[Objective ] The study aimed to provide a theoretical basis for the clinical application of collagen peptide from Cyanea nozakii. [ Method] After acute toxicity test on mice, collagen peptide from C. nozakii were give...[Objective ] The study aimed to provide a theoretical basis for the clinical application of collagen peptide from Cyanea nozakii. [ Method] After acute toxicity test on mice, collagen peptide from C. nozakii were given to mice by continuous intragastric administration for 30 d at the doses of 25, 50, 100 mg/kg, and then the phagocytosis of macrophage, delayed type hypersensitivity （DTH） and serum hemolysin level were determined. [ Result] Collagen peptide from C. nozakii was atoxic or low toxic, and the three immune indices of experimental groups were signifi- canUy higher than those of the control group （treated with same volume of normal saline） at 0.05 or 0.01 level. E Conclusion] Collagen peptide from C. nozakii has a certain immunopotentiation.展开更多
The experiment was designed to study the preparation, cryopreservation and culture of calf spermatogonial stem cells in order to provide some data for theoretical research and practical use of calf spermatogonial stem...The experiment was designed to study the preparation, cryopreservation and culture of calf spermatogonial stem cells in order to provide some data for theoretical research and practical use of calf spermatogonial stem cells. As for enzymolysis of testis tissue, two digestive method A and B. The cryopreservation cooling procedure was that spermatogonial stem cells was equilibrated at 4℃ for lh, at -20℃ for lh, at -40℃ for 2h, at -80℃ overnight and then stored in liquid nitrogen. The different concentration of three eryoprotectants were compared. The results indicated that both method A and method B achieved the same high motility rates of cells with method B needing time longer, calf spermatogonial stem cells could be well cryopreserved by the stage cooling procedure. Satisfactory cryopreservative results were gotten by adding 10% DMSO or 10% EG to cryopreservative medium, the former was better and adding sucrose could improved cryopreservative effect. The culture behaviors of spermatogonial stem cells kept the same before and after cryopreservation. It was concluded that an effective method was verified for preparation, cryopreservation and culture of new calf spermatogonial stem cells.展开更多
Cryosurgery is an effective way of curing many diseases including tumors and cancers. It can be applied using a variety of systems and cryogens. Cheap, convenient, reliable equipment still needs to be developed so tha...Cryosurgery is an effective way of curing many diseases including tumors and cancers. It can be applied using a variety of systems and cryogens. Cheap, convenient, reliable equipment still needs to be developed so that cryotherapy may be accepted by surgeons and hospitals. This paper presents a cryosurgery apparatus that utilizes an auto-cascade refrigeration system. Refrigerant mixture R50/R23/R600a was selected as the working fluid. The mixture composition was altered to achieve lower temperatures and higher capacity. The lowest temperature at the cryoprobe could be as low as -100℃, and 8 W refrigeration capacity could be obtained at -80 ℃. An ice ball of 11.6 mm diameter could be formed when the cryoprobe was immersed in a water bath at 37 ℃.展开更多
Graphene sponge(GS) is a porous 3D structure of graphene. Although hydrothermal reduction, chemical vapor deposition, solution reduction and high temperature annealing could be used for the preparation of GS, the size...Graphene sponge(GS) is a porous 3D structure of graphene. Although hydrothermal reduction, chemical vapor deposition, solution reduction and high temperature annealing could be used for the preparation of GS, the size and shape cannot be well controlled. Herein, we reported a facile method to prepare GS under mild condition in a size and shape controllable way. Graphene oxide was lyophilized to form the spongy structure and reduced by steamy hydrazine hydrate to produce GS. The size and shape of GS prepared were nearly identical to that of the container. The reduction degree of GS could be regulated by the reduction temperature and time.展开更多
文摘[Objective ] The study aimed to provide a theoretical basis for the clinical application of collagen peptide from Cyanea nozakii. [ Method] After acute toxicity test on mice, collagen peptide from C. nozakii were given to mice by continuous intragastric administration for 30 d at the doses of 25, 50, 100 mg/kg, and then the phagocytosis of macrophage, delayed type hypersensitivity （DTH） and serum hemolysin level were determined. [ Result] Collagen peptide from C. nozakii was atoxic or low toxic, and the three immune indices of experimental groups were signifi- canUy higher than those of the control group （treated with same volume of normal saline） at 0.05 or 0.01 level. E Conclusion] Collagen peptide from C. nozakii has a certain immunopotentiation.
文摘The experiment was designed to study the preparation, cryopreservation and culture of calf spermatogonial stem cells in order to provide some data for theoretical research and practical use of calf spermatogonial stem cells. As for enzymolysis of testis tissue, two digestive method A and B. The cryopreservation cooling procedure was that spermatogonial stem cells was equilibrated at 4℃ for lh, at -20℃ for lh, at -40℃ for 2h, at -80℃ overnight and then stored in liquid nitrogen. The different concentration of three eryoprotectants were compared. The results indicated that both method A and method B achieved the same high motility rates of cells with method B needing time longer, calf spermatogonial stem cells could be well cryopreserved by the stage cooling procedure. Satisfactory cryopreservative results were gotten by adding 10% DMSO or 10% EG to cryopreservative medium, the former was better and adding sucrose could improved cryopreservative effect. The culture behaviors of spermatogonial stem cells kept the same before and after cryopreservation. It was concluded that an effective method was verified for preparation, cryopreservation and culture of new calf spermatogonial stem cells.
基金Project (No.50606032) supported by the National Natural Science Foundation of China
文摘Cryosurgery is an effective way of curing many diseases including tumors and cancers. It can be applied using a variety of systems and cryogens. Cheap, convenient, reliable equipment still needs to be developed so that cryotherapy may be accepted by surgeons and hospitals. This paper presents a cryosurgery apparatus that utilizes an auto-cascade refrigeration system. Refrigerant mixture R50/R23/R600a was selected as the working fluid. The mixture composition was altered to achieve lower temperatures and higher capacity. The lowest temperature at the cryoprobe could be as low as -100℃, and 8 W refrigeration capacity could be obtained at -80 ℃. An ice ball of 11.6 mm diameter could be formed when the cryoprobe was immersed in a water bath at 37 ℃.
基金financial support from the China Natural Science Foundation (No. 201307101)the Science and Technology Department of Sichuan Province (No. 20134FZ0060)+2 种基金Top-notch Young Talents Program of Chinathe Project of Postgraduate Degree ConstructionSouthwest University for Nationalities (No. 2015XWD-S0703)
文摘Graphene sponge(GS) is a porous 3D structure of graphene. Although hydrothermal reduction, chemical vapor deposition, solution reduction and high temperature annealing could be used for the preparation of GS, the size and shape cannot be well controlled. Herein, we reported a facile method to prepare GS under mild condition in a size and shape controllable way. Graphene oxide was lyophilized to form the spongy structure and reduced by steamy hydrazine hydrate to produce GS. The size and shape of GS prepared were nearly identical to that of the container. The reduction degree of GS could be regulated by the reduction temperature and time.
