【目的】研究实验室条件下韭蛆Bradysia odoriphaga Yang et Zhang大量饲养的方法以解决试虫不足的问题。【方法】本实验在25℃,相对湿度75%,光周期16L∶8D条件下,通过控制变量法筛选出人工饲料配方,运用两性生命表软件,分析比较人工饲...【目的】研究实验室条件下韭蛆Bradysia odoriphaga Yang et Zhang大量饲养的方法以解决试虫不足的问题。【方法】本实验在25℃,相对湿度75%,光周期16L∶8D条件下,通过控制变量法筛选出人工饲料配方,运用两性生命表软件,分析比较人工饲料与韭菜Allium tuberosum的饲喂效果。【结果】对不同成分和不同含量的8种韭蛆人工饲料配方筛选,结果确定最优的人工饲料配方成分和含量为:平菇粉6 g,韭菜粉5 g,琼脂粉1.25 g,山梨酸0.04 g,苯甲酸0.05 g,酵母粉0.5 g,维生素C 2.5 g和水50 m L。该配方和韭菜饲养效果相当,不存在显著差异(P>0.05)。同时,两性生命表结果表明,人工饲料饲喂的韭蛆平均世代周期23.6800 d,韭菜饲喂的韭蛆世代周期为25.9000 d,两者之间差异显著(P<0.05)。生殖生物学特性分析发现:韭菜和人工饲料饲喂的韭蛆雌成虫净生殖率分别为16.3200和41.1800/d,内禀增长率分别为0.1078和0.1570/d。【结论】筛选的人工饲料和韭菜均可以稳定地完成韭蛆的世代繁殖,且人工饲料饲喂效果略优于韭菜,另外人工饲料较韭菜不但具有低成本省人工的优点,而且可解决韭菜反季节供应和农药残留问题,保证稳定的虫源供给。展开更多
We evaluated the effects of lithium on the survival,growth,and reproduction of D.tibetana in the laboratory.The safe concentration value was 69.3 mg/L.The time to first brood was significant lower for individuals rear...We evaluated the effects of lithium on the survival,growth,and reproduction of D.tibetana in the laboratory.The safe concentration value was 69.3 mg/L.The time to first brood was significant lower for individuals reared in 5 and 10 mg/L(24.4 and 24.0 d,respectively) compared with individuals reared in20,40,or 60 mg/L.Females reared in 5 mg/L lithium produced a mean of 16.5 neonates/brood and had the highest number of broods(3.0±1.95).The rate of egg production(a),the intrinsic rate of increase(r_m),net reproduction rate(R_0),and finite rate of increase(λ) were highest for D.tibetana reared at 5 and 10 mg/L lithium,and the duration of development was shorter than for the remaining groups.The results indicated that rearing in 5-10 mg/L lithium can accelerate the growth and reproduction of D.tibetana.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.30371112,30671625,40776065)
文摘We evaluated the effects of lithium on the survival,growth,and reproduction of D.tibetana in the laboratory.The safe concentration value was 69.3 mg/L.The time to first brood was significant lower for individuals reared in 5 and 10 mg/L(24.4 and 24.0 d,respectively) compared with individuals reared in20,40,or 60 mg/L.Females reared in 5 mg/L lithium produced a mean of 16.5 neonates/brood and had the highest number of broods(3.0±1.95).The rate of egg production(a),the intrinsic rate of increase(r_m),net reproduction rate(R_0),and finite rate of increase(λ) were highest for D.tibetana reared at 5 and 10 mg/L lithium,and the duration of development was shorter than for the remaining groups.The results indicated that rearing in 5-10 mg/L lithium can accelerate the growth and reproduction of D.tibetana.
