[目的]探究凉血活血汤配合常规西药治疗血热型银屑病并发冠心病的疗效,以及对患者皮肤屏障功能、血液流变学的影响。[方法]选取2019年11月至2021年12月血热型银屑病合并冠心病患者88例,随机分为两组,每组44例。对照组予以常规西药治疗,...[目的]探究凉血活血汤配合常规西药治疗血热型银屑病并发冠心病的疗效,以及对患者皮肤屏障功能、血液流变学的影响。[方法]选取2019年11月至2021年12月血热型银屑病合并冠心病患者88例,随机分为两组,每组44例。对照组予以常规西药治疗,观察组予以凉血活血汤配合常规西药治疗。比较两组临床疗效、不良反应及治疗前后皮肤屏障功能、血液流变学指标、血清炎性因子白细胞介素-1(interleukin-1,IL-1)、IL-6、IL-8及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)及皮损面积和严重程度指数(psoriasis area and severity index,PASI)、Barthel指数(Barthel index,BI)。[结果]观察组治疗总有效率(88.64%)较对照组(70.45%)高(P<0.05),观察组不良反应发生率(13.64%)与对照组(9.09%)差异无统计学意义(P>0.05)。治疗2、4周后,观察组角质层含水量、皮脂含量均较对照组高,经表皮水分丢失量、全血高切、低切黏度、红细胞比容、血浆黏度及IL-1、IL-6、IL-8、TNF-α水平较对照组低(P<0.05)。治疗4、12周后,观察组PASI评分较对照组低,BI较对照组高(P<0.05)。[结论]凉血活血汤配合常规西药治疗银屑病合并冠心病患者,可有效减轻机体炎症反应,改善皮肤屏障功能及血液流变学指标,效果颇佳,且安全性高。展开更多
目的:观察凉血活血胶囊对咪喹莫特诱导银屑病样小鼠模型中免疫异常的干预作用。方法:BALB/c雌性小鼠48只,随机分为正常对照组,模型组,凉血活血胶囊高、中、低剂量组和雷公藤多苷组。采用银屑病皮损面积和疾病严重程度(psoriasis area an...目的:观察凉血活血胶囊对咪喹莫特诱导银屑病样小鼠模型中免疫异常的干预作用。方法:BALB/c雌性小鼠48只,随机分为正常对照组,模型组,凉血活血胶囊高、中、低剂量组和雷公藤多苷组。采用银屑病皮损面积和疾病严重程度(psoriasis area and severity index,PASI)评分标准观察银屑病样小鼠模型皮损变化情况。光镜下观察皮损组织形态学变化,测量表皮层厚度。免疫组织化学法检测皮损中增殖细胞核抗原(proliferatingcell nuclear antigen,PCNA)反映表皮角质形成细胞增殖程度;检测CD3、CD11c、F4/80和Gr-1反映炎症浸润程度;检测CD31以反映血管增生情况。结果:模型组小鼠皮肤出现鳞屑红斑,皮损增厚;皮损组织表现为表皮棘层增厚,角化不全和微脓肿;真皮大量炎症细胞浸润,血管增生明显。与模型组比较,凉血活血胶囊组小鼠银屑病样皮损症状缓解,PASI分数降低,表皮角化不全减轻,角质形成增殖减少,T淋巴细胞、树突状细胞、巨噬细胞、中性粒细胞和单核细胞的浸润减弱,新生血管减少。结论:凉血活血胶囊可通过影响表皮细胞过度增殖、角化不全、炎症细胞浸润及血管增生而改善咪喹莫特诱导的小鼠银屑病样皮损变化。展开更多
Objective To investigate the effect of blood-cooling and promoting drugs (BCPD) on the dynamic changes of collagens and the expressions of interleukin-6 (IL-6)and transforming growth factor beta (TGF-β) in lung tissu...Objective To investigate the effect of blood-cooling and promoting drugs (BCPD) on the dynamic changes of collagens and the expressions of interleukin-6 (IL-6)and transforming growth factor beta (TGF-β) in lung tissue of rats with radiation-induced lung injury (RILI) to explore the effects and action mechanism of BCPD in preventing and treating RILI. Methods One hundred and sixty Wistar female rats were randomly divided into the radiation group, the treatment group, the blank control group and the drug control group. The rats in the first two groups received right hemithoracic fractionated radiation, and those in the treatment group were given BCPD. Rats in the other two groups were not irradiated and BCPD was given to rats in the drug control group. The rats were sacrificed in batches (8 of each group in every batch) at the 3rd, 5th, 8th, 12th and 26th week of the experimental period, and their lung was taken for observing the dynamic changes and distribution of collagen and the expressions of IL-6 and TGF-β with HE staining, picrosirius red staining and immunohistochemical staining respectively. Results The fibroblast proliferated obviously from the 3rd week after the first radiation in the radiation group, and the type Ⅰ collagen and the proportion of type Ⅰ and Ⅲ collagen were significantly elevated along the time going and the radiation dose increasing, became significantly higher than those in the treatment group at all the time points (P<0.01). In the radiation group the expression of IL-6 and TGF-β reached their peaks at the 8th and 12th week, respectively, and the levels was significantly lower in the treatment than that in the radiation group at any detecting time points (P<0.01). Conclusion BCPD applied in the early stage of radiation can suppress the inflammatory and fibrogenic cytokine expressions, inhibit the synthesis of collagens and adjust the proportion of type Ⅰ and Ⅲ collagen, so as to relieve the early-stage inflammatory reaction and the anaphase lung fibrosis in RILI rats.展开更多
文摘[目的]探究凉血活血汤配合常规西药治疗血热型银屑病并发冠心病的疗效,以及对患者皮肤屏障功能、血液流变学的影响。[方法]选取2019年11月至2021年12月血热型银屑病合并冠心病患者88例,随机分为两组,每组44例。对照组予以常规西药治疗,观察组予以凉血活血汤配合常规西药治疗。比较两组临床疗效、不良反应及治疗前后皮肤屏障功能、血液流变学指标、血清炎性因子白细胞介素-1(interleukin-1,IL-1)、IL-6、IL-8及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)及皮损面积和严重程度指数(psoriasis area and severity index,PASI)、Barthel指数(Barthel index,BI)。[结果]观察组治疗总有效率(88.64%)较对照组(70.45%)高(P<0.05),观察组不良反应发生率(13.64%)与对照组(9.09%)差异无统计学意义(P>0.05)。治疗2、4周后,观察组角质层含水量、皮脂含量均较对照组高,经表皮水分丢失量、全血高切、低切黏度、红细胞比容、血浆黏度及IL-1、IL-6、IL-8、TNF-α水平较对照组低(P<0.05)。治疗4、12周后,观察组PASI评分较对照组低,BI较对照组高(P<0.05)。[结论]凉血活血汤配合常规西药治疗银屑病合并冠心病患者,可有效减轻机体炎症反应,改善皮肤屏障功能及血液流变学指标,效果颇佳,且安全性高。
文摘目的:观察凉血活血胶囊对咪喹莫特诱导银屑病样小鼠模型中免疫异常的干预作用。方法:BALB/c雌性小鼠48只,随机分为正常对照组,模型组,凉血活血胶囊高、中、低剂量组和雷公藤多苷组。采用银屑病皮损面积和疾病严重程度(psoriasis area and severity index,PASI)评分标准观察银屑病样小鼠模型皮损变化情况。光镜下观察皮损组织形态学变化,测量表皮层厚度。免疫组织化学法检测皮损中增殖细胞核抗原(proliferatingcell nuclear antigen,PCNA)反映表皮角质形成细胞增殖程度;检测CD3、CD11c、F4/80和Gr-1反映炎症浸润程度;检测CD31以反映血管增生情况。结果:模型组小鼠皮肤出现鳞屑红斑,皮损增厚;皮损组织表现为表皮棘层增厚,角化不全和微脓肿;真皮大量炎症细胞浸润,血管增生明显。与模型组比较,凉血活血胶囊组小鼠银屑病样皮损症状缓解,PASI分数降低,表皮角化不全减轻,角质形成增殖减少,T淋巴细胞、树突状细胞、巨噬细胞、中性粒细胞和单核细胞的浸润减弱,新生血管减少。结论:凉血活血胶囊可通过影响表皮细胞过度增殖、角化不全、炎症细胞浸润及血管增生而改善咪喹莫特诱导的小鼠银屑病样皮损变化。
文摘Objective To investigate the effect of blood-cooling and promoting drugs (BCPD) on the dynamic changes of collagens and the expressions of interleukin-6 (IL-6)and transforming growth factor beta (TGF-β) in lung tissue of rats with radiation-induced lung injury (RILI) to explore the effects and action mechanism of BCPD in preventing and treating RILI. Methods One hundred and sixty Wistar female rats were randomly divided into the radiation group, the treatment group, the blank control group and the drug control group. The rats in the first two groups received right hemithoracic fractionated radiation, and those in the treatment group were given BCPD. Rats in the other two groups were not irradiated and BCPD was given to rats in the drug control group. The rats were sacrificed in batches (8 of each group in every batch) at the 3rd, 5th, 8th, 12th and 26th week of the experimental period, and their lung was taken for observing the dynamic changes and distribution of collagen and the expressions of IL-6 and TGF-β with HE staining, picrosirius red staining and immunohistochemical staining respectively. Results The fibroblast proliferated obviously from the 3rd week after the first radiation in the radiation group, and the type Ⅰ collagen and the proportion of type Ⅰ and Ⅲ collagen were significantly elevated along the time going and the radiation dose increasing, became significantly higher than those in the treatment group at all the time points (P<0.01). In the radiation group the expression of IL-6 and TGF-β reached their peaks at the 8th and 12th week, respectively, and the levels was significantly lower in the treatment than that in the radiation group at any detecting time points (P<0.01). Conclusion BCPD applied in the early stage of radiation can suppress the inflammatory and fibrogenic cytokine expressions, inhibit the synthesis of collagens and adjust the proportion of type Ⅰ and Ⅲ collagen, so as to relieve the early-stage inflammatory reaction and the anaphase lung fibrosis in RILI rats.
