[ Objective ] The insecticidal and antibacterial bioactivity of Wikstroemia chamaedaphne Meissn were screened and bioactive substances in it were separated and purified. [ Method] The Wikstroemia chamaedaphne Meissn w...[ Objective ] The insecticidal and antibacterial bioactivity of Wikstroemia chamaedaphne Meissn were screened and bioactive substances in it were separated and purified. [ Method] The Wikstroemia chamaedaphne Meissn was conducted ultrasonic extraction in petroleum ether, ethyl acetate and methanol. The insecticidal activity of Wikstroemia chamaedaphne Meissn to Mythimna separata walker and aphid were determined. The antibacterial activity of Wikstroemia chamaedaphne Meissn to Fusarium graminearu, Glomerella cingulata, F. oxysporium f. sp niveum, Alternaria solani and Fusarium oxysporium were also determined. The bioactivity-guided methods such as opencolumn chromatography and Pre-HPLC method were used to separate active components in petroleum ether extract from Wikstroemia chamaedaphne Meissn. [ Result] When the concentration was 500 mg/L, 3 kinds of extracts from Wikstroemia chamaedaphne Meissn didn' t show obvious antibacterial bioactivity to 5 kinds of test samples. When the concentration was 5%, petroleum ether extract show certain topical toxicity to aphids. The ethyl acetate extract showed certain antifeedant activity to 3^rd instar Larvae of Mythimna separata Walker. The fraction F4 of petroleum ether extract possessed highest topical toxicity to aphids and the lethality was 60.00%. [ Conclusion] Wikstroemia chamaedaphne Meissn contained many insecticidal constituents whose active parts and mechanism were needed further researches.展开更多
[Objective] This study was to evaluate the ecological suitability of agricultural land in western Jilin Province, with the aim to provide basis for the effective application of agricultural resources and the structura...[Objective] This study was to evaluate the ecological suitability of agricultural land in western Jilin Province, with the aim to provide basis for the effective application of agricultural resources and the structural adjustment of land use. [Method] The evaluation index system was constructed based on fuzzy mathematic method according to the principles of systematic, dominant, effectiveness and feasibility; based on law of tolerance, reasonable evaluation criteria were determined according to the ecological amplitude of crops; based on GIS processing, the initial data completed the mathematical operation by using the VBA program in Excel. [Result] The area of agricultural land grade I was 5 512 km2, grade II of 25 985 km2, grade Ill of 7 907 km2, and area of land not suitable for agriculture was 6 312 km2. According to the evaluation results, the key areas for land use adjustment were Zhenlai County, Da'an County, Tongyu County and western Changling County. The directions of land use adjustment included improving irrigation and drainage conditions, governing land salinization and conversion of cropland to forest and grassland. [Conclusion] This study provided basis for the effective application of agricultural resources and ecological environment construction in western Jilin Province.展开更多
[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] Wi...[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.展开更多
Based on theories of protective forests and landscape ecology, the reasonableness of structures and patterns of shelterbelt system at Beizang Town, Daxing County, Beijing were analyzed and assessed from the two scales...Based on theories of protective forests and landscape ecology, the reasonableness of structures and patterns of shelterbelt system at Beizang Town, Daxing County, Beijing were analyzed and assessed from the two scales of forest belts and networks, by integrating uses of field investigation, GIS and RS techniques. Results showed that the existent main belt (3-12 m in width) was too narrow, while the assistant belt (3-27.1 m in width) was too wide; the species composition of the existent shelterbelts was single, and the structures and patterns of the shelterbelt system were unreasonable. It is suggested that the structure of the main and the assistant belts should be changed, such as increasing the width of main belts, decreasing the width of assistant belt, and planting more mixed species, and the pattern with arbores in the middle and shrubs in the sides of belts could be taken into account. For the landscape structure of forest network after regenerating or reconstruction, the grid number of closed network should be 13 per km2 and the minimum number of belts should be 34 per km2. This study also testified that integrating GIS and remote technique with landscape ecology could provide an effective method for reasonable reconstruction of the structures and patterns of shelterbelts system.展开更多
基金Supported by School Project of Yan’an University (YD2005-042)~~
文摘[ Objective ] The insecticidal and antibacterial bioactivity of Wikstroemia chamaedaphne Meissn were screened and bioactive substances in it were separated and purified. [ Method] The Wikstroemia chamaedaphne Meissn was conducted ultrasonic extraction in petroleum ether, ethyl acetate and methanol. The insecticidal activity of Wikstroemia chamaedaphne Meissn to Mythimna separata walker and aphid were determined. The antibacterial activity of Wikstroemia chamaedaphne Meissn to Fusarium graminearu, Glomerella cingulata, F. oxysporium f. sp niveum, Alternaria solani and Fusarium oxysporium were also determined. The bioactivity-guided methods such as opencolumn chromatography and Pre-HPLC method were used to separate active components in petroleum ether extract from Wikstroemia chamaedaphne Meissn. [ Result] When the concentration was 500 mg/L, 3 kinds of extracts from Wikstroemia chamaedaphne Meissn didn' t show obvious antibacterial bioactivity to 5 kinds of test samples. When the concentration was 5%, petroleum ether extract show certain topical toxicity to aphids. The ethyl acetate extract showed certain antifeedant activity to 3^rd instar Larvae of Mythimna separata Walker. The fraction F4 of petroleum ether extract possessed highest topical toxicity to aphids and the lethality was 60.00%. [ Conclusion] Wikstroemia chamaedaphne Meissn contained many insecticidal constituents whose active parts and mechanism were needed further researches.
基金Supported by the National Natural Science Foundation of China(41072196)~~
文摘[Objective] This study was to evaluate the ecological suitability of agricultural land in western Jilin Province, with the aim to provide basis for the effective application of agricultural resources and the structural adjustment of land use. [Method] The evaluation index system was constructed based on fuzzy mathematic method according to the principles of systematic, dominant, effectiveness and feasibility; based on law of tolerance, reasonable evaluation criteria were determined according to the ecological amplitude of crops; based on GIS processing, the initial data completed the mathematical operation by using the VBA program in Excel. [Result] The area of agricultural land grade I was 5 512 km2, grade II of 25 985 km2, grade Ill of 7 907 km2, and area of land not suitable for agriculture was 6 312 km2. According to the evaluation results, the key areas for land use adjustment were Zhenlai County, Da'an County, Tongyu County and western Changling County. The directions of land use adjustment included improving irrigation and drainage conditions, governing land salinization and conversion of cropland to forest and grassland. [Conclusion] This study provided basis for the effective application of agricultural resources and ecological environment construction in western Jilin Province.
基金Supported by the National High Technology Research and Develop-ment Program of China(No.2008AA10Z136)the Program of Liaoning Provincial Science-Technology Department(20070567)~~
文摘[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.
基金This research was funded by a sub-program of the Ninth Five Years of China: "Study and demonstration of combating technique of sandy disasters in sub-humid-semi-dry zone Yongding River Sandlot"(96-017-01-01).
文摘Based on theories of protective forests and landscape ecology, the reasonableness of structures and patterns of shelterbelt system at Beizang Town, Daxing County, Beijing were analyzed and assessed from the two scales of forest belts and networks, by integrating uses of field investigation, GIS and RS techniques. Results showed that the existent main belt (3-12 m in width) was too narrow, while the assistant belt (3-27.1 m in width) was too wide; the species composition of the existent shelterbelts was single, and the structures and patterns of the shelterbelt system were unreasonable. It is suggested that the structure of the main and the assistant belts should be changed, such as increasing the width of main belts, decreasing the width of assistant belt, and planting more mixed species, and the pattern with arbores in the middle and shrubs in the sides of belts could be taken into account. For the landscape structure of forest network after regenerating or reconstruction, the grid number of closed network should be 13 per km2 and the minimum number of belts should be 34 per km2. This study also testified that integrating GIS and remote technique with landscape ecology could provide an effective method for reasonable reconstruction of the structures and patterns of shelterbelts system.