[Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. [Method] The Abies beshanzuensis M.H.W...[Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. [Method] The Abies beshanzuensis M.H.Wu were treated by water (CK), 10 mg/L ABT+ water, 10 mg/L IAA+ water, 10 mg/L ABT+ hoagland solution, 10 mg/L IAA+ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were investigated and analyzed. [Result] ABT and IAA had obvious influences on callus induction, rooting rate and the number of root of Abies beshanzuensis M.H.Wu by water culture, so they were suitable to be used in water propagation of Abies beshanzuensis M.H.Wu. The treatments of phytohormones had no regular influences on the longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M.H.Wu at firstly stage, but they generated influence on root growth after rooting. [Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M.H.Wu, which could make it out of endangerment situation quickly.展开更多
The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height...The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs.展开更多
基金Supported by Science and Technology Plan of Zhejiang Province(2005C32036)National Natural Science Foundation of China(30700644)~~
文摘[Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. [Method] The Abies beshanzuensis M.H.Wu were treated by water (CK), 10 mg/L ABT+ water, 10 mg/L IAA+ water, 10 mg/L ABT+ hoagland solution, 10 mg/L IAA+ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were investigated and analyzed. [Result] ABT and IAA had obvious influences on callus induction, rooting rate and the number of root of Abies beshanzuensis M.H.Wu by water culture, so they were suitable to be used in water propagation of Abies beshanzuensis M.H.Wu. The treatments of phytohormones had no regular influences on the longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M.H.Wu at firstly stage, but they generated influence on root growth after rooting. [Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M.H.Wu, which could make it out of endangerment situation quickly.
文摘The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs.
