The selection and application of salt tolerance varieties in peanut is impor- tant for the exploitation of saline soil. In this study, 41 peanut varieties were select- ed for the evaluation of salt tolerance in both g...The selection and application of salt tolerance varieties in peanut is impor- tant for the exploitation of saline soil. In this study, 41 peanut varieties were select- ed for the evaluation of salt tolerance in both germination and young seedling stage. The results showed the germination and growth of peanut were significantly inhibited by salt stress, which were enhanced by salt concentration increasing. The 0.5% NaCI was selected as the most suitable concentration for further identification, and the characters in germination stage (including the relative germination potential, relative germination rate and germination index) and young seedling stage (including relative main root length, seedling height, fresh weight, dry weight, et aL) were measured. Cluster analysis showed the varieties could be divided into different groups, and 9 and 3 salt-tolerant varieties were identified in the two stages, respec- tively, including two varieties performed well in both two stages, which could be useful for the breeding of new salt tolerance varieties and the study of salt toler- ance mechanism.展开更多
Objective: To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluores- cein isothiocyanate (FITC) after trauma-hemorrhage in mice. Methods: Inbred BALB/c mice (6-...Objective: To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluores- cein isothiocyanate (FITC) after trauma-hemorrhage in mice. Methods: Inbred BALB/c mice (6-8 weeks old, male) were epicutaneously sensitized with FITC 12 hours, 1 day, 2 days, 4 days and 7 days after closed bilateral femur fractures combined with hemorrhage. And 5 days after sensitization, DTH was evaluated by ear swelling after a challenge of FITC. Draining lymph node cells were examined for the percentages of FITC-positive cells, cluster of differentiation (CD)11c positive cells and major histocom- patibility complex II (MHC II)-positive cells by means of flow cytometry. In vitro proliferative responses of syngeneic lymphocytes and in vivo passive transfer of DTH to naive recipients induced by isolated DCs from the draining lymph nodes were determined. Results: The time of DTH to FITC decreased more significantly in the mice with trauma-hemorrhage (12 hours to 4 days) than in the mice with sham injury. After sensitization, the relative percentages of FITC^+ cells, FITC^+/ CD 11c^+ cells and FITC^+/CD 11c^+/MHC II^+ cells from the draining lymph nodes were all significantly reduced following injury. And the capacity of DCs from the draining lymph nodes in stimulating proliferative responses of lymphocytes and transferring DTH to naive recipients were also inhibited after injury. Conclusions: Trauma-hemorrhage induces repressive DTH in mice, which may be attributed, at least partially, to the reduced trafficking of DCs into the draining lymph nodes and insufficient maturation during DC migration.展开更多
We present a low cost radio-frequency (RF) generator suitable for experiments with cold atoms. The RF source achieves a sub-hertz frequency with tunable resolution from 0 MHz to 400 MHz and a maximum output power of...We present a low cost radio-frequency (RF) generator suitable for experiments with cold atoms. The RF source achieves a sub-hertz frequency with tunable resolution from 0 MHz to 400 MHz and a maximum output power of 33 dBm. Based on a direct digital synthesizer (DDS) chip, we implement a ramping capability for frequency, ampli- tude and phase. The system can also operate as an arbitrary waveform generator. By measuring the stability in a dura- tion of 600 s, we fred the presented device performs comparably as Agilent33522A in terms of short-term stability. Due to its excellent performance, the RF generator has been already applied to cold atom trapping experiments.展开更多
文摘The selection and application of salt tolerance varieties in peanut is impor- tant for the exploitation of saline soil. In this study, 41 peanut varieties were select- ed for the evaluation of salt tolerance in both germination and young seedling stage. The results showed the germination and growth of peanut were significantly inhibited by salt stress, which were enhanced by salt concentration increasing. The 0.5% NaCI was selected as the most suitable concentration for further identification, and the characters in germination stage (including the relative germination potential, relative germination rate and germination index) and young seedling stage (including relative main root length, seedling height, fresh weight, dry weight, et aL) were measured. Cluster analysis showed the varieties could be divided into different groups, and 9 and 3 salt-tolerant varieties were identified in the two stages, respec- tively, including two varieties performed well in both two stages, which could be useful for the breeding of new salt tolerance varieties and the study of salt toler- ance mechanism.
基金This study was supported by the National Basic Research Program of China (No. 2005CB522602), Natural Science Foundation of China (No.30772253) and Project of State Key Laboratory of Trauma, Burns, and Combined Injury (No. SKLZZ200802).
文摘Objective: To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluores- cein isothiocyanate (FITC) after trauma-hemorrhage in mice. Methods: Inbred BALB/c mice (6-8 weeks old, male) were epicutaneously sensitized with FITC 12 hours, 1 day, 2 days, 4 days and 7 days after closed bilateral femur fractures combined with hemorrhage. And 5 days after sensitization, DTH was evaluated by ear swelling after a challenge of FITC. Draining lymph node cells were examined for the percentages of FITC-positive cells, cluster of differentiation (CD)11c positive cells and major histocom- patibility complex II (MHC II)-positive cells by means of flow cytometry. In vitro proliferative responses of syngeneic lymphocytes and in vivo passive transfer of DTH to naive recipients induced by isolated DCs from the draining lymph nodes were determined. Results: The time of DTH to FITC decreased more significantly in the mice with trauma-hemorrhage (12 hours to 4 days) than in the mice with sham injury. After sensitization, the relative percentages of FITC^+ cells, FITC^+/ CD 11c^+ cells and FITC^+/CD 11c^+/MHC II^+ cells from the draining lymph nodes were all significantly reduced following injury. And the capacity of DCs from the draining lymph nodes in stimulating proliferative responses of lymphocytes and transferring DTH to naive recipients were also inhibited after injury. Conclusions: Trauma-hemorrhage induces repressive DTH in mice, which may be attributed, at least partially, to the reduced trafficking of DCs into the draining lymph nodes and insufficient maturation during DC migration.
基金This work has been supported by the National Natural Science Foundation of China(No.51275523)the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20134307110009)
文摘We present a low cost radio-frequency (RF) generator suitable for experiments with cold atoms. The RF source achieves a sub-hertz frequency with tunable resolution from 0 MHz to 400 MHz and a maximum output power of 33 dBm. Based on a direct digital synthesizer (DDS) chip, we implement a ramping capability for frequency, ampli- tude and phase. The system can also operate as an arbitrary waveform generator. By measuring the stability in a dura- tion of 600 s, we fred the presented device performs comparably as Agilent33522A in terms of short-term stability. Due to its excellent performance, the RF generator has been already applied to cold atom trapping experiments.