目的:比较药物联合预处理和单种药物预处理对沙土鼠脑缺血再灌注损伤的脑保护作用。方法:沙土鼠43只,随机分为正常对照组(A)、缺血损伤组(B)、利多卡因及异丙酚联合预处理组(C)、异丙酚预处理组(D),利多卡因预处理组(E),C、D、E预处理...目的:比较药物联合预处理和单种药物预处理对沙土鼠脑缺血再灌注损伤的脑保护作用。方法:沙土鼠43只,随机分为正常对照组(A)、缺血损伤组(B)、利多卡因及异丙酚联合预处理组(C)、异丙酚预处理组(D),利多卡因预处理组(E),C、D、E预处理组在脑缺血前24 h分别予利多卡因30 m g/kg及异丙酚100 m g/kg、异丙酚100 m g/kg、利多卡因30 m g/kg腹腔注射,对照组7只,余每组为9只,观察指标为SOD(超氧化物岐化酶)、G SH(谷光甘肽)的活性及M DA(丙二醛)、LDH(乳酸脱氢酶)、CPK(肌磷酸激酶)含量。每组随机取一左大脑皮层的1 mm×1 mm组织块作电镜,观察脑组织超微结构的改变。结果:各个药物预处理组的M DA、LDH、CPK的含量低于缺血损伤组(P<0.05,P<0.01或P<0.001),而SOD、G SH的活性高于缺血损伤组(P<0.05,P<0.01或P<0.001),而各个预处理组比较利多卡因及异丙酚联合预处理比单独预处理显示出来更好的保护作用(P<0.05或P<0.01)。与缺血再灌注组比较,各个药物预处理组在电镜超微结构均有改善,以利多卡因及异丙酚联合预处理组的改善较为明显。结论:缺血前24 h予药物预处理对沙土鼠的脑缺血再灌注损伤均有不同程度的减轻作用,并以联合预处理组的效果最为显著。展开更多
Researches in the field of the myocardial ischemia-reperfusion injury are attracting the attentions of clinicians for the treatments that protect cardiac muscle cells from being injured can not only help the patients ...Researches in the field of the myocardial ischemia-reperfusion injury are attracting the attentions of clinicians for the treatments that protect cardiac muscle cells from being injured can not only help the patients get recovery but also keep them in health. By clearing the free radicals and reducing calcium overload of myocardial cell, treatments with Danhong Injection will help myocardial cells survive from inflammatory reactions which are triggered by ischemia reperfusion so as that endothelial function will be improved and myocardial cell apoptosis will be inhibited. In all, Danhong Injection is an ideal medicine for protecting myocardial cell against ischemia reperfusion injury.展开更多
OBJECTIVE： This work aims to observe the effects of electroacupuncture on brain-derived neuro- trophic factor （BDNF） mRNA expression in mouse hippocampus following cerebral ischemia-reperfu- sion injury. METHODS： ...OBJECTIVE： This work aims to observe the effects of electroacupuncture on brain-derived neuro- trophic factor （BDNF） mRNA expression in mouse hippocampus following cerebral ischemia-reperfu- sion injury. METHODS： The models of mouse cerebral isch- emia-reperfusion injury were established. A total of 96 healthy mice were randomly assigned into 4 groups, namely, the sham surgery, model, mod- el ＋ electroacupuncture, and mode ＋ hydergine groups. Mice in the model ＋ electroacupuncturegroup were treated through electroacupuncture at the Shenshu （BL 23）, Geshu （BL 17）, and Baihui （GV 20） acupoints. Mice in the model＋hydergine group were intragastrically administered with hy- dergine （0.77 mg/kg-1.day-l）. The levels of BDNF mRNA expressions in the hippocampus were ana- lyzed through a semi-quantitative reverse transcrip- tion-polymerase chain reaction assay on days 1 and 7 after the surgeries. RESULTS： BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group （both P〈0.01）. On days 1 and 7 of the electroacupuncture treatment, BDNF mRNA expression in the mouse hippocampus of the mod- el ＋ electroacupuncture group was significantly ele- vated compared with the model group （both P〈 0.01） or the model ＋ hydergine group （both P〈 0.01）. On days 1 and 7 of the hydergine treatment, BDNF mRNA expression in the mouse hippocam- pus of the model ＋ hydergine group tended to in- crease compared with the model group; however, statistical significance was not achieved （both P〉 0.05）. CONCLUSION： Electroacupuncture treatment en- hances endogenous BDNF expression, which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells.展开更多
Methane is the simplest hydrocarbon,consisting of one carbon atom and four hydrogen atoms.It is abundant in marsh gas,livestock rumination,and combustible ice.Little is known about the use of methane in human disease ...Methane is the simplest hydrocarbon,consisting of one carbon atom and four hydrogen atoms.It is abundant in marsh gas,livestock rumination,and combustible ice.Little is known about the use of methane in human disease treatment.Current research indicates that methane is useful for treating several diseases including ischemia and reperfusion injury,and inflammatory diseases.The mechanisms underlying the protective effects of methane appear primarily to involve anti-oxidation,anti-inflammation,and anti-apoptosis.In this review,we describe the beneficial effects of methane on different diseases,summarize possible mechanisms by which methane may act in these conditions,and discuss the purpose of methane production in hypoxic conditions.Then we propose several promising directions for the future research.展开更多
文摘目的:比较药物联合预处理和单种药物预处理对沙土鼠脑缺血再灌注损伤的脑保护作用。方法:沙土鼠43只,随机分为正常对照组(A)、缺血损伤组(B)、利多卡因及异丙酚联合预处理组(C)、异丙酚预处理组(D),利多卡因预处理组(E),C、D、E预处理组在脑缺血前24 h分别予利多卡因30 m g/kg及异丙酚100 m g/kg、异丙酚100 m g/kg、利多卡因30 m g/kg腹腔注射,对照组7只,余每组为9只,观察指标为SOD(超氧化物岐化酶)、G SH(谷光甘肽)的活性及M DA(丙二醛)、LDH(乳酸脱氢酶)、CPK(肌磷酸激酶)含量。每组随机取一左大脑皮层的1 mm×1 mm组织块作电镜,观察脑组织超微结构的改变。结果:各个药物预处理组的M DA、LDH、CPK的含量低于缺血损伤组(P<0.05,P<0.01或P<0.001),而SOD、G SH的活性高于缺血损伤组(P<0.05,P<0.01或P<0.001),而各个预处理组比较利多卡因及异丙酚联合预处理比单独预处理显示出来更好的保护作用(P<0.05或P<0.01)。与缺血再灌注组比较,各个药物预处理组在电镜超微结构均有改善,以利多卡因及异丙酚联合预处理组的改善较为明显。结论:缺血前24 h予药物预处理对沙土鼠的脑缺血再灌注损伤均有不同程度的减轻作用,并以联合预处理组的效果最为显著。
文摘Researches in the field of the myocardial ischemia-reperfusion injury are attracting the attentions of clinicians for the treatments that protect cardiac muscle cells from being injured can not only help the patients get recovery but also keep them in health. By clearing the free radicals and reducing calcium overload of myocardial cell, treatments with Danhong Injection will help myocardial cells survive from inflammatory reactions which are triggered by ischemia reperfusion so as that endothelial function will be improved and myocardial cell apoptosis will be inhibited. In all, Danhong Injection is an ideal medicine for protecting myocardial cell against ischemia reperfusion injury.
基金Supported by Department of Science & Technology of He-bei Province (No. 06276102D-31)Department of Health of Hebei Province (No. 2005156)Training Program for Backbone of Scientific Research Talents of Hebei Medical University (2007)
文摘OBJECTIVE： This work aims to observe the effects of electroacupuncture on brain-derived neuro- trophic factor （BDNF） mRNA expression in mouse hippocampus following cerebral ischemia-reperfu- sion injury. METHODS： The models of mouse cerebral isch- emia-reperfusion injury were established. A total of 96 healthy mice were randomly assigned into 4 groups, namely, the sham surgery, model, mod- el ＋ electroacupuncture, and mode ＋ hydergine groups. Mice in the model ＋ electroacupuncturegroup were treated through electroacupuncture at the Shenshu （BL 23）, Geshu （BL 17）, and Baihui （GV 20） acupoints. Mice in the model＋hydergine group were intragastrically administered with hy- dergine （0.77 mg/kg-1.day-l）. The levels of BDNF mRNA expressions in the hippocampus were ana- lyzed through a semi-quantitative reverse transcrip- tion-polymerase chain reaction assay on days 1 and 7 after the surgeries. RESULTS： BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group （both P〈0.01）. On days 1 and 7 of the electroacupuncture treatment, BDNF mRNA expression in the mouse hippocampus of the mod- el ＋ electroacupuncture group was significantly ele- vated compared with the model group （both P〈 0.01） or the model ＋ hydergine group （both P〈 0.01）. On days 1 and 7 of the hydergine treatment, BDNF mRNA expression in the mouse hippocam- pus of the model ＋ hydergine group tended to in- crease compared with the model group; however, statistical significance was not achieved （both P〉 0.05）. CONCLUSION： Electroacupuncture treatment en- hances endogenous BDNF expression, which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells.
文摘Methane is the simplest hydrocarbon,consisting of one carbon atom and four hydrogen atoms.It is abundant in marsh gas,livestock rumination,and combustible ice.Little is known about the use of methane in human disease treatment.Current research indicates that methane is useful for treating several diseases including ischemia and reperfusion injury,and inflammatory diseases.The mechanisms underlying the protective effects of methane appear primarily to involve anti-oxidation,anti-inflammation,and anti-apoptosis.In this review,we describe the beneficial effects of methane on different diseases,summarize possible mechanisms by which methane may act in these conditions,and discuss the purpose of methane production in hypoxic conditions.Then we propose several promising directions for the future research.
