The quantitative trait loci （QTLs） for the seedling vigor traits under 12℃ cold water irrigation, such as the seedling height, the seedling fresh weight, the seedling dry weight, and their cold response index, were...The quantitative trait loci （QTLs） for the seedling vigor traits under 12℃ cold water irrigation, such as the seedling height, the seedling fresh weight, the seedling dry weight, and their cold response index, were identified using an F2：3 population including 200 individuals and lines derived from a cross of indica and japonica ＂Milyang 23/Jileng 1＂ with microsatellite markers. All seedling vigor traits exhibited a continuous distribution near normal in F3 lines; these traits were quantitative traits controlled by multiple genes. Twelve QTLs conferring the seedling vigor traits under cold water irrigation were detected on chromosomes 1, 2, 7, 8, and 12, which explained the observed phenotypic variance from 5.2% to 17.9%. Among them, qCSH2 and qCSH12 were located in RM262-RM263 on chromosome 2 and RM270-RM17 on chromosome 12, respectively, which were associated with the seedling height, qSDW12 and qCSDW1 were located in RM19-RM270 on chromosome 12 and RM 129-RM9 on chromosome 1, respectively, which were correlated with the seedling dry weight and its cold response index, and the explained 16.6%, 17.9%, 15.9%, and 16.2% of the observed phenotypic variation, respectively. These QTLs alleles were derived from cold-tolerant parent Jileng 1; the gene actions of the two front genes showed their additive effect, and the two genes belind showed dominant and over dominant effects, respectively.展开更多
A chilling-repressed cDNA fragment was cloned from rice ( Oryza sativa L.) seedlings by reverse transcription-polymerase chain reaction (RT-PCR) differential display. Database comparison of the cDNA sequence is identi...A chilling-repressed cDNA fragment was cloned from rice ( Oryza sativa L.) seedlings by reverse transcription-polymerase chain reaction (RT-PCR) differential display. Database comparison of the cDNA sequence is identical to atpH encoded by rice chloroplast genome. Northern hybridization showed that its RNA transcript was reduced greatly on the first day and fully inhibited after 2 d of chilling treatment.展开更多
The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height...The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs.展开更多
基金This work was supported by Key Technologies R & D Program (No. 2006BAD13B01) the National Natural Science Foundation of China (No.30070421)+1 种基金973 Foundation (No. 2004CB117201) Cooperative Research between China and Korea (2004-2007).
文摘The quantitative trait loci （QTLs） for the seedling vigor traits under 12℃ cold water irrigation, such as the seedling height, the seedling fresh weight, the seedling dry weight, and their cold response index, were identified using an F2：3 population including 200 individuals and lines derived from a cross of indica and japonica ＂Milyang 23/Jileng 1＂ with microsatellite markers. All seedling vigor traits exhibited a continuous distribution near normal in F3 lines; these traits were quantitative traits controlled by multiple genes. Twelve QTLs conferring the seedling vigor traits under cold water irrigation were detected on chromosomes 1, 2, 7, 8, and 12, which explained the observed phenotypic variance from 5.2% to 17.9%. Among them, qCSH2 and qCSH12 were located in RM262-RM263 on chromosome 2 and RM270-RM17 on chromosome 12, respectively, which were associated with the seedling height, qSDW12 and qCSDW1 were located in RM19-RM270 on chromosome 12 and RM 129-RM9 on chromosome 1, respectively, which were correlated with the seedling dry weight and its cold response index, and the explained 16.6%, 17.9%, 15.9%, and 16.2% of the observed phenotypic variation, respectively. These QTLs alleles were derived from cold-tolerant parent Jileng 1; the gene actions of the two front genes showed their additive effect, and the two genes belind showed dominant and over dominant effects, respectively.
文摘A chilling-repressed cDNA fragment was cloned from rice ( Oryza sativa L.) seedlings by reverse transcription-polymerase chain reaction (RT-PCR) differential display. Database comparison of the cDNA sequence is identical to atpH encoded by rice chloroplast genome. Northern hybridization showed that its RNA transcript was reduced greatly on the first day and fully inhibited after 2 d of chilling treatment.
文摘The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs.
