以采自湖北省荆州市和黄冈市的农田土壤作为供试土样,分别采用土壤样品冰浴后上清液100倍稀释、冰浴后悬浊液1 000倍稀释和离心去碳源3种方法,利用多功能酶标仪间隔12h检测D值,并计算AWCD(average well color development,每孔平均颜色...以采自湖北省荆州市和黄冈市的农田土壤作为供试土样,分别采用土壤样品冰浴后上清液100倍稀释、冰浴后悬浊液1 000倍稀释和离心去碳源3种方法,利用多功能酶标仪间隔12h检测D值,并计算AWCD(average well color development,每孔平均颜色变化)值来探究分析土壤肥力高、富含有机质、可利用碳源丰富的农田土壤微生物代谢功能的BIOLOG-ECO微平板技术适宜方法。结果表明,土样冰浴后上清液100倍稀释的方法不能有效去除土壤中可利用碳源,不能准确反映土壤微生物对碳源的利用程度。而冰浴后悬浊液1 000倍稀释、离心去碳源都能准确显示土壤微生物的活性变化,但是两者AWCD的标准差有极显著的差异,比较而言,离心去碳源的方法能更稳定地检测土壤微生物群落的代谢能力。研究结果为利用BIOLOG-ECO微平板技术检测富含有机质的农田土壤微生物代谢功能多样性提供了有价值的信息。展开更多
Cropping activities may affect soil microbial activities and biomass,which would affect C and N cycling in soil and thus the crop yields and quality.In the present study,a combination of microcalorimetric,enzyme activ...Cropping activities may affect soil microbial activities and biomass,which would affect C and N cycling in soil and thus the crop yields and quality.In the present study,a combination of microcalorimetric,enzyme activity(sucrase,urease,catalase,and fluorescein diacetate hydrolysis),and real-time polymerase chain reaction(RT-PCR) analyses was used to investigate microbial status of farmland soils,collected from 5 different sites in Huazhong Agriculture University,China.Our results showed that among the 5sites,both positive and negative impacts of cropping activities on soil microbial activity were observed.Enzyme activity analysis showed that cropping activities reduced soil sucrase and urease activities,which would influence the C and N cycles in soil.Much more attentions should be given to microbial status affected by cropping activities in future.According to the correlation analysis,fluorescein diacetate hydrolysis showed a significantly(P < 0.05) negative correlation with the time to reach the maximum power output(R ——0.898),but a significantly(P < 0.05) positive correlation with bacterial gene copy number(R = 0.817).Soil catalase activity also showed a significantly(P < 0.05) positive correlation with bacterial gene copy number(R = 0.965).Using combined methods would provide virtual information of soil microbial status.展开更多
文摘以采自湖北省荆州市和黄冈市的农田土壤作为供试土样,分别采用土壤样品冰浴后上清液100倍稀释、冰浴后悬浊液1 000倍稀释和离心去碳源3种方法,利用多功能酶标仪间隔12h检测D值,并计算AWCD(average well color development,每孔平均颜色变化)值来探究分析土壤肥力高、富含有机质、可利用碳源丰富的农田土壤微生物代谢功能的BIOLOG-ECO微平板技术适宜方法。结果表明,土样冰浴后上清液100倍稀释的方法不能有效去除土壤中可利用碳源,不能准确反映土壤微生物对碳源的利用程度。而冰浴后悬浊液1 000倍稀释、离心去碳源都能准确显示土壤微生物的活性变化,但是两者AWCD的标准差有极显著的差异,比较而言,离心去碳源的方法能更稳定地检测土壤微生物群落的代谢能力。研究结果为利用BIOLOG-ECO微平板技术检测富含有机质的农田土壤微生物代谢功能多样性提供了有价值的信息。
基金supported by the Public Welfare Project of Ministry of Environmental Protection of China(Nos.201409042 and 201509049)the National Natural Science Foundation of China(Nos. 41430106,41273092,U1402234,and 41603083)
文摘Cropping activities may affect soil microbial activities and biomass,which would affect C and N cycling in soil and thus the crop yields and quality.In the present study,a combination of microcalorimetric,enzyme activity(sucrase,urease,catalase,and fluorescein diacetate hydrolysis),and real-time polymerase chain reaction(RT-PCR) analyses was used to investigate microbial status of farmland soils,collected from 5 different sites in Huazhong Agriculture University,China.Our results showed that among the 5sites,both positive and negative impacts of cropping activities on soil microbial activity were observed.Enzyme activity analysis showed that cropping activities reduced soil sucrase and urease activities,which would influence the C and N cycles in soil.Much more attentions should be given to microbial status affected by cropping activities in future.According to the correlation analysis,fluorescein diacetate hydrolysis showed a significantly(P < 0.05) negative correlation with the time to reach the maximum power output(R ——0.898),but a significantly(P < 0.05) positive correlation with bacterial gene copy number(R = 0.817).Soil catalase activity also showed a significantly(P < 0.05) positive correlation with bacterial gene copy number(R = 0.965).Using combined methods would provide virtual information of soil microbial status.
