目的观察凝血因子VII(coagulationf actor VII,FVII)R353Q基因多态性在华南汉族健康者人群中的分布。方法提取华南汉族60名正常健康者基因组DNA,应用多聚酶链反应(PCR)技术和限制性内切酶片段长度多态性技术检测上述人群的FVII R353Q...目的观察凝血因子VII(coagulationf actor VII,FVII)R353Q基因多态性在华南汉族健康者人群中的分布。方法提取华南汉族60名正常健康者基因组DNA,应用多聚酶链反应(PCR)技术和限制性内切酶片段长度多态性技术检测上述人群的FVII R353Q基因型。结果华南汉族人群FVII基因R353Q具3种基因型(RR,RQ,QQ),FVII等位基因R、Q基因频率在人群中分别为90.9%,9.1%。基因型频率符合Hardy-Weinberg平衡定律。结论华南汉族健康人群凝血因子VII基因R353Q多态具3种基因型(RR,RQ,QQ),R等位基因携带频率明显高于Q等位基因。展开更多
Low factor V and VII levels are bad prognostic indicators in fulminant hepatic failure (FHF). The prognostic importance of admission versus follow up levels of these factors in patients with acute hepatitis and coagul...Low factor V and VII levels are bad prognostic indicators in fulminant hepatic failure (FHF). The prognostic importance of admission versus follow up levels of these factors in patients with acute hepatitis and coagulopathy without encephalopathy has not been evaluated. Clinical and laboratory data from 68 consecutive patients with acute hepatitis and coagulopathy but without encephalopathy, during a 6-year period, was retrospectively evaluated. Sixty patients (88%) demonstrated improvement in liver function and coagulation (‘survivors’), while 8 patients (12%) died or underwent OLT (‘non-survivors’). Survivors had higher admission (P < 0.005) and follow up factor VII levels (P < 0.005) than non-survivors. Follow up factor V levels were higher in survivors (P < 0.02), while admission factor V level was not different between groups (P=NS). Multivariate logistic regression analysis demonstrated that admission factor VII levels predicted outcome (P < 0.006). Area under the ROC curve of factor VII was larger than that of factor V (0.885 and 0.715, respectively, P < 0.02). After 3 days of hospitalization, factor V levels, but not factor VII, independently predicted outcome (P < 0.04). In patients with hepatitis and coagulopathy without encephalopathy at presentation, admission factor VII level may serve as a reliable prognostic marker. Subsequently, during hospitalization, changes in factor V are better outcome indicators.展开更多
文摘目的观察凝血因子VII(coagulationf actor VII,FVII)R353Q基因多态性在华南汉族健康者人群中的分布。方法提取华南汉族60名正常健康者基因组DNA,应用多聚酶链反应(PCR)技术和限制性内切酶片段长度多态性技术检测上述人群的FVII R353Q基因型。结果华南汉族人群FVII基因R353Q具3种基因型(RR,RQ,QQ),FVII等位基因R、Q基因频率在人群中分别为90.9%,9.1%。基因型频率符合Hardy-Weinberg平衡定律。结论华南汉族健康人群凝血因子VII基因R353Q多态具3种基因型(RR,RQ,QQ),R等位基因携带频率明显高于Q等位基因。
文摘Low factor V and VII levels are bad prognostic indicators in fulminant hepatic failure (FHF). The prognostic importance of admission versus follow up levels of these factors in patients with acute hepatitis and coagulopathy without encephalopathy has not been evaluated. Clinical and laboratory data from 68 consecutive patients with acute hepatitis and coagulopathy but without encephalopathy, during a 6-year period, was retrospectively evaluated. Sixty patients (88%) demonstrated improvement in liver function and coagulation (‘survivors’), while 8 patients (12%) died or underwent OLT (‘non-survivors’). Survivors had higher admission (P < 0.005) and follow up factor VII levels (P < 0.005) than non-survivors. Follow up factor V levels were higher in survivors (P < 0.02), while admission factor V level was not different between groups (P=NS). Multivariate logistic regression analysis demonstrated that admission factor VII levels predicted outcome (P < 0.006). Area under the ROC curve of factor VII was larger than that of factor V (0.885 and 0.715, respectively, P < 0.02). After 3 days of hospitalization, factor V levels, but not factor VII, independently predicted outcome (P < 0.04). In patients with hepatitis and coagulopathy without encephalopathy at presentation, admission factor VII level may serve as a reliable prognostic marker. Subsequently, during hospitalization, changes in factor V are better outcome indicators.
