[ Objective] The aim was to study method for extracting DNA from pteridophyta and provide basis for further study on genetic diversity and taxonomy. [ Method] By changing the dosage of reagent and operating method, CT...[ Objective] The aim was to study method for extracting DNA from pteridophyta and provide basis for further study on genetic diversity and taxonomy. [ Method] By changing the dosage of reagent and operating method, CTAB method for extracting DNA was improved. [ Result] The results showed that the improved CTAB method could extract high-quality DNA from pteridophyta. [ Conclusion] The study improved method for extracting DNA from pteridophyta.展开更多
Fenlong green ecological agriculture technology （Fenlong technology）, a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation techniqu...Fenlong green ecological agriculture technology （Fenlong technology）, a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation technique by the Ministry of Agriculture of China. It replaces the traditional plowshare with spiral drill, and its tilth depth is twice deeper than that by tractor tilthing. It also extends soil nutrient, moisture, oxygen and microorganism, the so-called ＂Four pools＂. Soil nutrient, oxygen, microorganism, light and rainfall use ratio is increased by 10%-100%, creating a platform for natural increase of more than 10% of crop yield. Its application to over 20 kinds of crops in 21 provinces has proved that the yield increases 10-30% with quality enhancing 5% and double water retaining capacity but no more input. When the application area of Fenlong could reach 67 million hm2, the amount of fertilizer can be reduced by 40-50 billion kg, saving 120-150 billion Yuan. In this paper, we put forward the strategy of ＂4＋1＂ （arable, saline-alkali soil, grasslands, Sponge City ＋ rivers） green development in China, and deepened the Fenlong cultivated tilled layer from 16.5 cm to 35 cm for 67 million hm2 arable land, ridged 13.3 million hm2 of saline-alkali soil for 35 cm, and also 35 cm for 67 million hm2 degraded steppe, which could have the following 3 effects： first, the 147 million hm2 of land with Fenlong cultivation could increase loosing soil to 315.491 billion m3, in＊ creasing by 159.26% for 120 million hm2 of arable land with the average tilled layer of 16.5 cm, which has loosing soil of only 198.1 billion m3, that is, the space of the land increases 1.6 times. Second, every hectare of plowland could store up to 450 m3/hm2 of natural rainfall, and the unused 60 m3 of saline-alkali soil and grasslands could store water of 102 billion m3, showing an increase of over 88.89% for the current plowland storage of 54 billion m3 at now, that is, double the natural rainfall storage capacity. Third, the two multiple increase of natural resources application can bring trillions of resource activation, environmental cleaning, food security, citizens, health, economic, ecological and social benefits, and makes the Chinese nation move forward in green development. Its application in ＂big scientific research＂ and ＂One Belt And One Road＂ will contribute Chinese strength to the world.展开更多
To understand the high yield and efficiency technique in hybrid rice Zhongzheyou No. 1, we conducted the correlation analysis, regression analysis and path analysis of hybrid rice Zhongzheyou No. 1 based on the data o...To understand the high yield and efficiency technique in hybrid rice Zhongzheyou No. 1, we conducted the correlation analysis, regression analysis and path analysis of hybrid rice Zhongzheyou No. 1 based on the data of its ear, grain and weight at different yield levels. From this study, we put forward the high yield and efficiency technique in Zhongzheyou No. 1 .. on the basis of certain effective ear number, filled grains per ear should be mainly targeted with a consideration to 1 000-grain weight.展开更多
[Objective] The aim of this study was to investigate the preparation method and amplification system of antagonistic streptomyces DNA templates based on AFLP assays, and also provide a basis for the application of AFL...[Objective] The aim of this study was to investigate the preparation method and amplification system of antagonistic streptomyces DNA templates based on AFLP assays, and also provide a basis for the application of AFLP technology in the analysis of streptomyces or even actinomyces. [Method] The DNAs were extracted by the modified CTAB method and amplified by the Pst Ⅰ/Mse Ⅰ AFLP kit and its reaction system. The amplified products were analyzed by the denatured polyacrylamide gel electrophoresis. [Result] The genomic DNAs of ten antagonistic strains of Streptomyces were extracted and tested. The result of 0.8% agarose gel electrophoresis showed that the major DNA bands were clear without degradation and RNA residue, with the fragment sizes ranging from 37.64 to 40.86 Kb. By ultraviolet spectrophotometry, the OD260/OD280 values varying from 1.625 to 1.833 were obtained. Furthermore, the agarose gel electrophoresis of DNA products digested by Pst Ⅰ/Mse Ⅰ presented the dispersed fluorescent long band, which indicated that the enzymatic hydrolysis was fully carried out. The amplified bands of DNA templates by the screened three pairs of primers were clear with rich polymorphism. [Conclusion] The preparation method and amplification system of DNA template established in this study can be used in the AFLP analysis of Streptomyces.展开更多
Two-oriented agriculture was a complex organism coupling production,economics,society and ecology.Its development process was affected by various factors such as producers,nature,society,etc.In order to overcome measu...Two-oriented agriculture was a complex organism coupling production,economics,society and ecology.Its development process was affected by various factors such as producers,nature,society,etc.In order to overcome measurement error of traditional data envelopment analysis caused by ignoring random,three-stage DEA model was studied to remove environmental factors and random effects.On the foundation of this model was two-oriented agriculture comprehensive production efficiency of 14 cities were estimated in Hunan Province in 2008,and brown forth corresponding policy proposals to promote agricultural development.展开更多
Allium tuberosum Roxb is very popular for its function of stomach-strengthening,spirit-freshening,yang-warming and kidney essencereplenishing,however,its seeds appear to low bud,irregular sapling and other side effect...Allium tuberosum Roxb is very popular for its function of stomach-strengthening,spirit-freshening,yang-warming and kidney essencereplenishing,however,its seeds appear to low bud,irregular sapling and other side effects,which result in low yield and poor quality.This paper analyzed effects of PEG6000 concentration on budding percentage in detail.It comes to conclusions that budding percentage of No.1 seed increases from 71% to 92% on the basis of 36 h treatment by 20% PEG6000 and 24 h water-drying,that of No.3 seed ranges from 30.4% to 53.4% on the basis of the same treatment by 10% PEG6000.展开更多
Agricultural mechanization is to the use advanced and applicable agricultural machinery and equipment in agriculture to improve agricultural production conditions, and continuously improve the production technology an...Agricultural mechanization is to the use advanced and applicable agricultural machinery and equipment in agriculture to improve agricultural production conditions, and continuously improve the production technology and economic, ecological processes of agriculture. The level of agricultural science and technology depends largely on the degree of agricultural mechanization promotion. An agricultural country level depends on the degree of modern agricultural mechanization level of the application, and the era of mechanization in agriculture can not only save a lot of labor, reduce labor intensity, but also improve labor efficiency, and enhance resistance to natural disasters. The importance of agricultural mechanization technology in the scientific and technological achievements in agriculture is visible.展开更多
基金Supported by Chongqing Natural Science Foundation~~
文摘[ Objective] The aim was to study method for extracting DNA from pteridophyta and provide basis for further study on genetic diversity and taxonomy. [ Method] By changing the dosage of reagent and operating method, CTAB method for extracting DNA was improved. [ Result] The results showed that the improved CTAB method could extract high-quality DNA from pteridophyta. [ Conclusion] The study improved method for extracting DNA from pteridophyta.
文摘Fenlong green ecological agriculture technology （Fenlong technology）, a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation technique by the Ministry of Agriculture of China. It replaces the traditional plowshare with spiral drill, and its tilth depth is twice deeper than that by tractor tilthing. It also extends soil nutrient, moisture, oxygen and microorganism, the so-called ＂Four pools＂. Soil nutrient, oxygen, microorganism, light and rainfall use ratio is increased by 10%-100%, creating a platform for natural increase of more than 10% of crop yield. Its application to over 20 kinds of crops in 21 provinces has proved that the yield increases 10-30% with quality enhancing 5% and double water retaining capacity but no more input. When the application area of Fenlong could reach 67 million hm2, the amount of fertilizer can be reduced by 40-50 billion kg, saving 120-150 billion Yuan. In this paper, we put forward the strategy of ＂4＋1＂ （arable, saline-alkali soil, grasslands, Sponge City ＋ rivers） green development in China, and deepened the Fenlong cultivated tilled layer from 16.5 cm to 35 cm for 67 million hm2 arable land, ridged 13.3 million hm2 of saline-alkali soil for 35 cm, and also 35 cm for 67 million hm2 degraded steppe, which could have the following 3 effects： first, the 147 million hm2 of land with Fenlong cultivation could increase loosing soil to 315.491 billion m3, in＊ creasing by 159.26% for 120 million hm2 of arable land with the average tilled layer of 16.5 cm, which has loosing soil of only 198.1 billion m3, that is, the space of the land increases 1.6 times. Second, every hectare of plowland could store up to 450 m3/hm2 of natural rainfall, and the unused 60 m3 of saline-alkali soil and grasslands could store water of 102 billion m3, showing an increase of over 88.89% for the current plowland storage of 54 billion m3 at now, that is, double the natural rainfall storage capacity. Third, the two multiple increase of natural resources application can bring trillions of resource activation, environmental cleaning, food security, citizens, health, economic, ecological and social benefits, and makes the Chinese nation move forward in green development. Its application in ＂big scientific research＂ and ＂One Belt And One Road＂ will contribute Chinese strength to the world.
文摘To understand the high yield and efficiency technique in hybrid rice Zhongzheyou No. 1, we conducted the correlation analysis, regression analysis and path analysis of hybrid rice Zhongzheyou No. 1 based on the data of its ear, grain and weight at different yield levels. From this study, we put forward the high yield and efficiency technique in Zhongzheyou No. 1 .. on the basis of certain effective ear number, filled grains per ear should be mainly targeted with a consideration to 1 000-grain weight.
基金Supported by the National Natural Science Foundation of China(30671346)Beijing Natural Science Foundation (6072010)Beijing Municipal Science and Technology Plan Project (D0706005000091)~~
文摘[Objective] The aim of this study was to investigate the preparation method and amplification system of antagonistic streptomyces DNA templates based on AFLP assays, and also provide a basis for the application of AFLP technology in the analysis of streptomyces or even actinomyces. [Method] The DNAs were extracted by the modified CTAB method and amplified by the Pst Ⅰ/Mse Ⅰ AFLP kit and its reaction system. The amplified products were analyzed by the denatured polyacrylamide gel electrophoresis. [Result] The genomic DNAs of ten antagonistic strains of Streptomyces were extracted and tested. The result of 0.8% agarose gel electrophoresis showed that the major DNA bands were clear without degradation and RNA residue, with the fragment sizes ranging from 37.64 to 40.86 Kb. By ultraviolet spectrophotometry, the OD260/OD280 values varying from 1.625 to 1.833 were obtained. Furthermore, the agarose gel electrophoresis of DNA products digested by Pst Ⅰ/Mse Ⅰ presented the dispersed fluorescent long band, which indicated that the enzymatic hydrolysis was fully carried out. The amplified bands of DNA templates by the screened three pairs of primers were clear with rich polymorphism. [Conclusion] The preparation method and amplification system of DNA template established in this study can be used in the AFLP analysis of Streptomyces.
文摘Two-oriented agriculture was a complex organism coupling production,economics,society and ecology.Its development process was affected by various factors such as producers,nature,society,etc.In order to overcome measurement error of traditional data envelopment analysis caused by ignoring random,three-stage DEA model was studied to remove environmental factors and random effects.On the foundation of this model was two-oriented agriculture comprehensive production efficiency of 14 cities were estimated in Hunan Province in 2008,and brown forth corresponding policy proposals to promote agricultural development.
文摘Allium tuberosum Roxb is very popular for its function of stomach-strengthening,spirit-freshening,yang-warming and kidney essencereplenishing,however,its seeds appear to low bud,irregular sapling and other side effects,which result in low yield and poor quality.This paper analyzed effects of PEG6000 concentration on budding percentage in detail.It comes to conclusions that budding percentage of No.1 seed increases from 71% to 92% on the basis of 36 h treatment by 20% PEG6000 and 24 h water-drying,that of No.3 seed ranges from 30.4% to 53.4% on the basis of the same treatment by 10% PEG6000.
文摘Agricultural mechanization is to the use advanced and applicable agricultural machinery and equipment in agriculture to improve agricultural production conditions, and continuously improve the production technology and economic, ecological processes of agriculture. The level of agricultural science and technology depends largely on the degree of agricultural mechanization promotion. An agricultural country level depends on the degree of modern agricultural mechanization level of the application, and the era of mechanization in agriculture can not only save a lot of labor, reduce labor intensity, but also improve labor efficiency, and enhance resistance to natural disasters. The importance of agricultural mechanization technology in the scientific and technological achievements in agriculture is visible.
