Objective In order to establish tumour cell line and screen anticancer drugs. Methods We froze cell line in liquid nitrogen container with 10% glycerin. Some of them were kept more than 15 years. Results Cell livabili...Objective In order to establish tumour cell line and screen anticancer drugs. Methods We froze cell line in liquid nitrogen container with 10% glycerin. Some of them were kept more than 15 years. Results Cell livability rate arrived at 80%～90% after anabiosis. Conclusion Our cell store is suitable to offer cell line for scientific research and for complete task of investigation.展开更多
The paper introduced the research progress on the technique of frozen embryo transfer in sheep,illustrated selection of donors and receptors,superovulation,synchronization of estrus,embryo cryopreservation and embryo ...The paper introduced the research progress on the technique of frozen embryo transfer in sheep,illustrated selection of donors and receptors,superovulation,synchronization of estrus,embryo cryopreservation and embryo transplantation. Frozen embryo transfer in sheep is another breakthrough in the high-quality sheep raising,and this technique in China is in its infancy recommendation stage,but it will be comprehensively popularized in the future.展开更多
Cancer is not rare in younger women of reproductive age therefore the preservation of fertility among them has become a significant concern due to aggressive cancer therapy they must undergo.Today different strategies...Cancer is not rare in younger women of reproductive age therefore the preservation of fertility among them has become a significant concern due to aggressive cancer therapy they must undergo.Today different strategies for fertility preservation are available in patients at risk.However,many of these available techniques are still experimental and have limited clinical experience.Under ethical principle,better interdisciplinary cooperation between clinicians and patients,appropriate counseling and further research on cryopreservation and transplantation techniques may enhance their success to preserve fertility of young cancer victims.In this article,the existing and emerging fertility preserving strategies in young cancer female as well as their safety and ethical issues are discussed.展开更多
Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades,emphasizing their importance in various assisted reproductive technologies.Pregnancies and live births ...Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades,emphasizing their importance in various assisted reproductive technologies.Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization.Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos,including preservation of the reproductive potential of patients who may become infertile,establishment of cryopreserved oocyte banks,and transport of oocytes and embryos internationally.However,the success rates are still far lower than those obtained with fresh oocytes and embryos,and there are still obstacles that need to be overcome.In this review,we address the major obstacles in the development of effective cryopreservation techniques.Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement.Furthermore,this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species.展开更多
文摘Objective In order to establish tumour cell line and screen anticancer drugs. Methods We froze cell line in liquid nitrogen container with 10% glycerin. Some of them were kept more than 15 years. Results Cell livability rate arrived at 80%～90% after anabiosis. Conclusion Our cell store is suitable to offer cell line for scientific research and for complete task of investigation.
文摘The paper introduced the research progress on the technique of frozen embryo transfer in sheep,illustrated selection of donors and receptors,superovulation,synchronization of estrus,embryo cryopreservation and embryo transplantation. Frozen embryo transfer in sheep is another breakthrough in the high-quality sheep raising,and this technique in China is in its infancy recommendation stage,but it will be comprehensively popularized in the future.
文摘Cancer is not rare in younger women of reproductive age therefore the preservation of fertility among them has become a significant concern due to aggressive cancer therapy they must undergo.Today different strategies for fertility preservation are available in patients at risk.However,many of these available techniques are still experimental and have limited clinical experience.Under ethical principle,better interdisciplinary cooperation between clinicians and patients,appropriate counseling and further research on cryopreservation and transplantation techniques may enhance their success to preserve fertility of young cancer victims.In this article,the existing and emerging fertility preserving strategies in young cancer female as well as their safety and ethical issues are discussed.
基金supported by grants from the National High Technology Research and Development Program of China(2011AA100602)the National Science and Technology Major Project of China(2013ZX08007-004,2013ZX08008-004)the National Natural Science Foundation of China(31371486)
文摘Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades,emphasizing their importance in various assisted reproductive technologies.Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization.Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos,including preservation of the reproductive potential of patients who may become infertile,establishment of cryopreserved oocyte banks,and transport of oocytes and embryos internationally.However,the success rates are still far lower than those obtained with fresh oocytes and embryos,and there are still obstacles that need to be overcome.In this review,we address the major obstacles in the development of effective cryopreservation techniques.Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement.Furthermore,this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species.
