[Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ...[Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ultraviolet irradiation in short time. [Method] The winter wheat taken as materials were treated with 15 and 30 pW/cm^2 UV-B radiation, then the physiological indexes such as pigment content and photosynthetic rate were determined. [ Results] The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 pW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UVB treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2 h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious. [ Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose. With the increase of treatment time, the damage was alleviated; besides, the result was not similar to that of UV-B radiation in long time.展开更多
Detailed studies on population structure and regeneration ecology ofllex khasiana were carried out at five representative natural populations i.e. Mylliem （Population - I）, Upper Shillong （Population - II）, Shillo...Detailed studies on population structure and regeneration ecology ofllex khasiana were carried out at five representative natural populations i.e. Mylliem （Population - I）, Upper Shillong （Population - II）, Shillong Peak （Population -III）, Laitkor （Population - IV） and Nongpiyur （Population - V） to probe into the responsible affecting factors for its population structure and regeneration ability in these populations. Experimental observation under three controlled light conditions （full sunlight （100%）, intermediate sunlight （50%） and low sunlight （30%）） showed that the growth status and survival rates of seedlings under intermediate and low light were better than those under the high light condition. The density-diameter distribution in population of adult trees （〉Scm dbh） exhibited a typical character of a regenerating population. A higher seedling mortality rate in natural populations of I. khasiana was most probably due to increase in light intensity following vegetation destruction and other anthropogenic disturbances that cause opening of forest canopy. The findings of the present study would be of immense value in formulating appropriate conservation measures for the species.展开更多
[Objective] The paper was to study the changes of protein content and component in leaves of Toona Sinensis under low-temperature stress.[Method] Potted experiment was adopted,and SDS-PAGE electrophoresis was also use...[Objective] The paper was to study the changes of protein content and component in leaves of Toona Sinensis under low-temperature stress.[Method] Potted experiment was adopted,and SDS-PAGE electrophoresis was also used to analyze the dynamic changes of protein in T.Sinensis leaves.[Result] Low temperature stress could change the content and component in leaves of T.Sinensis,but different provenances had different performance under different temperatures,so did the same provenance under different stress periods.SDS-PAGE electrophoresis results indicated that the change law of provenances of Xixia of Henan and Nanjing of Jiangsu with stronger cold resistance was similar,showing the change trend of "increase-decrease-increase".Protein was greatly expressed after stress for 1 d,the color of band became darker;the content gradually decreased in the following second and third day,and the color of bands was lighter;the content began to increase at the forth day.Two provenances induced the specific proteins with molecular weights of 27.6 and 22.5 kD,respectively.The soluble protein content of provenance of Xiapu of Fujian with relatively weak cold resistance was gradually increased,but no new protein bands were induced.The changes of protein band color of various provenances in SDS-PAGE electrophoresis was basically consistent with the changes of protein content,the provenance with stronger cold resistance could induce the production of specific proteins.[Conclusion] The study provided theoretical basis for under the molecular mechanism of plant cold resistance,which had great significance in theory and practice.展开更多
In an organic nursery, various bioproducts were used to stimulate plant growth： Fertigo （granulated bovine manure）, Micosat, Humus UP （humic acids microbiologically enriched）, Humus Active ＋ Aktywit PM （benefic...In an organic nursery, various bioproducts were used to stimulate plant growth： Fertigo （granulated bovine manure）, Micosat, Humus UP （humic acids microbiologically enriched）, Humus Active ＋ Aktywit PM （beneficial microorganisms）, BioFeed Amin, BioFeed Quality, Tytanit, and Vinassa. One of the aims of the study was to determine the effect of these products on the ability of grafted buds of apple and sour cherry cultivars to survive the winter. Losses resulting from the freezing of buds during winter dormancy were calculated as the difference between the number of buds deemed to have taken in autumn and the number of live buds that began to develop properly in the spring of the following year. In autumn, the number of maiden trees obtained in each fertilization combination was also determined in the same nursery. The greatest tendency to die in winter was shown by apple and sour cherry buds in those combinations in which the rootstocks were supplied with a mineral fertilizer with NPK components, and the smallest where humic preparations （so-called vermiculites） were used. The type of fertilizer had an indirect influence not only on the number of grafted buds that died in winter as a result of freezing, but also on the final number of maiden trees obtained from a given area of the nursery.展开更多
基金Supported by Natural Science Foundation of China(Grant:30771156)Student Technology New Ideas Project of Liaocheng University(Grant:SRT08111SM2)~~
文摘[Objective] studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of winter wheat seedlings was to provide reference for the mechanism of plant response to ultraviolet irradiation in short time. [Method] The winter wheat taken as materials were treated with 15 and 30 pW/cm^2 UV-B radiation, then the physiological indexes such as pigment content and photosynthetic rate were determined. [ Results] The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 pW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UVB treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2 h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious. [ Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose. With the increase of treatment time, the damage was alleviated; besides, the result was not similar to that of UV-B radiation in long time.
文摘Detailed studies on population structure and regeneration ecology ofllex khasiana were carried out at five representative natural populations i.e. Mylliem （Population - I）, Upper Shillong （Population - II）, Shillong Peak （Population -III）, Laitkor （Population - IV） and Nongpiyur （Population - V） to probe into the responsible affecting factors for its population structure and regeneration ability in these populations. Experimental observation under three controlled light conditions （full sunlight （100%）, intermediate sunlight （50%） and low sunlight （30%）） showed that the growth status and survival rates of seedlings under intermediate and low light were better than those under the high light condition. The density-diameter distribution in population of adult trees （〉Scm dbh） exhibited a typical character of a regenerating population. A higher seedling mortality rate in natural populations of I. khasiana was most probably due to increase in light intensity following vegetation destruction and other anthropogenic disturbances that cause opening of forest canopy. The findings of the present study would be of immense value in formulating appropriate conservation measures for the species.
基金Supported by National Natural Science Foundation of China(30872055)Key Scientific and Technological Project of Henan(092102110095)
文摘[Objective] The paper was to study the changes of protein content and component in leaves of Toona Sinensis under low-temperature stress.[Method] Potted experiment was adopted,and SDS-PAGE electrophoresis was also used to analyze the dynamic changes of protein in T.Sinensis leaves.[Result] Low temperature stress could change the content and component in leaves of T.Sinensis,but different provenances had different performance under different temperatures,so did the same provenance under different stress periods.SDS-PAGE electrophoresis results indicated that the change law of provenances of Xixia of Henan and Nanjing of Jiangsu with stronger cold resistance was similar,showing the change trend of "increase-decrease-increase".Protein was greatly expressed after stress for 1 d,the color of band became darker;the content gradually decreased in the following second and third day,and the color of bands was lighter;the content began to increase at the forth day.Two provenances induced the specific proteins with molecular weights of 27.6 and 22.5 kD,respectively.The soluble protein content of provenance of Xiapu of Fujian with relatively weak cold resistance was gradually increased,but no new protein bands were induced.The changes of protein band color of various provenances in SDS-PAGE electrophoresis was basically consistent with the changes of protein content,the provenance with stronger cold resistance could induce the production of specific proteins.[Conclusion] The study provided theoretical basis for under the molecular mechanism of plant cold resistance,which had great significance in theory and practice.
文摘In an organic nursery, various bioproducts were used to stimulate plant growth： Fertigo （granulated bovine manure）, Micosat, Humus UP （humic acids microbiologically enriched）, Humus Active ＋ Aktywit PM （beneficial microorganisms）, BioFeed Amin, BioFeed Quality, Tytanit, and Vinassa. One of the aims of the study was to determine the effect of these products on the ability of grafted buds of apple and sour cherry cultivars to survive the winter. Losses resulting from the freezing of buds during winter dormancy were calculated as the difference between the number of buds deemed to have taken in autumn and the number of live buds that began to develop properly in the spring of the following year. In autumn, the number of maiden trees obtained in each fertilization combination was also determined in the same nursery. The greatest tendency to die in winter was shown by apple and sour cherry buds in those combinations in which the rootstocks were supplied with a mineral fertilizer with NPK components, and the smallest where humic preparations （so-called vermiculites） were used. The type of fertilizer had an indirect influence not only on the number of grafted buds that died in winter as a result of freezing, but also on the final number of maiden trees obtained from a given area of the nursery.
