively. The number of average buds was 6. The optimal culture medium for the induction of adventitious roots was MS＋ 0.3 mg/L IAA. The rooting rate reached 100% and the number of average roots was 9. [Conclusions] One-step induction of callus and adventitious buds simplified the mass propagation system, and enhanced the test test efficiency.s

Callus Induction and Plant Regeneration from Mature Seeds of Perennial Ryegrass

Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.

Callus induction from leaves of different paulownia species and its plantlet regeneration

The experiment was carried out on five different species of Paulownia for callus induction from leaves. MS medium was adopted as basic medium, and from different combinations of NAA and BA the suitable media were determined for callus induction, bud differentiation, and root differentiation of five different species. MS+0.5NAA+4BA, MS+0.3NAA+2BA, MS+0.5NAA+4BA, MS+0.3NAA+6BA, and MS+0.3NAA+8BA were suitable media of callus inductions of leaves, respectively, for Paulownia tomentosa, Paulownia australis, Paulownia fortunei, Paulownia elongata and P. tmentosa x P. fortunei, and MS+0.3NAA+12BA, MS+0.3NAA+12BA, MS+0.5NAA+12BA, MS+0.5NAA+12BA, and MS+0.7NAA+12BA were suitable media for bud differentiation from leaf callus respectively for above five species. The rooting media was determined as 2MS+0.1NAA, 1/2MS+0.1NAA, 1/2MS, 1/2MS+0.3NAA, and 1/2MS+0.5NAA. These results provide reference data for breeding new fine va-rieties with different kinds of Paulownia protoplasts fusions.

小组织块再植术后应用分区点剌放血法的临床观察

目的探讨无可供吻合静脉的离断小组织块再植术后,采用分区点刺放血法重建静脉回流的临床疗效。方法2013年12月至2015年8月,收治小组织块离断患者20例,其中耳、鼻、眉离断5例,指尖小组织块离断15例,均给予再植手术治疗,术中均无可供吻合静脉,术后给予抗凝、抗痉挛、抗感染治疗,应用一次性末梢采血器,采用分区点刺放血法重建静脉回流。根据再植组织块吻合动脉所在位置及动脉走行,将放血区域分为A、B、C三区,放血时首选A区。术后第1天每隔30 min放血1次,第2天每1~2h放血1次,逐渐延长,共针刺放血3~5d。结果5例头面部组织块再植后全部成活;15例指尖再植指体中,1例坏死,其余全部成活。再植体外形美观,功能良好。术后随访6~12个月,手指外形美观,皮瓣感觉接近正常,功能恢复良好。结论分区点刺放血法可有效解决无可供吻合静脉小组织块再植术后的静脉回流问题,该方法简便易行、损伤小、疗效显著。

Discussion on the Regeneration Technology of Gazania rigens L.Leaves

[Objective]The research aimed to study the regeneration technology of Cazania rigens L.leaves and screen out the optimum medium formula for the regeneration of Cazania rigens L.leaves.[Method]Using Japan imported C.rigens leaves as materials,the orthogonal test was made for the callus and adventitious buds induction in MS medium with different kinds and concentrations of hormones.The optimum medium formula for the regeneration of C.rigens leaves were screened out.[Result]On the medium of MS ＋ 0.8-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,compact type and bright green calli were formed.When the leaves were inoculated on the medium of MS ＋ 0.5-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,many adventitious shoots can be induced and the induction rate reached 100%.When strong adventitious shoots with the height of 2.0-3.0 cm were transplanted into the medium of 1/2 MS ＋0.1 mg/L NAA,the rooting situations were good and the rooting rate was 100%.[Conclusion]The research provided a new way for the rapid propagation of C.rigens and laid the foundation for the genetic transformation and new varieties breeding of C.rigens.

Effects of 2,4-D and 6-BA on Callus Induction and Plantlet Regeneration from Mature Embryos of Hsien Rice

[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong＇erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.

Establishment of Plant Regeneration System from Immature Embryos of Maize(Zea mays L.) Inbred Lines

[Objective] The aim was to explore the conditions of high frequency induction of embryonic callus and plant regeneration of maize. [Method] Immature embryos of maize inbred lines were used as explants to study the effect of different 2,4-D concentrations on the induction of callus,the effect of different 6-BA concentrations on the differentiation of test-tube plantlet,as well as the effect of different IBA concentrations on the rooting of test-tube plantlet. [Result] 2,4-D showed obvious effect on the induction of inducement rate of maize,and the optimum induction medium was：N6 ＋ 2 mg/L of 2,4-D ＋ 500 mg/L of CH ＋ 500 mg/L of Pro ＋10 mg/L of AgNO3; the optimum differentiation medium was：N6 ＋ 0.5 mg/L of BA ＋ 500 mg/L of Pro; the optimum for the rooting of test-tube plantlet was 1/2 MS ＋ 0.5 mg/L of IBA. [Conclusion] The study had provided basis for the genetic transformation of maize.

Optimization of plant regeneration system in vitro culture in wheat

Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results showed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regeneration frequency than Piclorm, with an average frequency of 54% in all treatments. Optimal condition for different genotypic rice was as following: induction medium (MSS 3AA/2) with 0.5 mg L-1 2,4-D, regeneration medium (R) with 0.01 mg L-1 2,4-D and 3 mg L-1 KT. The average regeneration frequency reached 83.3% under the condition. Correlation analysis showed that root differentiation, in different level, correlated with green spot regeneration, and with the number of regenerated plants per callus. No correlation was found between green spots regenerated and the numbers of plants regenerated per callus.

Direct Adventitious Bud Induction and Plant Regeneration of Rosa hybrida Samantha

Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on direct adventitious bud induction and plant regeneration of Rosa hybrida Samantha was investigated. The results showed that after an induction period of 8 d on MS medium with 1.5 mg L-1 TDZ and 0.05 mg L-1 NAA in the dark and a subculture on MS medium with 0.5 mg L-1 BA and 0.01 mg L-1 NAA under light, the best plant regeneration was obtained and the regeneration frequencies of leaflets and petioles were 51.8 and 10% respectively. There was no significant difference in regeneration ability between leaflets at different sites of the compound leaves, longer time of induction in the dark or high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced. This regeneration system could be applied for genetic transformation of this cultivar in the future.

Progress of safflower (Carthamus tinctorius L.) regeneration through tissue culture

Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.

Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.)

Three antibiotics ampicillin, carbenicillin, and cefotaxime were evaluated for their effects on induction, growth, and differentiation of organogenic calli, as well as rooting of regenerated shoots of three loblolly pine (Pinus taeda L.) genotypes. Of the antibiotics administered, cefotaxime maximally increased the frequency of callus formation and growth rate of organogenic calli, carbenicillin maximally increased the frequency of shoot regeneration and the average number of adventitious shoots per piece of organogenic callus, ampicillin maximally decreased the rooting frequency of regenerated shoots and mean number of roots per regenerated shoot, in comparison with antibiotic-free media. Compared with the control, ampicillin minimally increased the frequency of callus formation, cefotaxime minimally increased the frequency of shoot regeneration, and carbenicillin mini-mally decreased the rooting frequency of regenerated shoots in three loblolly pine genotypes tested. All three antibiotics in-creased the frequencies of callus formation and shoot regeneration, and reduced the rooting frequency of regenerated shoots suggested that the establishment of an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integra-tion of foreign genes into loblolly pine need to select a suitable antibiotic. This investigation could be useful for optimizing genetic transformation of conifers.