Objective: To study the physical and chemical properties of an arginine ester hydrolase from the venom of Trimeresurus mucrosqumatus in Hunan province of China.Methods:The arginine ester hydrolase(AEH) was isolated fr...Objective: To study the physical and chemical properties of an arginine ester hydrolase from the venom of Trimeresurus mucrosqumatus in Hunan province of China.Methods:The arginine ester hydrolase(AEH) was isolated from the venom of Chinese Trimeresurus mucrosqumatus by a combination of ion-exchange chromatography on DEAE-Sephadex A-50, CM-Sepharose Cl-6B and gel filtration on Sephadex G-100.Results: The purified protein named TM-AEH,a glycoprotein with carbohydrate content of 0.5% neutral hexose and 0.75% sialic acid,a relative molecular mass of 29.0 kDa,and an isoelectric point(pI) of 5.2. It shares with an extinction coefficient(E 0.1%/cm) of 1.332 at 280 nm,consisted of 225 amino acid residues,and migrated as a band under reduced or non-reduced condition in basic PAGE.TM-AEH was a highly thermostable protein and was stable to pH changes between 5 and 9.The optimum temperature and optimum pH were 55℃ and 8.4 for its catalytic activity respectively,which was inhibited by Fe 3+ and Cu 2+.Conclusion:This protein can exhibit higher BAEE-hydrolysing activity and fibrinogenolytic activity as compared to that of whole venom.展开更多
The desulfurization performance of the UDS solvents was investigated at an industrial side-stream plant and was compared with that of MDEA solvent.A mass transfer performance model was employed for explaining the COS ...The desulfurization performance of the UDS solvents was investigated at an industrial side-stream plant and was compared with that of MDEA solvent.A mass transfer performance model was employed for explaining the COS absorption into different solvents.Meanwhile,the regeneration performance of the UDS solvents was evaluated in side-stream tests.Results indicate that under the conditions covering an absorption temperature of 40℃,a pressure of 8.0 MPa,and a gas to liquid volume ratio(V/L)of around 230,the H2S content in purified gas can be reduced to 4.2 mg/m3 and 0 by using solvents UDS-II and UDS-III,respectively.Moreover,the total sulfur content in both purified gases is less than 80 mg/m3.As a result,the UDS-III solvent shows by 30 percentage points higher in COS removal efficiency than MDEA.In addition,the total volume mass transfer coefficient of UDS solvent is found to be twice higher than that of MDEA.Furthermore,the UDS solvents exhibit satisfactory thermal stability and regeneration performance.展开更多
The purification law of nitrogen in Deyeuxia angustifolia, Carex lasiocarpa and Deyeuxia angustifolia-Carex lasiocarpa combined wetland systems in the Sanjiang Plain, China was studied by field simulation experiment. ...The purification law of nitrogen in Deyeuxia angustifolia, Carex lasiocarpa and Deyeuxia angustifolia-Carex lasiocarpa combined wetland systems in the Sanjiang Plain, China was studied by field simulation experiment. The results indicate that the removal rates of TN, NH4^＋-N and NO3^--N in above three types of wetlands present an obvious logarithm growth trend along with the time. There are evident removal effects for NH4＋-N and NOa--N in water bodies of wetlands after the 30th day of experiment, with the removal rates over 80.0%, but the removal rate of TN is slightly low, being 63.1%-74.3%. NO3 -N is most quickly removed by the combined wetland, and NH4^＋-N by Deyeuxia angustifolia wetland. The removal speeds of TN by the three wetland systems are comparatively slow, of which the Deyeuxia angustifolia wetland is the fastest. In consideration of plant growth season, Deyeuxia angustifolia wetland has much more practical application value in purifying nitrogen. These results can provide references for the study on the purification function of wetlands and the control of non-point source pollution in Northeast China.展开更多
文摘Objective: To study the physical and chemical properties of an arginine ester hydrolase from the venom of Trimeresurus mucrosqumatus in Hunan province of China.Methods:The arginine ester hydrolase(AEH) was isolated from the venom of Chinese Trimeresurus mucrosqumatus by a combination of ion-exchange chromatography on DEAE-Sephadex A-50, CM-Sepharose Cl-6B and gel filtration on Sephadex G-100.Results: The purified protein named TM-AEH,a glycoprotein with carbohydrate content of 0.5% neutral hexose and 0.75% sialic acid,a relative molecular mass of 29.0 kDa,and an isoelectric point(pI) of 5.2. It shares with an extinction coefficient(E 0.1%/cm) of 1.332 at 280 nm,consisted of 225 amino acid residues,and migrated as a band under reduced or non-reduced condition in basic PAGE.TM-AEH was a highly thermostable protein and was stable to pH changes between 5 and 9.The optimum temperature and optimum pH were 55℃ and 8.4 for its catalytic activity respectively,which was inhibited by Fe 3+ and Cu 2+.Conclusion:This protein can exhibit higher BAEE-hydrolysing activity and fibrinogenolytic activity as compared to that of whole venom.
基金financial support from the National Key Science and Technology Project of China (2011ZX05017-005)the Fundamental Research Funds for the Central Universities (No.22A201514010)
文摘The desulfurization performance of the UDS solvents was investigated at an industrial side-stream plant and was compared with that of MDEA solvent.A mass transfer performance model was employed for explaining the COS absorption into different solvents.Meanwhile,the regeneration performance of the UDS solvents was evaluated in side-stream tests.Results indicate that under the conditions covering an absorption temperature of 40℃,a pressure of 8.0 MPa,and a gas to liquid volume ratio(V/L)of around 230,the H2S content in purified gas can be reduced to 4.2 mg/m3 and 0 by using solvents UDS-II and UDS-III,respectively.Moreover,the total sulfur content in both purified gases is less than 80 mg/m3.As a result,the UDS-III solvent shows by 30 percentage points higher in COS removal efficiency than MDEA.In addition,the total volume mass transfer coefficient of UDS solvent is found to be twice higher than that of MDEA.Furthermore,the UDS solvents exhibit satisfactory thermal stability and regeneration performance.
基金Under the auspices of Knowledge Innovation Programes of Chinese Academy of Sciences (No. KZCX2-YW-425-02)National Natural Science Foundation of China (No. 40871049)Key Program of National Natural Science Foundation of China (No. 40830535)
文摘The purification law of nitrogen in Deyeuxia angustifolia, Carex lasiocarpa and Deyeuxia angustifolia-Carex lasiocarpa combined wetland systems in the Sanjiang Plain, China was studied by field simulation experiment. The results indicate that the removal rates of TN, NH4^＋-N and NO3^--N in above three types of wetlands present an obvious logarithm growth trend along with the time. There are evident removal effects for NH4＋-N and NOa--N in water bodies of wetlands after the 30th day of experiment, with the removal rates over 80.0%, but the removal rate of TN is slightly low, being 63.1%-74.3%. NO3 -N is most quickly removed by the combined wetland, and NH4^＋-N by Deyeuxia angustifolia wetland. The removal speeds of TN by the three wetland systems are comparatively slow, of which the Deyeuxia angustifolia wetland is the fastest. In consideration of plant growth season, Deyeuxia angustifolia wetland has much more practical application value in purifying nitrogen. These results can provide references for the study on the purification function of wetlands and the control of non-point source pollution in Northeast China.
