Freeze drying and frozen preservation way was used to preserve a moderately thermophilic culture for bioleaching of chalcopyrite concentrate.After preservation of 15 months,the cell viability rate decreases to 22% wit...Freeze drying and frozen preservation way was used to preserve a moderately thermophilic culture for bioleaching of chalcopyrite concentrate.After preservation of 15 months,the cell viability rate decreases to 22% with a cell density of 7×107 mL-1.When the growth time was extended from 8 days to 14 days,cell density would increase in a large scale to about 3×108 mL-1.In the bioleaching experiments,unpreserved and preserved cultures were compared for dissolving chalcopyrite concentrate.Before 44 days,the unpreserved culture can reach a high copper extraction of about 17.4 g/L.While the preserved culture shows a rather low copper extraction,which is only 9.7 g/L.When the bioleaching time was extended to 80 days,copper extraction by preserved culture increases remarkably,and the concentration of copper finally achieves up to 18.3 g/L.On the other hand,copper extraction by the unpreserved culture does not show remarkable increase from 44th to the 80th day,and finally the total copper extraction is 19.8 g/L.As a result,total copper extraction in 80 days by preserved culture approaches that by unpreserved culture and freeze drying and frozen preservation even after 15 months does not bring much decrease of bioleaching ability.展开更多
Compacted loess is widely used as fills of road embankments in loess regions of northern China.Generally, densely-compacted loess can satisfy the requirements of embankment strength and postconstruction deformation. H...Compacted loess is widely used as fills of road embankments in loess regions of northern China.Generally, densely-compacted loess can satisfy the requirements of embankment strength and postconstruction deformation. However, uneven subsidence, pavement cracks and other related damages can affect the integrity of loess subgrade after several years of operation,and even cause some hazards, especially in North China, where the strong freeze-thaw erosion occurs. In this study, cyclic freeze-thaw tests for both densely and loosely compacted loess samples were performed to determine the variation in engineering properties such as volume, void ratio, collapsible settlement,microstructure, and the related mechanisms were addressed. The experimental results showed that an obvious water migration and redistribution occurred within the samples during freeze-thaw cycles. Ice lenses and fissures could be identified in the upper frozen layers of the samples. After freeze-thaw cycles,the dry densities of the upper layers of samples changed significantly due to strong freeze-thaw erosion. The dry densities decreased for the dense sample and increased for the loose sample. It can be found that dense samples become loose, while loose samples became dense with the increasing number of freeze-thaw cycles. Their related void ratios changed reversely. Both void ratios tended to fall into a certain range, which verified the concept of a residual void ratio proposed by Viklander. The loosening process of densely compacted samples involves the formation of large pores, volume increase and density reduction as well as the related changes in mechanical properties because freeze-thaw cycles may be important contribution to problems of loess road embankments.Adverse effects of freeze-thaw cycles, therefore,should be taken into account in selecting loess parameters for the stability evaluation of road embankment in seasonally frozen ground regions.展开更多
The three-river source region(TRSR), located in the Qinghai-Tibet Plateau in China, suffers from serious freeze-thaw(FT) erosion in China. Considering the unique eco-environment and the driving factors of the FT proce...The three-river source region(TRSR), located in the Qinghai-Tibet Plateau in China, suffers from serious freeze-thaw(FT) erosion in China. Considering the unique eco-environment and the driving factors of the FT process in the TRSR, we introduce the driving force factors of FT erosion(rainfall erosivity and wind field intensity during FT period) and precipitation during the FT period(indicating the phase-changed water content). The objective was to establish an improved evaluation method of FT erosion in the TRSR. The method has good applicability in the study region with an overall precision of 92%. The spatial and temporal changes of FT erosion from 2000 to 2015 are analyzed. Results show that FT erosion is widely distributed in the TRSR, with slight and mild erosion being the most widely distributed, followed by moderate erosion. Among the three sub-regions, the source region of the Yellow River has the slightest erosion intensity, whereas the erosion intensity of the source region of Yangtze River is the most severe. A slight improvement can be observed in the condition of FTerosion over the whole study region from 2000 to 2015. Vegetation coverage is the dominant factor affecting the intensity of FT erosion in the zones with sparse vegetation or bare land, whereas the climate factors play an important role in high vegetation coverage area. Slopes>28° also have a significant effect on the intensity of FT erosion in the zones. The results can provide a scientific basis for the prevention and management of the soil FT erosion in the TRSR.展开更多
基金Project(2004CB619201) supported by the National Basic Research Program of ChinaProjects(50321402,30428014) supported by the National Natural Science Foundation of ChinaProject(NCET-06-0691) supported by the Program for New Century Excellent Talents in Chinese Universities
文摘Freeze drying and frozen preservation way was used to preserve a moderately thermophilic culture for bioleaching of chalcopyrite concentrate.After preservation of 15 months,the cell viability rate decreases to 22% with a cell density of 7×107 mL-1.When the growth time was extended from 8 days to 14 days,cell density would increase in a large scale to about 3×108 mL-1.In the bioleaching experiments,unpreserved and preserved cultures were compared for dissolving chalcopyrite concentrate.Before 44 days,the unpreserved culture can reach a high copper extraction of about 17.4 g/L.While the preserved culture shows a rather low copper extraction,which is only 9.7 g/L.When the bioleaching time was extended to 80 days,copper extraction by preserved culture increases remarkably,and the concentration of copper finally achieves up to 18.3 g/L.On the other hand,copper extraction by the unpreserved culture does not show remarkable increase from 44th to the 80th day,and finally the total copper extraction is 19.8 g/L.As a result,total copper extraction in 80 days by preserved culture approaches that by unpreserved culture and freeze drying and frozen preservation even after 15 months does not bring much decrease of bioleaching ability.
基金supported by the National Key Basic Research Program of China(973 Program)(Grant No.2012CB026106)National Natural Science Foundation of China(No.41672310)+3 种基金the Science and Technology Major Project of Gansu Province(Grant No.143GKDA007)National key research and development program(2016YFC0802103)the West Light Foundation of CAS for Dr.G.Y.Li,Project of the State Key Laboratory of Frozen Soils Engineering of CAS(Grant No.SKLFSE-ZY-16)the STS research project of the Cold and Arid Regions Environmental and Engineering Research Institute(HHS-TSS-STS-1502)
文摘Compacted loess is widely used as fills of road embankments in loess regions of northern China.Generally, densely-compacted loess can satisfy the requirements of embankment strength and postconstruction deformation. However, uneven subsidence, pavement cracks and other related damages can affect the integrity of loess subgrade after several years of operation,and even cause some hazards, especially in North China, where the strong freeze-thaw erosion occurs. In this study, cyclic freeze-thaw tests for both densely and loosely compacted loess samples were performed to determine the variation in engineering properties such as volume, void ratio, collapsible settlement,microstructure, and the related mechanisms were addressed. The experimental results showed that an obvious water migration and redistribution occurred within the samples during freeze-thaw cycles. Ice lenses and fissures could be identified in the upper frozen layers of the samples. After freeze-thaw cycles,the dry densities of the upper layers of samples changed significantly due to strong freeze-thaw erosion. The dry densities decreased for the dense sample and increased for the loose sample. It can be found that dense samples become loose, while loose samples became dense with the increasing number of freeze-thaw cycles. Their related void ratios changed reversely. Both void ratios tended to fall into a certain range, which verified the concept of a residual void ratio proposed by Viklander. The loosening process of densely compacted samples involves the formation of large pores, volume increase and density reduction as well as the related changes in mechanical properties because freeze-thaw cycles may be important contribution to problems of loess road embankments.Adverse effects of freeze-thaw cycles, therefore,should be taken into account in selecting loess parameters for the stability evaluation of road embankment in seasonally frozen ground regions.
基金funded by the Open fund of Key Laboratory for Digital Land and Resources of Jiangxi Province, East China University of Technology (Grant No. DLLJ201709)Open fund of Key Laboratory for National Geographic Census and Monitoring, National Administration of Surveying, Mapping and Geoinformation (Grant No. 2016NGCM02)+2 种基金Open fund of Key Laboratory of Precise Engineering and Industry Surveying (Grant No. PF2015-17)National Administration of Surveying, Mapping and Geoinformation, National Natural Science Foundation of China (Grant Nos. 41501416, 40775019)the Natural Science Foundation of Shandong Province (Grant Nos. ZR2014DL001, ZR2015DL005)
文摘The three-river source region(TRSR), located in the Qinghai-Tibet Plateau in China, suffers from serious freeze-thaw(FT) erosion in China. Considering the unique eco-environment and the driving factors of the FT process in the TRSR, we introduce the driving force factors of FT erosion(rainfall erosivity and wind field intensity during FT period) and precipitation during the FT period(indicating the phase-changed water content). The objective was to establish an improved evaluation method of FT erosion in the TRSR. The method has good applicability in the study region with an overall precision of 92%. The spatial and temporal changes of FT erosion from 2000 to 2015 are analyzed. Results show that FT erosion is widely distributed in the TRSR, with slight and mild erosion being the most widely distributed, followed by moderate erosion. Among the three sub-regions, the source region of the Yellow River has the slightest erosion intensity, whereas the erosion intensity of the source region of Yangtze River is the most severe. A slight improvement can be observed in the condition of FTerosion over the whole study region from 2000 to 2015. Vegetation coverage is the dominant factor affecting the intensity of FT erosion in the zones with sparse vegetation or bare land, whereas the climate factors play an important role in high vegetation coverage area. Slopes>28° also have a significant effect on the intensity of FT erosion in the zones. The results can provide a scientific basis for the prevention and management of the soil FT erosion in the TRSR.
