The ultrastructure in sperm of wild yak can be used to examined and assess truly and all round the function and fertilization ability of the sperms. The ultrastructure in the sperm of wild yak was investigated under d...The ultrastructure in sperm of wild yak can be used to examined and assess truly and all round the function and fertilization ability of the sperms. The ultrastructure in the sperm of wild yak was investigated under different states, such as fresh semen, frozen semen and capacitation sperm, and its characteristics were described by electron microscope. The sperm consisted of the head, neck and tail, the length was 78.34±7.24 μm. After capaeitation, the acrosome in sperm swelled and vesiculated, there were obvious vesicu]ation in the acrosome. The evagination on the ectoblast of acrosome formed the large vesiculation and inner membrane swelled. The evagination on the ectoblast of acrosome folded and tbrmed catenular vesiculation. The plasma membrane on the tail swelled. The head, neck and tail of the abnormal sperm were abnormal.展开更多
The trade creation and conservation of wild species in Amazon, including collared peccaries （Pecari tajacu）, may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in ...The trade creation and conservation of wild species in Amazon, including collared peccaries （Pecari tajacu）, may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 ℃ over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates （n = 65） were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact （IPM） and sperm morphology. Selected ejaculates （n = 21） were diluted （1：1） in Beltsville Thawing Solution and kept during 48 hours under controlled temperature （17 ℃）. Assessments were made after dilution （TO）, after 24 hours （T24）, and 48 hours after the onset of cooling （T48）. The storage impacted on sperm survival （P 〈 0.05）. Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before.展开更多
文摘The ultrastructure in sperm of wild yak can be used to examined and assess truly and all round the function and fertilization ability of the sperms. The ultrastructure in the sperm of wild yak was investigated under different states, such as fresh semen, frozen semen and capacitation sperm, and its characteristics were described by electron microscope. The sperm consisted of the head, neck and tail, the length was 78.34±7.24 μm. After capaeitation, the acrosome in sperm swelled and vesiculated, there were obvious vesicu]ation in the acrosome. The evagination on the ectoblast of acrosome formed the large vesiculation and inner membrane swelled. The evagination on the ectoblast of acrosome folded and tbrmed catenular vesiculation. The plasma membrane on the tail swelled. The head, neck and tail of the abnormal sperm were abnormal.
文摘The trade creation and conservation of wild species in Amazon, including collared peccaries （Pecari tajacu）, may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 ℃ over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates （n = 65） were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact （IPM） and sperm morphology. Selected ejaculates （n = 21） were diluted （1：1） in Beltsville Thawing Solution and kept during 48 hours under controlled temperature （17 ℃）. Assessments were made after dilution （TO）, after 24 hours （T24）, and 48 hours after the onset of cooling （T48）. The storage impacted on sperm survival （P 〈 0.05）. Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before.
