Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspens...Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspension polymerization respectively. The swelling kinetics of the gels was also studied. With these prepared PNIPA gels, the model protein lysozyme was renatured. Within 24h, PNIPA gel disks improved the yield of lysozyme activity by 49.3% from 3375.2U·mg^-1 to 5038.8U·mg^-1. With the addition of faster response PNIPA gel beads, the total lysozyme activity recovery was about 68.98% in 3h, as compared with 42.03% by simple batch dilution. The novel refolding system with PNIPA enables efficient refolding especially at high protein concentrations. Discussion about the mechanism revealed that when PNIPA gels were added into the refolding buffer, the hydrophobic interactions between denatured proteins and polymer gels could prevent the aggregation of refolding intermediates, thus enhanced the protein renaturation.展开更多
Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension...Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.展开更多
In this article, poly（2-hydroxyethylmethacrylate-co-acrylamide） hydrogels were synthesized by bulk free-radical copolymerization of 2-hydroxyethylmethacrylate （HEMA） and acrylamide （AAm） for soft contact lens（...In this article, poly（2-hydroxyethylmethacrylate-co-acrylamide） hydrogels were synthesized by bulk free-radical copolymerization of 2-hydroxyethylmethacrylate （HEMA） and acrylamide （AAm） for soft contact lens（SCL）-based ophthalmic drug delivery system. The copolymer was characterized with FT-IR and SEM, the swelling property of the hydrogels were studied by gravimetrical method, and chloramphenicol was used as a model drug to investigate drug release profile of the hydrogels. The results showed that poly（2-hydroxyethylmethacrylateco-acrylamide） hydrogels were transparent and useful SCL biomaterial, the water content increased as AAm content increase and pH decrease, and in the same way, hydrogel composition affected chloramphenicol release process too. Migration rate of chloramphenicol increased as the AAm content in the hydrogels increased in the first stage of diffusion process, whereas there was no significant difference thereafter.展开更多
By using strontium acetate and titanium n--butoxide [Ti(OBun)4] as starting agents, perovskite -- type double oxide SrTiO3 can be obtained by the sol--gel method. By means of infra-red spectra (IR), X--ray diffraction...By using strontium acetate and titanium n--butoxide [Ti(OBun)4] as starting agents, perovskite -- type double oxide SrTiO3 can be obtained by the sol--gel method. By means of infra-red spectra (IR), X--ray diffraction (XRD), differential thermal analysis (DTG), thermal gravimetry (TG) and scanning electron microscopy (SEM), basical factors, affecting the formation .of SrTiO3,have been discussed. IR results show that the acetic acid as a chelating ligand plays an important role in modifying the whole hydrolysis --condensation process. The addition of glycerol leads to obtaining clear and unique gels. The structure of SrTiO3 calcined at 800℃ is cubic.展开更多
The present study is an investigation of the properties of poly(vinyl alcohol),which would be a better contact lens mate- rial than conventional HEMA in some ways.A transparent PVA hydrogel was prepared from a PVA sol...The present study is an investigation of the properties of poly(vinyl alcohol),which would be a better contact lens mate- rial than conventional HEMA in some ways.A transparent PVA hydrogel was prepared from a PVA solution in a mixed solvent consisting of water and a water-miscible organic solvent,DMSO,by the freezing-thawing method.The water content,visible light transmittance,mechanical and swelling properties of the hydrogels were evaluated as a function of PVA concentration and number of freeze-thaw cycles.The results show that the properties of PVA hydrogels depend on the polymer concentration,the number of freeze-thaw cycles and the addition of the organic solvent.展开更多
A series of clay/poly（N-isopropylacrylamide-co-acrylamide） nanocomposite hydrogels （S-N-M gels） have been successfully prepared by in situ polymerization. The mechanical properties, swelling behavior of S-N-M gels...A series of clay/poly（N-isopropylacrylamide-co-acrylamide） nanocomposite hydrogels （S-N-M gels） have been successfully prepared by in situ polymerization. The mechanical properties, swelling behavior of S-N-M gels and the transparency changes during polymerization of S-N-M gels have been systematically investigated. Compared to traditional hydrogels, S-N-M gels show excellent tensile properties and their swelling ratio increases with increasing acrylamide （AAm） content. The results of stress relaxation indicate that the stress loss decreases with increasing AAm content. It was surprisingly found that the transparency during all S-N-M gel synthesis changes abruptly, and the changes become more abrupt with increasing N-isopropylacrylamide content. It was concluded that the fact may be related to the hydrophilicity of copolymers. The weaker the hydrophilicity of copolymer, the more apparent the transparency change during S-N-M gels polymerization. We believe the relationship between hydrophilicity of copolymer and transparency changes will help to design novel nanocomposite hydrogels.展开更多
To investigate the application of compound bioflocculant （CBF） in drinking water treatment at pilot plant, CBF and polymerized aluminium ferrum chloride （PAFC） coagulant were used to treat raw water taken from Lon...To investigate the application of compound bioflocculant （CBF） in drinking water treatment at pilot plant, CBF and polymerized aluminium ferrum chloride （PAFC） coagulant were used to treat raw water taken from Longhupao Reservoir in Heilongjiang Province for the removal of turbidity, COl）, UV254 and residual Al. Coagulation test shows that the coagulation enhanced by CBF and PAFC exhibits more effective performance than that enhanced by the individual of them, and the total combination dosage is lower than that of the individual. The residual Al from PAFC can be removed efficiently by CBF. The removal efficiency of turbidity reaches 76.6% by combining CBF of 2 mg/L and PAFC of 15 mg/L, COl） is decreased from 3.80 mg/L to 1.62 mg/ L, and the concentration of residual Al is only 0. 033 mg/L in the product water. It can be speculated that adsorption-bridging and sweep-coagulation processes are predominant in the flocculation process by the combination of CBF and PAFC.展开更多
Paclitaxel （PTX） is one of the most efficient anticancer drugs for the treatment of cancers through β-tubulin-binding. Our previous work indicated that a PTX-derivative hydrogelator Fmoc-Phe-Phe-Lys（paclitaxel）-...Paclitaxel （PTX） is one of the most efficient anticancer drugs for the treatment of cancers through β-tubulin-binding. Our previous work indicated that a PTX-derivative hydrogelator Fmoc-Phe-Phe-Lys（paclitaxel）-Tyr（H2PO3）-OH （1）could promote neuron branching but the underlying mechanism remains unclear. Using tubulin assembly-disassembly assay, in this work, we found that compound 1 obviously delayed more microtubule aggregation than PTX did. Under the catalysis of alkaline phosphatase, Fmoc-Phe-Phe-Lys（paclitaxel）- Tyr（H2PO3）-OH could self-assemble into nanofiber Fmoc-Phe-Phe-Lys（paclitaxel）-Tyr-OH with width comparable to the size of αβ-tubulin dimer. Therefore, we proposed in this work that nanofiber Fmoc-Phe-Phe-Lys（paclitaxel）-Tyr-OH not only inhibits the αβ-tubulin dimer binding to each other but also interferes with the plus end aggregation of microtubule. This work provides a new mechanism of the inhibition of microtubule formation by a PTX- derivative hydrogelator.展开更多
In order to remove the low turbidity present in surface water, a novel metal-polysilicate coagulant was used to treat the raw water taken from Tanjiang River in Guangdong Province. This study on the effects of Al/Fe ...In order to remove the low turbidity present in surface water, a novel metal-polysilicate coagulant was used to treat the raw water taken from Tanjiang River in Guangdong Province. This study on the effects of Al/Fe molar ratio on the performance of a complex compound formed by polysilicic acid, aluminium and ferric salt (PAFS) showed that PAFS with Al/Fe ratio of 10:3 seemed to have the best coagulation performance in removing turbidity and color. Experimental results showed that under the conditions of polymerization time of 15 d, sedimentation time of 12 min, and pH of 6?8, PAFS with Al/Fe molar ratio of 10:3 had the best coagulation efficiency and lowest residual Al concentration. The turbid- ity decreased from 23.8 NTU to 3.23 NTU and the residual Al concentration was only 0.165 mg/L in the product water. It could be speculated that colloidal impurities and particulate Al were removed by adsorption bridging and electrical neu- tralization of long chain inorganic polymer coagulants.展开更多
Delayed settling of the ash particles and poor drainage of water from the pond ash are the major problems faced during the hydraulic stowing of pond ash. In this study the effect of polymeric flocculant on settling of...Delayed settling of the ash particles and poor drainage of water from the pond ash are the major problems faced during the hydraulic stowing of pond ash. In this study the effect of polymeric flocculant on settling of the ash particles and drainage of water during pond ash stowing are investigated. In addition, the parameters, viz. drainage and absorption of water during pond ash stowing are quantified by stowing a mine goaf model with pond ash slurries of five different concentrations added with and without flocculant. The study revealed that addition of only 5 10 6 of Sodium Carboxymethyl Cellulose (Na-CMC) flocculant with the pond ash slurries during stowing offers best result in terms of quicker settling of the ash particles and enhanced water drainage from the hydraulically stowed pond ash. Besides, it resulted in drainage of more than 85% of the total water used in the initial 45 min of stowing. The improvement in drainage is caused due to coagulation and flocculation of the pond ash particles because of charge neutralization and particle-particle bridging. This study may provide a basis for estimating the drainage and absorption of water during the real pond ash stowing operation in underground mines.展开更多
This research investigated the water permeability coefficient of fly ash-based geopolymer concrete. The effect of sodium hydroxide （Na（OH）） concentrations and Si/AI ratios on water permeability and compressive str...This research investigated the water permeability coefficient of fly ash-based geopolymer concrete. The effect of sodium hydroxide （Na（OH）） concentrations and Si/AI ratios on water permeability and compressive strength of geopolymer concretes were studied. The geopolymer concrete were prepared from Mae Moh fly ash with sodium silicate （Na2SiO3） and sodium hydroxide （Na（OH）） solutions. In the first group, concentration of Na（OH） was varied at 8, 10, 12, and 14 molar and the Si/AI ratio was kept constant at 1.98. In the second group, a concentration of Na（OH） was kept constant at 14 molar and the Si/AI ratio was varied at 2.2, 2.4, 2.6, and 2.8. The hardened concretes were air-cured in laboratory. The compressive strength and water permeability were tested at the age of 28 and 60 days. The results showed that compressive strengths of geopolymer concrete significantly increased with the increase of a concentration of Na（OH） and Si/AI ratio. The water permeability coefficients increase with the decrease of compressive strength. In addition, the high reduction of water permeability coefficients with time was found in geopolymer concrete with lower Na（OH） concentration than that higher Na（OH） concentration.展开更多
The auto-gelling and drug release properties of the thermosensitive chitosan-β-glycerophosphate formulation were investigated. According to rheological study, gelation lag time of chitosan/β-glycerophosphate （GP） ...The auto-gelling and drug release properties of the thermosensitive chitosan-β-glycerophosphate formulation were investigated. According to rheological study, gelation lag time of chitosan/β-glycerophosphate （GP） solutions varied from 2 to 60min with different deacetylation degree of chitosan, pH, gelation temperature, and the particles in the sol. The gelation properties were also found to influence the release profilles of a hydrophilic drug, 5-fluorouracil （5-FU）. Morphological examination by scanning electron microphotography demonstrated that large ＂pores＂ occurred during the gel-forming process, which created hydrophilic environment and led to the rapid initial release of the drug （85% in f＇LrSt 8h）. Poly-3-hydroxybutyrate （PHB）, a biodegradable material, was applied here as scaffold to capture 5-FU into microparticles with high encapsulation efficiency by solvent-nonsolvent method. Combination of these microparticles into the chitosan-β-GP formulation could drop the rapid initial release from 85% down to 29% in the optimized PHB content （75%, by mass）. The release could sustain for about 10 months. Tiffs study provided an understanding of the potential of injectable implant using thermosensitive chitosan-β-GP formulation containing PHB based particles for the water soluble drugs that need the property of long-term delivery.展开更多
基金the National Natural Science Foundation of China (No. 20276065).
文摘Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspension polymerization respectively. The swelling kinetics of the gels was also studied. With these prepared PNIPA gels, the model protein lysozyme was renatured. Within 24h, PNIPA gel disks improved the yield of lysozyme activity by 49.3% from 3375.2U·mg^-1 to 5038.8U·mg^-1. With the addition of faster response PNIPA gel beads, the total lysozyme activity recovery was about 68.98% in 3h, as compared with 42.03% by simple batch dilution. The novel refolding system with PNIPA enables efficient refolding especially at high protein concentrations. Discussion about the mechanism revealed that when PNIPA gels were added into the refolding buffer, the hydrophobic interactions between denatured proteins and polymer gels could prevent the aggregation of refolding intermediates, thus enhanced the protein renaturation.
文摘Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.
基金Supported by the Natural Science Foundation of Guangdong Province （5300978） and Zhongkai University of Agriculture and Engineering （G2360221）.
文摘In this article, poly（2-hydroxyethylmethacrylate-co-acrylamide） hydrogels were synthesized by bulk free-radical copolymerization of 2-hydroxyethylmethacrylate （HEMA） and acrylamide （AAm） for soft contact lens（SCL）-based ophthalmic drug delivery system. The copolymer was characterized with FT-IR and SEM, the swelling property of the hydrogels were studied by gravimetrical method, and chloramphenicol was used as a model drug to investigate drug release profile of the hydrogels. The results showed that poly（2-hydroxyethylmethacrylateco-acrylamide） hydrogels were transparent and useful SCL biomaterial, the water content increased as AAm content increase and pH decrease, and in the same way, hydrogel composition affected chloramphenicol release process too. Migration rate of chloramphenicol increased as the AAm content in the hydrogels increased in the first stage of diffusion process, whereas there was no significant difference thereafter.
文摘By using strontium acetate and titanium n--butoxide [Ti(OBun)4] as starting agents, perovskite -- type double oxide SrTiO3 can be obtained by the sol--gel method. By means of infra-red spectra (IR), X--ray diffraction (XRD), differential thermal analysis (DTG), thermal gravimetry (TG) and scanning electron microscopy (SEM), basical factors, affecting the formation .of SrTiO3,have been discussed. IR results show that the acetic acid as a chelating ligand plays an important role in modifying the whole hydrolysis --condensation process. The addition of glycerol leads to obtaining clear and unique gels. The structure of SrTiO3 calcined at 800℃ is cubic.
基金Projects 50575106 supported by the National Natural Science Foundation of ChinaBG2007046 by the High Technology Project of Jiangsu Province
文摘The present study is an investigation of the properties of poly(vinyl alcohol),which would be a better contact lens mate- rial than conventional HEMA in some ways.A transparent PVA hydrogel was prepared from a PVA solution in a mixed solvent consisting of water and a water-miscible organic solvent,DMSO,by the freezing-thawing method.The water content,visible light transmittance,mechanical and swelling properties of the hydrogels were evaluated as a function of PVA concentration and number of freeze-thaw cycles.The results show that the properties of PVA hydrogels depend on the polymer concentration,the number of freeze-thaw cycles and the addition of the organic solvent.
基金the National Natural Science Foundation of China （Project No.50473002）,and the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China （No.704021）. the National High-tech 863 Project （2002AA302616）, and the Shanghai Nano Special Projects （0452nm006, 05nm05005）.
文摘A series of clay/poly（N-isopropylacrylamide-co-acrylamide） nanocomposite hydrogels （S-N-M gels） have been successfully prepared by in situ polymerization. The mechanical properties, swelling behavior of S-N-M gels and the transparency changes during polymerization of S-N-M gels have been systematically investigated. Compared to traditional hydrogels, S-N-M gels show excellent tensile properties and their swelling ratio increases with increasing acrylamide （AAm） content. The results of stress relaxation indicate that the stress loss decreases with increasing AAm content. It was surprisingly found that the transparency during all S-N-M gel synthesis changes abruptly, and the changes become more abrupt with increasing N-isopropylacrylamide content. It was concluded that the fact may be related to the hydrophilicity of copolymers. The weaker the hydrophilicity of copolymer, the more apparent the transparency change during S-N-M gels polymerization. We believe the relationship between hydrophilicity of copolymer and transparency changes will help to design novel nanocomposite hydrogels.
文摘To investigate the application of compound bioflocculant （CBF） in drinking water treatment at pilot plant, CBF and polymerized aluminium ferrum chloride （PAFC） coagulant were used to treat raw water taken from Longhupao Reservoir in Heilongjiang Province for the removal of turbidity, COl）, UV254 and residual Al. Coagulation test shows that the coagulation enhanced by CBF and PAFC exhibits more effective performance than that enhanced by the individual of them, and the total combination dosage is lower than that of the individual. The residual Al from PAFC can be removed efficiently by CBF. The removal efficiency of turbidity reaches 76.6% by combining CBF of 2 mg/L and PAFC of 15 mg/L, COl） is decreased from 3.80 mg/L to 1.62 mg/ L, and the concentration of residual Al is only 0. 033 mg/L in the product water. It can be speculated that adsorption-bridging and sweep-coagulation processes are predominant in the flocculation process by the combination of CBF and PAFC.
基金This work was supported by the Ministry of Science and Technology of China （No.2016YFA0400904） and the National Natural Science Foundation of China （No.U1532144 and No.21675145）.
文摘Paclitaxel （PTX） is one of the most efficient anticancer drugs for the treatment of cancers through β-tubulin-binding. Our previous work indicated that a PTX-derivative hydrogelator Fmoc-Phe-Phe-Lys（paclitaxel）-Tyr（H2PO3）-OH （1）could promote neuron branching but the underlying mechanism remains unclear. Using tubulin assembly-disassembly assay, in this work, we found that compound 1 obviously delayed more microtubule aggregation than PTX did. Under the catalysis of alkaline phosphatase, Fmoc-Phe-Phe-Lys（paclitaxel）- Tyr（H2PO3）-OH could self-assemble into nanofiber Fmoc-Phe-Phe-Lys（paclitaxel）-Tyr-OH with width comparable to the size of αβ-tubulin dimer. Therefore, we proposed in this work that nanofiber Fmoc-Phe-Phe-Lys（paclitaxel）-Tyr-OH not only inhibits the αβ-tubulin dimer binding to each other but also interferes with the plus end aggregation of microtubule. This work provides a new mechanism of the inhibition of microtubule formation by a PTX- derivative hydrogelator.
基金Project (No. E9825) supported by the Natural Science Foundation of Heilongjiang Province,China
文摘In order to remove the low turbidity present in surface water, a novel metal-polysilicate coagulant was used to treat the raw water taken from Tanjiang River in Guangdong Province. This study on the effects of Al/Fe molar ratio on the performance of a complex compound formed by polysilicic acid, aluminium and ferric salt (PAFS) showed that PAFS with Al/Fe ratio of 10:3 seemed to have the best coagulation performance in removing turbidity and color. Experimental results showed that under the conditions of polymerization time of 15 d, sedimentation time of 12 min, and pH of 6?8, PAFS with Al/Fe molar ratio of 10:3 had the best coagulation efficiency and lowest residual Al concentration. The turbid- ity decreased from 23.8 NTU to 3.23 NTU and the residual Al concentration was only 0.165 mg/L in the product water. It could be speculated that colloidal impurities and particulate Al were removed by adsorption bridging and electrical neu- tralization of long chain inorganic polymer coagulants.
文摘Delayed settling of the ash particles and poor drainage of water from the pond ash are the major problems faced during the hydraulic stowing of pond ash. In this study the effect of polymeric flocculant on settling of the ash particles and drainage of water during pond ash stowing are investigated. In addition, the parameters, viz. drainage and absorption of water during pond ash stowing are quantified by stowing a mine goaf model with pond ash slurries of five different concentrations added with and without flocculant. The study revealed that addition of only 5 10 6 of Sodium Carboxymethyl Cellulose (Na-CMC) flocculant with the pond ash slurries during stowing offers best result in terms of quicker settling of the ash particles and enhanced water drainage from the hydraulically stowed pond ash. Besides, it resulted in drainage of more than 85% of the total water used in the initial 45 min of stowing. The improvement in drainage is caused due to coagulation and flocculation of the pond ash particles because of charge neutralization and particle-particle bridging. This study may provide a basis for estimating the drainage and absorption of water during the real pond ash stowing operation in underground mines.
文摘This research investigated the water permeability coefficient of fly ash-based geopolymer concrete. The effect of sodium hydroxide （Na（OH）） concentrations and Si/AI ratios on water permeability and compressive strength of geopolymer concretes were studied. The geopolymer concrete were prepared from Mae Moh fly ash with sodium silicate （Na2SiO3） and sodium hydroxide （Na（OH）） solutions. In the first group, concentration of Na（OH） was varied at 8, 10, 12, and 14 molar and the Si/AI ratio was kept constant at 1.98. In the second group, a concentration of Na（OH） was kept constant at 14 molar and the Si/AI ratio was varied at 2.2, 2.4, 2.6, and 2.8. The hardened concretes were air-cured in laboratory. The compressive strength and water permeability were tested at the age of 28 and 60 days. The results showed that compressive strengths of geopolymer concrete significantly increased with the increase of a concentration of Na（OH） and Si/AI ratio. The water permeability coefficients increase with the decrease of compressive strength. In addition, the high reduction of water permeability coefficients with time was found in geopolymer concrete with lower Na（OH） concentration than that higher Na（OH） concentration.
基金Supported by the National Natural Science Foundation of China (No.20376038) and the Research Foundation of the Ministry ofEducation of China (No.2002003056).
文摘The auto-gelling and drug release properties of the thermosensitive chitosan-β-glycerophosphate formulation were investigated. According to rheological study, gelation lag time of chitosan/β-glycerophosphate （GP） solutions varied from 2 to 60min with different deacetylation degree of chitosan, pH, gelation temperature, and the particles in the sol. The gelation properties were also found to influence the release profilles of a hydrophilic drug, 5-fluorouracil （5-FU）. Morphological examination by scanning electron microphotography demonstrated that large ＂pores＂ occurred during the gel-forming process, which created hydrophilic environment and led to the rapid initial release of the drug （85% in f＇LrSt 8h）. Poly-3-hydroxybutyrate （PHB）, a biodegradable material, was applied here as scaffold to capture 5-FU into microparticles with high encapsulation efficiency by solvent-nonsolvent method. Combination of these microparticles into the chitosan-β-GP formulation could drop the rapid initial release from 85% down to 29% in the optimized PHB content （75%, by mass）. The release could sustain for about 10 months. Tiffs study provided an understanding of the potential of injectable implant using thermosensitive chitosan-β-GP formulation containing PHB based particles for the water soluble drugs that need the property of long-term delivery.
