[Objective] This study was conducted to explain the mechanism of the accumulation characteristics of mineral elements in alpine grassland plants. [Method] Cultivated alpine grassland plant, Poa crymophila, was treated...[Objective] This study was conducted to explain the mechanism of the accumulation characteristics of mineral elements in alpine grassland plants. [Method] Cultivated alpine grassland plant, Poa crymophila, was treated with drought and wa- ter stress, and then the samples were collected and analyzed. [Result] Compared with the control group, under drought and water stress, multiple mineral elements tended to accumulate and increase, and there were significant differences in the contents of Cu, Mn, Ni and P (P〈0.05). [Conclusion] Under drought and water stress, mineral elements in potted Poa crymophila tended to accumulate and in- crease, which is the adaption and response of Poa crymophila to drought and water stress, as well as the re-verification of the starvation effect hypothesis of mineral effects. The starvation effect of mineral elements is one of the endogenic forces for the accumulation and differentiation of mineral elements in grassland plants.展开更多
Based on the urgency of thermal hazard control in deep coal mines,we studied the status of deep thermal damage and cooling technology both at home and abroad,summarized the causes of deep thermal hazard,analysed and c...Based on the urgency of thermal hazard control in deep coal mines,we studied the status of deep thermal damage and cooling technology both at home and abroad,summarized the causes of deep thermal hazard,analysed and compared the control technologies for deep thermal hazards.The results show that the causes of deep thermal damage can be attributed to three aspects,i.e.,climate,geological and mining factors,of which the geological factors are deemed the major reasons for thermal hazards.As well,we compared a number of cooling technologies of domestic and overseas provenance,such as central air conditioning cooling technology,ice cooling technology and water cooling technology,with one other cooling technology,i.e.,the HEMS cooling technology,which has a large and important effect with its unique"pure air"cooling technology,realizes the utilizing of heat resources from underground to the ground.This technology makes use of heat obtained underground;thus the technology can promote low-carbon environmental economic development in coal mines,in order to achieve low- carbon coal production in China.展开更多
Freeze drying and frozen preservation way was used to preserve a moderately thermophilic culture for bioleaching of chalcopyrite concentrate.After preservation of 15 months,the cell viability rate decreases to 22% wit...Freeze drying and frozen preservation way was used to preserve a moderately thermophilic culture for bioleaching of chalcopyrite concentrate.After preservation of 15 months,the cell viability rate decreases to 22% with a cell density of 7×107 mL-1.When the growth time was extended from 8 days to 14 days,cell density would increase in a large scale to about 3×108 mL-1.In the bioleaching experiments,unpreserved and preserved cultures were compared for dissolving chalcopyrite concentrate.Before 44 days,the unpreserved culture can reach a high copper extraction of about 17.4 g/L.While the preserved culture shows a rather low copper extraction,which is only 9.7 g/L.When the bioleaching time was extended to 80 days,copper extraction by preserved culture increases remarkably,and the concentration of copper finally achieves up to 18.3 g/L.On the other hand,copper extraction by the unpreserved culture does not show remarkable increase from 44th to the 80th day,and finally the total copper extraction is 19.8 g/L.As a result,total copper extraction in 80 days by preserved culture approaches that by unpreserved culture and freeze drying and frozen preservation even after 15 months does not bring much decrease of bioleaching ability.展开更多
A mixed culture of bioleaching microorganisms was enriched.Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months,respectively,using PEG-2000 as the protective agent.The ch...A mixed culture of bioleaching microorganisms was enriched.Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months,respectively,using PEG-2000 as the protective agent.The chalcopyrite leaching ability,activity and diversity of the mixed culture before and after preservation were compared.The results showed that the copper extraction rate was 95.7%in chalcopyrite bioleaching within 20 d by the original culture.After cryopreservation for 6 months and 12 months,the copper extraction rate of the mixed culture was 94.9%within 25 d and 93.6%within 35 d,respectively.The cell viability achieved 87%and 41%after being preserved for 6 months and 12 months,respectively.Furthermore,the ecology analysis identified Acidithiobacillus ferrooxidans,Acidithiobacillus caldus,Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture.After cryopreservation for 12 months,the composition of community changed,but the predominant microorganisms still existed.展开更多
Regarding the lack of cold source for underground cooling systems from either mine inflow or return air, field experiments were taken in a high temperature deep coal mine with abundant cold source from surface water. ...Regarding the lack of cold source for underground cooling systems from either mine inflow or return air, field experiments were taken in a high temperature deep coal mine with abundant cold source from surface water. Taking Sanhejian coal mine as an example, this paper introduced the technology scheme of heat disaster governance using surface water cold source. The paper presents the basics of this field experiment at the beginning, following by the design and site layout of the cooling system including the analysis and calculation of cold source. Numerical calculation method is also applied based on the operation parameters to simulate the influence to the surface river ecosystem. The results suggest that the temperature of surface water shall be lower than 34 ℃ after heat exchange, and when more cooling capacities are needed in the future, increasing the water flow is more favorable than increasing the cooling range of water, which is better for the ecological environment protection,展开更多
文摘[Objective] This study was conducted to explain the mechanism of the accumulation characteristics of mineral elements in alpine grassland plants. [Method] Cultivated alpine grassland plant, Poa crymophila, was treated with drought and wa- ter stress, and then the samples were collected and analyzed. [Result] Compared with the control group, under drought and water stress, multiple mineral elements tended to accumulate and increase, and there were significant differences in the contents of Cu, Mn, Ni and P (P〈0.05). [Conclusion] Under drought and water stress, mineral elements in potted Poa crymophila tended to accumulate and in- crease, which is the adaption and response of Poa crymophila to drought and water stress, as well as the re-verification of the starvation effect hypothesis of mineral effects. The starvation effect of mineral elements is one of the endogenic forces for the accumulation and differentiation of mineral elements in grassland plants.
基金Financial support for this project,provided by the New Century Excellent Talent Program of the Ministry of Education(No.NCET- 08-0833)the National Natural Science Foundation of China(No. 41040027)+1 种基金the National Basic Research Program of China(No. 2006CB202200)the Program for Changjiang Scholars and Innovative Research Team in Universities of China(No.IRT0656)
文摘Based on the urgency of thermal hazard control in deep coal mines,we studied the status of deep thermal damage and cooling technology both at home and abroad,summarized the causes of deep thermal hazard,analysed and compared the control technologies for deep thermal hazards.The results show that the causes of deep thermal damage can be attributed to three aspects,i.e.,climate,geological and mining factors,of which the geological factors are deemed the major reasons for thermal hazards.As well,we compared a number of cooling technologies of domestic and overseas provenance,such as central air conditioning cooling technology,ice cooling technology and water cooling technology,with one other cooling technology,i.e.,the HEMS cooling technology,which has a large and important effect with its unique"pure air"cooling technology,realizes the utilizing of heat resources from underground to the ground.This technology makes use of heat obtained underground;thus the technology can promote low-carbon environmental economic development in coal mines,in order to achieve low- carbon coal production in China.
基金Project(2004CB619201) supported by the National Basic Research Program of ChinaProjects(50321402,30428014) supported by the National Natural Science Foundation of ChinaProject(NCET-06-0691) supported by the Program for New Century Excellent Talents in Chinese Universities
文摘Freeze drying and frozen preservation way was used to preserve a moderately thermophilic culture for bioleaching of chalcopyrite concentrate.After preservation of 15 months,the cell viability rate decreases to 22% with a cell density of 7×107 mL-1.When the growth time was extended from 8 days to 14 days,cell density would increase in a large scale to about 3×108 mL-1.In the bioleaching experiments,unpreserved and preserved cultures were compared for dissolving chalcopyrite concentrate.Before 44 days,the unpreserved culture can reach a high copper extraction of about 17.4 g/L.While the preserved culture shows a rather low copper extraction,which is only 9.7 g/L.When the bioleaching time was extended to 80 days,copper extraction by preserved culture increases remarkably,and the concentration of copper finally achieves up to 18.3 g/L.On the other hand,copper extraction by the unpreserved culture does not show remarkable increase from 44th to the 80th day,and finally the total copper extraction is 19.8 g/L.As a result,total copper extraction in 80 days by preserved culture approaches that by unpreserved culture and freeze drying and frozen preservation even after 15 months does not bring much decrease of bioleaching ability.
基金Projects(31470230,51320105006,51604308)supported by the National Natural Science Foundation of ChinaProject(2017RS3003)supported by the Youth Talent Foundation of Hunan Province of China+2 种基金Project(2018JJ2486)supported by the Natural Science Foundation of Hunan Province of ChinaProject(2018WK2012)supported by the Key Research and Development Projects in Hunan Province,ChinaProject(2018zzts767)supported by the Fundamental Research Funds for the Central Universities,China。
文摘A mixed culture of bioleaching microorganisms was enriched.Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months,respectively,using PEG-2000 as the protective agent.The chalcopyrite leaching ability,activity and diversity of the mixed culture before and after preservation were compared.The results showed that the copper extraction rate was 95.7%in chalcopyrite bioleaching within 20 d by the original culture.After cryopreservation for 6 months and 12 months,the copper extraction rate of the mixed culture was 94.9%within 25 d and 93.6%within 35 d,respectively.The cell viability achieved 87%and 41%after being preserved for 6 months and 12 months,respectively.Furthermore,the ecology analysis identified Acidithiobacillus ferrooxidans,Acidithiobacillus caldus,Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture.After cryopreservation for 12 months,the composition of community changed,but the predominant microorganisms still existed.
基金supported by the Key Project of National Natural Science Foundation‘‘Deep Heat Governance and Utilization’’(No.51134005)the Doctoral Fund of Ministry of Education(No.20120023120004)
文摘Regarding the lack of cold source for underground cooling systems from either mine inflow or return air, field experiments were taken in a high temperature deep coal mine with abundant cold source from surface water. Taking Sanhejian coal mine as an example, this paper introduced the technology scheme of heat disaster governance using surface water cold source. The paper presents the basics of this field experiment at the beginning, following by the design and site layout of the cooling system including the analysis and calculation of cold source. Numerical calculation method is also applied based on the operation parameters to simulate the influence to the surface river ecosystem. The results suggest that the temperature of surface water shall be lower than 34 ℃ after heat exchange, and when more cooling capacities are needed in the future, increasing the water flow is more favorable than increasing the cooling range of water, which is better for the ecological environment protection,