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冬季河流中抗生素抗性基因和微生物群落相关性研究 被引量:4
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作者 罗晓 张文丽 +4 位作者 许曼 张迎 蒋永丰 何磊 钟为章 《环境科学与技术》 CAS CSCD 北大核心 2019年第5期20-26,共7页
为分析冬季河流中tetA、tetB、sul1和sul2抗性基因的分布特征与微生物群落的相关性,用Miseq高通量测序分析和荧光定量PCR技术进行。冬季河流地表水中抗性基因丰度比沉积物中的高2个数量级,磺胺类抗性基因丰度比四环素类的高1个数量级。... 为分析冬季河流中tetA、tetB、sul1和sul2抗性基因的分布特征与微生物群落的相关性,用Miseq高通量测序分析和荧光定量PCR技术进行。冬季河流地表水中抗性基因丰度比沉积物中的高2个数量级,磺胺类抗性基因丰度比四环素类的高1个数量级。4种抗性基因总丰度变化范围为3.65×10~2~7.94×10~7copies/L。河流中Proteobacteria和Bacteroidetes为绝对优势菌群,河流地表水与沉积物中优势菌群略有不同。4种抗性基因对样本的影响较大,微生物群落与抗性基因之间相关性较大。4种抗性基因与河流地表水样本均呈正相关,沉积物中仅有sul1与WS1、QS1呈正相关,其他均呈负相关。抗性基因的传播仅与特定的菌群有关,大多数菌属是影响抗性基因分布丰度的主要因素,且对四环素类抗性基因影响的菌群较多。 展开更多
关键词 冬季河流 抗性基因 荧光定量PCR 高通量测序 微生物群落结构
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冬季河流流量测验精度分析 被引量:1
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作者 王振英 《水利科技与经济》 2012年第6期78-79,共2页
冬季河流流量测验比较复杂,河流冰情对流量测验的精度影响比较大,而西北地区冬季时间漫长,冰情流量测验时间一般可达3~4个月,因此,正确掌握冬季流量测验方法,不仅能提高冬季流量测验的精度,还能极大地减轻测验人员的工作量,通过一号站... 冬季河流流量测验比较复杂,河流冰情对流量测验的精度影响比较大,而西北地区冬季时间漫长,冰情流量测验时间一般可达3~4个月,因此,正确掌握冬季流量测验方法,不仅能提高冬季流量测验的精度,还能极大地减轻测验人员的工作量,通过一号站点的冬季流量试验资料,采用多点法与一点法流量资料进行对比分析,提出冬季流量测验的较好方法。 展开更多
关键词 流量测验 精测法 常测法 对比分析 冬季河流
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Interdecadal change of the connection between winter North Pacific Oscillation and summer precipitation in the Huaihe River valley 被引量:6
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作者 ZHOU BoTao XIA DongDong 《Science China Earth Sciences》 SCIE EI CAS 2012年第12期2049-2057,共9页
The relationship between the winter North Pacific Oscillation (NPO) and the following summer precipitation in the Huaihe River valley before and after the mid-1970s is investigated by using the Chinese 160-station pre... The relationship between the winter North Pacific Oscillation (NPO) and the following summer precipitation in the Huaihe River valley before and after the mid-1970s is investigated by using the Chinese 160-station precipitation data and NCEP/NCAR reanalysis data from 1951 to 2008.It is found that their linkage appears to have an apparent interdecadal variation.Before the mid-1970s, there was a prominent out-of-phase relationship between the winter NPO and the summer precipitation in the Huaihe River valley.However, such a relationship is significantly weakened afterwards.The change of atmospheric circulations related to the winter NPO before and after the mid-1970s is further addressed.Before the mid-1970s, a strong (weak) winter NPO was followed by the summer situations with anomalous low-level cyclonic (anticyclonic) circulation over the western Pacific and descending (ascending) over the Huaihe River valley.Meanwhile, the water vapor transporting to the Huaihe River valley was reduced (enhanced).These conditions are unfavorable (favorable) for the precipitation occurring in the Huaihe River valley, and thus the local precipitation was decreased (increased).After the mid-1970s, however, the impact of winter NPO on the summer atmospheric circulation system associated with the rainfall in the Huaihe River valley becomes diluted, thereby weakening its linkage to the summer precipitation in the Huaihe River valley. 展开更多
关键词 precipitation in the Huaihe River valley North Pacific Oscillation interdecadal change atmospheric circulation
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