2020年1月21日,全球顶级杂志《自然》(Nature)发表综述文章,“Technologies to watch in 2020”,请七位行业思想领袖预测技术发展将在今年产生的重大影响。清华大学生命科学学院王宏伟教授:更好地冷冻电镜样品在两到三年中透射式冷冻电...2020年1月21日,全球顶级杂志《自然》(Nature)发表综述文章,“Technologies to watch in 2020”,请七位行业思想领袖预测技术发展将在今年产生的重大影响。清华大学生命科学学院王宏伟教授:更好地冷冻电镜样品在两到三年中透射式冷冻电子显微镜(cryogenic electron microscopy,cryo-EM)将成为解密大分子结构的最强大工具。这些结构对于理解生化机制和药物开发至关重要,而更有效地解析它们的方法可以加快此类工作。展开更多
The structural information of functionally important mac- romolecular assemblies is a key to molecular biology and is of great interest to structural biologists. Cryo-electron mi- croscopy （cryo-EM）, particularly th...The structural information of functionally important mac- romolecular assemblies is a key to molecular biology and is of great interest to structural biologists. Cryo-electron mi- croscopy （cryo-EM）, particularly the single particle analysis （SPA）, has been regarded as one of the three primary tech- niques for structure determination, together with X-ray crystallography and nuclear magnetic resonance （NMR）. For single particle cryo-EM, a tiny amount （~3 μL） of puri- fied biological samples at a concentration around 0.1-2 mg mL-1 are placed onto an EM grid, blotted with a filter paper and rapidly frozen into a thin layer of vitreous ice. The EM grid with frozen samples is then transferred to a transmis- sion electron microscope for imaging.展开更多
文摘2020年1月21日,全球顶级杂志《自然》(Nature)发表综述文章,“Technologies to watch in 2020”,请七位行业思想领袖预测技术发展将在今年产生的重大影响。清华大学生命科学学院王宏伟教授:更好地冷冻电镜样品在两到三年中透射式冷冻电子显微镜(cryogenic electron microscopy,cryo-EM)将成为解密大分子结构的最强大工具。这些结构对于理解生化机制和药物开发至关重要,而更有效地解析它们的方法可以加快此类工作。
文摘The structural information of functionally important mac- romolecular assemblies is a key to molecular biology and is of great interest to structural biologists. Cryo-electron mi- croscopy （cryo-EM）, particularly the single particle analysis （SPA）, has been regarded as one of the three primary tech- niques for structure determination, together with X-ray crystallography and nuclear magnetic resonance （NMR）. For single particle cryo-EM, a tiny amount （~3 μL） of puri- fied biological samples at a concentration around 0.1-2 mg mL-1 are placed onto an EM grid, blotted with a filter paper and rapidly frozen into a thin layer of vitreous ice. The EM grid with frozen samples is then transferred to a transmis- sion electron microscope for imaging.
