Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegr...Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.展开更多
The dry-hot valley of the Jinsha River is one of the typical eco-fragile areas in Southwest China, as well as a focus of revegetation study in the upper and middle reaches of the Changjiang River. Due to its extremely...The dry-hot valley of the Jinsha River is one of the typical eco-fragile areas in Southwest China, as well as a focus of revegetation study in the upper and middle reaches of the Changjiang River. Due to its extremely dry and hot climate, severely degraded vegetation and the intense soil and water loss, there are extreme difficulties in vegetation restoration in this area and no great breakthrough has ever been achieved on studies of revegetation over the last several decades. Through over ten years’ research conducted in the typical areas-the Yuanmou dry-hot valley, the authors found that the lithologic property is one of the crucial factors determining soil moisture conditions and vegetation types in the dry-hot valley, and the rainfall infiltration capability is also one of the key factors affecting the tree growth. Then the revegetation zoning based on different slopes was conducted and revegetation patterns for different zones were proposed.展开更多
Diversity, density and species composition of naturally regenerated woody plants under Eucalyptus grandis plantation and the adjacent natural forest were investigated and compared. Twenty plots, with an area of 20 m &...Diversity, density and species composition of naturally regenerated woody plants under Eucalyptus grandis plantation and the adjacent natural forest were investigated and compared. Twenty plots, with an area of 20 m × 20 m for each, were established in both of E. grand＆ plantation and adjacent natural forest, independently. In each plot, species name, abundance, diameter and height were recorded. Numbers of seedling were collected in five sub-plots （4 m2） within each major plot. A total of 46 species in the plantation, and 52 species in the natural forest, which belongs to 36 families were recorded. The diversity of species （H＇） is 2.19 in the plantation and 2.74 in the natural forest. The density of understory woody plant was 3842 stems/ha in the plantation and 4122 stems/ha in the natural forest. The densities of seedlings in the natural forest and the plantation were 8101 stems/ha and 4151 stems/ha, respectively. High similarity of woody species composition was found between the natural forest and the plantation. The E. grand＆ plantation was found favoring the regeneration and growth of Millitiaferruginia and Coffea arabica in a much better way than other underneath woody species.展开更多
[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiati...[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong＇erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.展开更多
Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carri...Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.展开更多
For the carbon-neutral,a multi-carrier renewable energy system(MRES),driven by the wind,solar and geothermal,was considered as an effective solution to mitigate CO2emissions and reduce energy usage in the building sec...For the carbon-neutral,a multi-carrier renewable energy system(MRES),driven by the wind,solar and geothermal,was considered as an effective solution to mitigate CO2emissions and reduce energy usage in the building sector.A proper sizing method was essential for achieving the desired 100%renewable energy system of resources.This paper presented a bi-objective optimization formulation for sizing the MRES using a constrained genetic algorithm(GA)coupled with the loss of power supply probability(LPSP)method to achieve the minimal cost of the system and the reliability of the system to the load real time requirement.An optimization App has been developed in MATLAB environment to offer a user-friendly interface and output the optimized design parameters when given the load demand.A case study of a swimming pool building was used to demonstrate the process of the proposed design method.Compared to the conventional distributed energy system,the MRES is feasible with a lower annual total cost(ATC).Additionally,the ATC decreases as the power supply reliability of the renewable system decreases.There is a decrease of 24%of the annual total cost when the power supply probability is equal to 8%compared to the baseline case with 0%power supply probability.展开更多
[Objective] To screen ratooning rice varieties for the ratooning rice-rape cropping planting pattern in Ganfu Plain. [Method] The growth period, plant morphology, yield and its component of 11 varieties at the first a...[Objective] To screen ratooning rice varieties for the ratooning rice-rape cropping planting pattern in Ganfu Plain. [Method] The growth period, plant morphology, yield and its component of 11 varieties at the first and rebirth season were compared and analyzed. [Result] The ratooning rice varieties such as Heliangyou -1, Y Liangyou 6, Zhunliangyou 608 and Jingliangyouhuazhan were suitable for the production and application in Ganfu Plain. Heliangyou 1 and Zhunliangyou 608 showed the characteristics of early maturity, easy to achieve high yield and stable production. [Conclusion] It suggests that Heliangyou 1 and Zhunliangyou 608 should be selected as preferred varieties for the planting pattern of ratooning rice-rape cropping.展开更多
The species diversity at the regeneration stage, inflenced by different water levels, is important for community composition in the later growing season.Regeneration diversity of Carex lasiocarpa community under diffe...The species diversity at the regeneration stage, inflenced by different water levels, is important for community composition in the later growing season.Regeneration diversity of Carex lasiocarpa community under different water levels was studied at two stages, recruitment and adult, in the Sanjiang Plain, Heilongjiang Province, China.The results showed that, at the two growing stages, important value of C.lasiocarpa population and species richness of the community decreased with the increasing water level, while the Simpson and Shannon-Wiener diversity indexes and Pielou evenness index increased.Under different water levels, community diversities were higher at the recruitment stage, while population important values of C.lasiocarpa were higher at the adult stage.Indexes in vegetation evaluation must be chosen prudentially for successful restoration and effective management of wetlands, and especially for wetland restoration, the optimal time should be selected according to the restoration objectives and costs.展开更多
The increasing shortage of donors and the adverse effects of immunosuppression have restricted the impact of solid organ transplantation.Despite the initial promising developments in xenotransplantation,roadblocks sti...The increasing shortage of donors and the adverse effects of immunosuppression have restricted the impact of solid organ transplantation.Despite the initial promising developments in xenotransplantation,roadblocks still need to be overcome and this form of organ support remains a long way from clinical practice.While hepatocyte transplantation may be effectively correct metabolic defects,it is far less effective in restoring liver function than liver transplantation.Tissue engineering,using extracellular matrix scaffolds with an intact but decellularized vascular network that is repopulated with autologous or allogeneic stem cells and/or adult cells,holds great promise for the treatment of failure of organs within gastrointestinal tract,such as endstage liver disease,pancreatic insufficiency,bowel failure and type 1 diabetes.Particularly in the liver field,where there is a significant mortality of patients awaiting transplant,human bioengineering may offer a source of readily available organs for transplantation.The use of autologous cells will mitigate the need for long term immunosuppression thus removing a major hurdle in transplantation.展开更多
Detailed studies on population structure and regeneration ecology ofllex khasiana were carried out at five representative natural populations i.e. Mylliem （Population - I）, Upper Shillong （Population - II）, Shillo...Detailed studies on population structure and regeneration ecology ofllex khasiana were carried out at five representative natural populations i.e. Mylliem （Population - I）, Upper Shillong （Population - II）, Shillong Peak （Population -III）, Laitkor （Population - IV） and Nongpiyur （Population - V） to probe into the responsible affecting factors for its population structure and regeneration ability in these populations. Experimental observation under three controlled light conditions （full sunlight （100%）, intermediate sunlight （50%） and low sunlight （30%）） showed that the growth status and survival rates of seedlings under intermediate and low light were better than those under the high light condition. The density-diameter distribution in population of adult trees （〉Scm dbh） exhibited a typical character of a regenerating population. A higher seedling mortality rate in natural populations of I. khasiana was most probably due to increase in light intensity following vegetation destruction and other anthropogenic disturbances that cause opening of forest canopy. The findings of the present study would be of immense value in formulating appropriate conservation measures for the species.展开更多
基金Supported by National Natural Science Foundation of China(30471274)~~
文摘Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.
基金Under the auspices of the National Natural Science Foundation of China (No .30470297)and theNationalBasicRe-searchProgram ofChina (973 Program)(No .2003CB415201 )
文摘The dry-hot valley of the Jinsha River is one of the typical eco-fragile areas in Southwest China, as well as a focus of revegetation study in the upper and middle reaches of the Changjiang River. Due to its extremely dry and hot climate, severely degraded vegetation and the intense soil and water loss, there are extreme difficulties in vegetation restoration in this area and no great breakthrough has ever been achieved on studies of revegetation over the last several decades. Through over ten years’ research conducted in the typical areas-the Yuanmou dry-hot valley, the authors found that the lithologic property is one of the crucial factors determining soil moisture conditions and vegetation types in the dry-hot valley, and the rainfall infiltration capability is also one of the key factors affecting the tree growth. Then the revegetation zoning based on different slopes was conducted and revegetation patterns for different zones were proposed.
基金provided by the Canadian International Development Agency (CIDA)
文摘Diversity, density and species composition of naturally regenerated woody plants under Eucalyptus grandis plantation and the adjacent natural forest were investigated and compared. Twenty plots, with an area of 20 m × 20 m for each, were established in both of E. grand＆ plantation and adjacent natural forest, independently. In each plot, species name, abundance, diameter and height were recorded. Numbers of seedling were collected in five sub-plots （4 m2） within each major plot. A total of 46 species in the plantation, and 52 species in the natural forest, which belongs to 36 families were recorded. The diversity of species （H＇） is 2.19 in the plantation and 2.74 in the natural forest. The density of understory woody plant was 3842 stems/ha in the plantation and 4122 stems/ha in the natural forest. The densities of seedlings in the natural forest and the plantation were 8101 stems/ha and 4151 stems/ha, respectively. High similarity of woody species composition was found between the natural forest and the plantation. The E. grand＆ plantation was found favoring the regeneration and growth of Millitiaferruginia and Coffea arabica in a much better way than other underneath woody species.
基金Supported by the National Natural Science Foundation (30571049)~~
文摘[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong＇erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.
基金Supported by the National Natural Science Foundation of China Granted Project(81173484)
文摘Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.
基金Project(52108101)supported by the National Natural Science Foundation of ChinaProjects(2020GK4057,2021JJ40759)supported by the Hunan Provincial Science and Technology Department,China。
文摘For the carbon-neutral,a multi-carrier renewable energy system(MRES),driven by the wind,solar and geothermal,was considered as an effective solution to mitigate CO2emissions and reduce energy usage in the building sector.A proper sizing method was essential for achieving the desired 100%renewable energy system of resources.This paper presented a bi-objective optimization formulation for sizing the MRES using a constrained genetic algorithm(GA)coupled with the loss of power supply probability(LPSP)method to achieve the minimal cost of the system and the reliability of the system to the load real time requirement.An optimization App has been developed in MATLAB environment to offer a user-friendly interface and output the optimized design parameters when given the load demand.A case study of a swimming pool building was used to demonstrate the process of the proposed design method.Compared to the conventional distributed energy system,the MRES is feasible with a lower annual total cost(ATC).Additionally,the ATC decreases as the power supply reliability of the renewable system decreases.There is a decrease of 24%of the annual total cost when the power supply probability is equal to 8%compared to the baseline case with 0%power supply probability.
文摘[Objective] To screen ratooning rice varieties for the ratooning rice-rape cropping planting pattern in Ganfu Plain. [Method] The growth period, plant morphology, yield and its component of 11 varieties at the first and rebirth season were compared and analyzed. [Result] The ratooning rice varieties such as Heliangyou -1, Y Liangyou 6, Zhunliangyou 608 and Jingliangyouhuazhan were suitable for the production and application in Ganfu Plain. Heliangyou 1 and Zhunliangyou 608 showed the characteristics of early maturity, easy to achieve high yield and stable production. [Conclusion] It suggests that Heliangyou 1 and Zhunliangyou 608 should be selected as preferred varieties for the planting pattern of ratooning rice-rape cropping.
基金Under the auspices of the National Basic Research Program of China (No. 2009CB421103)Northeast Revitalization Program,Chinese Academy of Sciences (No. DBZX-2-024)
文摘The species diversity at the regeneration stage, inflenced by different water levels, is important for community composition in the later growing season.Regeneration diversity of Carex lasiocarpa community under different water levels was studied at two stages, recruitment and adult, in the Sanjiang Plain, Heilongjiang Province, China.The results showed that, at the two growing stages, important value of C.lasiocarpa population and species richness of the community decreased with the increasing water level, while the Simpson and Shannon-Wiener diversity indexes and Pielou evenness index increased.Under different water levels, community diversities were higher at the recruitment stage, while population important values of C.lasiocarpa were higher at the adult stage.Indexes in vegetation evaluation must be chosen prudentially for successful restoration and effective management of wetlands, and especially for wetland restoration, the optimal time should be selected according to the restoration objectives and costs.
文摘The increasing shortage of donors and the adverse effects of immunosuppression have restricted the impact of solid organ transplantation.Despite the initial promising developments in xenotransplantation,roadblocks still need to be overcome and this form of organ support remains a long way from clinical practice.While hepatocyte transplantation may be effectively correct metabolic defects,it is far less effective in restoring liver function than liver transplantation.Tissue engineering,using extracellular matrix scaffolds with an intact but decellularized vascular network that is repopulated with autologous or allogeneic stem cells and/or adult cells,holds great promise for the treatment of failure of organs within gastrointestinal tract,such as endstage liver disease,pancreatic insufficiency,bowel failure and type 1 diabetes.Particularly in the liver field,where there is a significant mortality of patients awaiting transplant,human bioengineering may offer a source of readily available organs for transplantation.The use of autologous cells will mitigate the need for long term immunosuppression thus removing a major hurdle in transplantation.
文摘Detailed studies on population structure and regeneration ecology ofllex khasiana were carried out at five representative natural populations i.e. Mylliem （Population - I）, Upper Shillong （Population - II）, Shillong Peak （Population -III）, Laitkor （Population - IV） and Nongpiyur （Population - V） to probe into the responsible affecting factors for its population structure and regeneration ability in these populations. Experimental observation under three controlled light conditions （full sunlight （100%）, intermediate sunlight （50%） and low sunlight （30%）） showed that the growth status and survival rates of seedlings under intermediate and low light were better than those under the high light condition. The density-diameter distribution in population of adult trees （〉Scm dbh） exhibited a typical character of a regenerating population. A higher seedling mortality rate in natural populations of I. khasiana was most probably due to increase in light intensity following vegetation destruction and other anthropogenic disturbances that cause opening of forest canopy. The findings of the present study would be of immense value in formulating appropriate conservation measures for the species.
