To investigate the effects of environmental stresses on ascorbic acid content and its redox status, the effects of freezing and drought on ascorbate and dehydro-ascorbate content and activities of four enzymes involve...To investigate the effects of environmental stresses on ascorbic acid content and its redox status, the effects of freezing and drought on ascorbate and dehydro-ascorbate content and activities of four enzymes involved in the ascorbate-glutathione cycle in some conifers were studied. The results showed that both freezing and drought induced the decrease in ascorbate content and the increase in dehydro-ascorbate content. The activities of ascorbate peroxidase (APX) and monodehydro-ascorbate reductase (MDAR) were decreased by freezing stress. At the beginning of exposure to air, water loss from detached needles induced the increase in the activities of APX and MDAR. Further water loss turned to decrease the APX and MDAR activities. The activities of dehydro-ascorbate reductase (DHAR) and glutathione reductase (GR) were not sensitive to changes in temperature and water content of the needles. It is concluded that moderate temperature or water stresses may induce the acclimation and increase in the ability of the H2O2 scavenging system, while strong stresses decrease the ability and induce injury of plant tissues. Correlation between ascorbate content and activities of related enzymes and cold tolerance of conifers were also reported.展开更多
[Objective]The aim was to understand the change characteristics of sugarcane traits and evaluate the cold tolerance of sugarcane varieties under the drought and frost conditions.[Method] The experiment was carried out...[Objective]The aim was to understand the change characteristics of sugarcane traits and evaluate the cold tolerance of sugarcane varieties under the drought and frost conditions.[Method] The experiment was carried out in Ziyuan County,Guangxi Province where the frost occurred often with 21 domestic and abroad sugarcane varieties(elites).[Result] There were significant changes in the brix,green leaf number and photosynthetic rate of sugarcane before and after light frost and decreased more in cold-sensitive varieties.However,the correlation was only significant between the damage rate of stem length and internode,percentage of green leaves after heavy frost in relation to sugarcane brix and brix changes after light frost.Further analysis showed that the evaluation for cold tolerance of sugarcane would be more simple and reliable with traits of the damage rate of stem length and percentage of upper green leaves.The evaluation for varieties indicated that if it was not carried out for cold-tolerance identification in breeding program,the percentage of sugarcane varieties with good cold-tolerance will be lower than30%,and higher than 60% with poor cold-tolerance in subtropical and tropical regions.[Conclusion] This study had provided theoretical basis for the cold-resistant evaluation of sugar cane and the breeding of the varieties of cold-resistant.展开更多
文摘To investigate the effects of environmental stresses on ascorbic acid content and its redox status, the effects of freezing and drought on ascorbate and dehydro-ascorbate content and activities of four enzymes involved in the ascorbate-glutathione cycle in some conifers were studied. The results showed that both freezing and drought induced the decrease in ascorbate content and the increase in dehydro-ascorbate content. The activities of ascorbate peroxidase (APX) and monodehydro-ascorbate reductase (MDAR) were decreased by freezing stress. At the beginning of exposure to air, water loss from detached needles induced the increase in the activities of APX and MDAR. Further water loss turned to decrease the APX and MDAR activities. The activities of dehydro-ascorbate reductase (DHAR) and glutathione reductase (GR) were not sensitive to changes in temperature and water content of the needles. It is concluded that moderate temperature or water stresses may induce the acclimation and increase in the ability of the H2O2 scavenging system, while strong stresses decrease the ability and induce injury of plant tissues. Correlation between ascorbate content and activities of related enzymes and cold tolerance of conifers were also reported.
基金Supported by National Science and Technology Support Program(2008BADB8B01,2007BAD30B02,2007BAD30B05)Modern Agricultural Technology System Special Fund Project(nycytx-024-01-03)Guangxi Scientific and Technological Project(0782004-2,0782004-5)~~
文摘[Objective]The aim was to understand the change characteristics of sugarcane traits and evaluate the cold tolerance of sugarcane varieties under the drought and frost conditions.[Method] The experiment was carried out in Ziyuan County,Guangxi Province where the frost occurred often with 21 domestic and abroad sugarcane varieties(elites).[Result] There were significant changes in the brix,green leaf number and photosynthetic rate of sugarcane before and after light frost and decreased more in cold-sensitive varieties.However,the correlation was only significant between the damage rate of stem length and internode,percentage of green leaves after heavy frost in relation to sugarcane brix and brix changes after light frost.Further analysis showed that the evaluation for cold tolerance of sugarcane would be more simple and reliable with traits of the damage rate of stem length and percentage of upper green leaves.The evaluation for varieties indicated that if it was not carried out for cold-tolerance identification in breeding program,the percentage of sugarcane varieties with good cold-tolerance will be lower than30%,and higher than 60% with poor cold-tolerance in subtropical and tropical regions.[Conclusion] This study had provided theoretical basis for the cold-resistant evaluation of sugar cane and the breeding of the varieties of cold-resistant.