Plasma 6-Keto-PGFla and thromboxane B2 (TXB2) were measured by RIA in patients (n=34) with peptic ulcer compared with no-peptic ulcer normal subjects (n=25). Gastric mucosa 6-Keto-PGFla and thromboxane B2 (TXB2) were ...Plasma 6-Keto-PGFla and thromboxane B2 (TXB2) were measured by RIA in patients (n=34) with peptic ulcer compared with no-peptic ulcer normal subjects (n=25). Gastric mucosa 6-Keto-PGFla and thromboxane B2 (TXB2) were measured in 30 patients with peptic ulcer and 20 normal mucosa by RIA. The TXB2/6-Keto-PGFla ratio value was significantly higher in patients with peptic ulcer than that in normal subjects in plasma (P<0.05) and in mucosa (P<0.01).展开更多
AIM:To explore the abnormal function of platelets and the role of angelica sinensis injection (ASI) in patients with ulcerative colitis (UC).METHODS:In 39 patients with active UC, 25 patients with remissive UC and 30 ...AIM:To explore the abnormal function of platelets and the role of angelica sinensis injection (ASI) in patients with ulcerative colitis (UC).METHODS:In 39 patients with active UC, 25 patients with remissive UC and 30 healthy people, α-granule membrane protein (GMP-140) and thromboxane B2 (TXB2) were detected by means of ELISA, 6-keto-PGF1α was detected by radioimmunoassay, platelet count (PC) and 1min platelet aggregation rate (1min PAR) were detected by blood automatic tester and platelet aggregation tester respectively,and von Willebrand factor related antigen (vWF:Ag) was detected by the means of monoclonal-ELISA. The 64 patients with UC were divided into two therapy groups. After routine treatment and angelica sinensis injection (ASI)+routine treatment respectively for 3 weeks, all these parameters were also detected.RESULTS:The PC, 1min PAR and levels of GMP-140,TXB2, and vWF:Ag in active UC were significanrly higher than those in remissive UC and normal controls (P<0.05-0.01).Meanwhile, 1 min PAR and levels of GMP-140, TXB2,and vWF:Ag in remissive UC were still significantly higher than those in normal controls (P<0.05). Furthermore, 6-keto-PGF1-α level in active and remissive UC was remarkably lower than that in normal control (P<0.05-0.01). These parameters except 6-keto-PGF1α were significantly improved after the treatment in ASI therapy group (P<0.05-0.01),whereas they all were little changed in routine therapy group (P>0.05).CONCLUSION:Platelets can be significantly activated in UC,which might be related with vascular endothelium injury and imbalance between TXB2 and 6-keto-PGF1α in blood.ASI can significantly inhibit platelet activation, relieve vascular endothelial cell injury, and improve microcirculation in UC.展开更多
文摘Plasma 6-Keto-PGFla and thromboxane B2 (TXB2) were measured by RIA in patients (n=34) with peptic ulcer compared with no-peptic ulcer normal subjects (n=25). Gastric mucosa 6-Keto-PGFla and thromboxane B2 (TXB2) were measured in 30 patients with peptic ulcer and 20 normal mucosa by RIA. The TXB2/6-Keto-PGFla ratio value was significantly higher in patients with peptic ulcer than that in normal subjects in plasma (P<0.05) and in mucosa (P<0.01).
基金Supported by Key Project in Scientific and Technological Researches of Hubei Province,No.2001AA308B
文摘AIM:To explore the abnormal function of platelets and the role of angelica sinensis injection (ASI) in patients with ulcerative colitis (UC).METHODS:In 39 patients with active UC, 25 patients with remissive UC and 30 healthy people, α-granule membrane protein (GMP-140) and thromboxane B2 (TXB2) were detected by means of ELISA, 6-keto-PGF1α was detected by radioimmunoassay, platelet count (PC) and 1min platelet aggregation rate (1min PAR) were detected by blood automatic tester and platelet aggregation tester respectively,and von Willebrand factor related antigen (vWF:Ag) was detected by the means of monoclonal-ELISA. The 64 patients with UC were divided into two therapy groups. After routine treatment and angelica sinensis injection (ASI)+routine treatment respectively for 3 weeks, all these parameters were also detected.RESULTS:The PC, 1min PAR and levels of GMP-140,TXB2, and vWF:Ag in active UC were significanrly higher than those in remissive UC and normal controls (P<0.05-0.01).Meanwhile, 1 min PAR and levels of GMP-140, TXB2,and vWF:Ag in remissive UC were still significantly higher than those in normal controls (P<0.05). Furthermore, 6-keto-PGF1-α level in active and remissive UC was remarkably lower than that in normal control (P<0.05-0.01). These parameters except 6-keto-PGF1α were significantly improved after the treatment in ASI therapy group (P<0.05-0.01),whereas they all were little changed in routine therapy group (P>0.05).CONCLUSION:Platelets can be significantly activated in UC,which might be related with vascular endothelium injury and imbalance between TXB2 and 6-keto-PGF1α in blood.ASI can significantly inhibit platelet activation, relieve vascular endothelial cell injury, and improve microcirculation in UC.
