OBJECTIVE： To observe the impact of total flavo- noids from Ganshanbian （Herba Hyperici Attenuati） on the expression of vascular smooth muscle mem-brane L-type calcium channel alpha1 C subunit （CaL-olC） and ATP-s...OBJECTIVE： To observe the impact of total flavo- noids from Ganshanbian （Herba Hyperici Attenuati） on the expression of vascular smooth muscle mem-brane L-type calcium channel alpha1 C subunit （CaL-olC） and ATP-sensitive K＋ channel （KATp）-Kir6.1 mRNA, and explore the mechanisms of the antiar- rhythmic effect of Ganshanbian （Herba Hyperici At- tenuatl~ total flavonoids. METHODS： The treatment vonoids from Ganshanbian group was fed total fla- （Herba Hyperici Attenua- ti） for 7 days by gavage with 100 mg. kg-~. d 1. The blank control group and model control group were given the same amount of normal saline for 7 do Ar- rhythmias were induced by performing a myocardi- al ischemia-reperfusion and electrocardiogram was observed. Reverse transcription-polymerase chain reaction was used to detect the expression of CaL-a 1Cand KATp-Kir6.1 mRNA in the myocardial cell mem- brane of all groups of rats. RESULTS： Total flavonoids from Ganshanbian （Her- ba Hyperici Attenuat can delay the appearance of myocardial ischemia reperfusion arrhythmias, shorten the duration of myocardial ischemia reper- fusion arrhythmias, reduce heart rate, reduce cell membrane expression of CaL-a1C mRNA and en- hance the expression of K_ATp-Kir6.1 mRNA in myocar- dial ischemia-reperfusion arrhythmic rats. CONCLUSION： Total flavonoids from Ganshanbian （Herba Hyperici Attenuat can alleviate arrhythmias by affecting the expression of L-type calcium chan- nels and ATP-sensitive K_ATP channels.展开更多
基金Supported by National Natural Science Foundation of China(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology.No.81173185)Key Project of Natural Science Foundation of Heilongjiang Province(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology.No.ZD201111)+1 种基金Heilongjiang Province Graduate Students Research Key Project(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology.No.YJSCX2012-334HLJ)Outstanding Talents Support Program of Heilongjiang University of Chinese Medicine(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology,2012)
文摘OBJECTIVE： To observe the impact of total flavo- noids from Ganshanbian （Herba Hyperici Attenuati） on the expression of vascular smooth muscle mem-brane L-type calcium channel alpha1 C subunit （CaL-olC） and ATP-sensitive K＋ channel （KATp）-Kir6.1 mRNA, and explore the mechanisms of the antiar- rhythmic effect of Ganshanbian （Herba Hyperici At- tenuatl~ total flavonoids. METHODS： The treatment vonoids from Ganshanbian group was fed total fla- （Herba Hyperici Attenua- ti） for 7 days by gavage with 100 mg. kg-~. d 1. The blank control group and model control group were given the same amount of normal saline for 7 do Ar- rhythmias were induced by performing a myocardi- al ischemia-reperfusion and electrocardiogram was observed. Reverse transcription-polymerase chain reaction was used to detect the expression of CaL-a 1Cand KATp-Kir6.1 mRNA in the myocardial cell mem- brane of all groups of rats. RESULTS： Total flavonoids from Ganshanbian （Her- ba Hyperici Attenuat can delay the appearance of myocardial ischemia reperfusion arrhythmias, shorten the duration of myocardial ischemia reper- fusion arrhythmias, reduce heart rate, reduce cell membrane expression of CaL-a1C mRNA and en- hance the expression of K_ATp-Kir6.1 mRNA in myocar- dial ischemia-reperfusion arrhythmic rats. CONCLUSION： Total flavonoids from Ganshanbian （Herba Hyperici Attenuat can alleviate arrhythmias by affecting the expression of L-type calcium chan- nels and ATP-sensitive K_ATP channels.
