宫颈鳞癌中子放疗前后细胞凋亡与PCNA的表达变化

目的:探讨252锎中子射线治疗宫颈癌的机制。方法:应用TUNEL法、免疫组化检测宫颈鳞癌放疗前后的细胞凋亡、PCNA表达变化。结果:20例患者宫颈癌细胞凋亡阳性率和凋亡指数(AI)分别由放疗前的35%和(4.22±4.14)%增加至放疗后的85%和(34.64±24.36)%,差异显著(P均小于0.01)。PCNA标记指数放疗后为(24.18±5.37)%,比放疗前的(48.67±5.62)%下降明显,两者之间具有显著性差异(P<0.01)。结论:252锎中子射线治疗宫颈癌的机制不仅在于诱导细胞凋亡,而且抑制了肿瘤细胞的增殖活性。

Effects of Res on proliferation and apoptosis of human cervical carcinoma cell lines C33A,SiHa and HeLa

Objective:To explore the effects of Res on the proliferation and apoptosis of human cervical cancer cell lines C33A,SiHa and HeLa.Methods:Inhibition rates by different concentrations of Res were calculated using MTT method.Apoptosis rates and cell cycles were measured and examined by flow cytometry(FCM).Morphological configuration of apoptotic cells were observed under the fluorescence microscope.Results:The growth of cancer cells was inhibited by Res of varied concentrations in a time-and dose-dependent manner(P<0.01).The cells showed characteristic apoptosis morphologic changes under fluorescence microscope.Res exerted no effects on cell cycles.Conclusion:Res inhibits the growth of cervical cell lines C33A,SiHa and HeLa by inducing cell apoptosis in a time-and dose-dependent manner.

VEGF-C诱导宫颈癌Hela细胞Bcl-2、cyclinD1的表达

目的研究VEGF-C对体外培养的宫颈癌Hela细胞增殖和凋亡的分子机制。方法应用重组人VEGF-C蛋白体外刺激宫颈癌Hela细胞,MTT法检测细胞增殖、流式细胞仪检测细胞周期和凋亡、Western Blotting检测增殖凋亡相关基因Bcl-2、cyclin D1蛋白水平的变化。结果 10、20、50 ng/μl VEGF-C处理后,细胞增殖指数分别为(1.00±0.03)、(1.25±0.05)、(1.55±0.08)、(2.13±0.08),呈剂量依赖性升高(P<0.05)。细胞周期S期比率呈剂量依赖性升高(P<0.05),分别为(30.91±0.09)%、(37.95±0.27)%、(45.05±0.40)%、(64.26±0.20)%;细胞凋亡率呈剂量依赖性降低(P<0.05),分别为(12.4±0.3)、(11.4±0.2)、(9.6±0.15)、(5.5±0.25)。Bcl-2、cyclin D1蛋白表达呈剂量依赖性升高。结论外源性VEGF-C通过细胞内信号的传递,诱导cyclin D1的表达,使肿瘤细胞S期加快,促进细胞周期的进程,来促进Hela细胞增殖;诱导Bcl-2的表达,抑制凋亡。