期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
用Raman光谱法研究出血蛋白酶的二级结构
1
作者 郑思定 舒莲美 +1 位作者 冉永禄 杜祖健 《复旦学报(自然科学版)》 CAS CSCD 北大核心 1989年第3期278-283,共6页
用喇曼光谱方法测定了蛇毒出血蛋白酶的二级结构。通过分析酰胺Ⅰ和酰胺Ⅲ的喇曼特征频率,可以判定LHTa和LHTb是α-螺旋、β-回折和无规卷曲的结构,与用Lippert方法进行计算所得的结果相同。
关键词 出血蛋白酶 蛋白酶 喇曼光谱 结构
原文传递
大鼠实验性脑出血后基质金属蛋白酶2和9的表达 被引量:15
2
作者 田力 刘海峰 滕伟禹 《中国动脉硬化杂志》 CAS CSCD 2006年第3期221-223,共3页
目的探讨大鼠脑出血后基质金属蛋白酶2和基质金属蛋白酶9的表达及其意义。方法采用未抗凝新鲜自体股动脉血注入大鼠尾状核建立脑出血动物模型。用酶谱分析法测定脑出血后6h、12h、24h、3天、6天、10天和15天时基质金属蛋白酶2和基质金... 目的探讨大鼠脑出血后基质金属蛋白酶2和基质金属蛋白酶9的表达及其意义。方法采用未抗凝新鲜自体股动脉血注入大鼠尾状核建立脑出血动物模型。用酶谱分析法测定脑出血后6h、12h、24h、3天、6天、10天和15天时基质金属蛋白酶2和基质金属蛋白酶9的表达。结果基质金属蛋白酶2在脑出血后24h开始表达(P<0.05),第6天时达高峰(P<0.01),到第10天仍维持高水平表达(P<0.05);而基质金属蛋白酶9在脑出血后6h开始表达(P<0.05),24h^3天最明显(P<0.01),此后活性逐渐降低,第10天时的活性仍高于对照组(P>0.05),15天时不再表达。结论脑出血后6h^3天出血灶周围脑组织中基质金属蛋白酶9的活性增高,可能与急性期脑水肿的形成有关,而基质金属蛋白酶2的迟发表达可能参与了脑组织的修复过程。 展开更多
关键词 神经病学 出血后基质金属蛋白酶2和9的表达 酶谱分析法 出血 基质金属蛋白酶2 基质金属蛋白酶9 脑水肿
下载PDF
Purification and Characterization of Jerdonitin, a Non-hemorrhagic Metalloproteinase from Trimeresurus jerdonii Venom 被引量:1
3
作者 陈润强 金扬 +5 位作者 吴健波 钟树荣 朱绍文 吕秋敏 王婉瑜 熊郁良 《Zoological Research》 CAS CSCD 北大核心 2005年第6期616-621,共6页
Previously, we have purified Jerdonitin from Trimeresurusjerdonii venom. Compared with other P-Ⅱ class snake venom metalloproteinases (SVMPs), Jerdonitin has a primary structure comprising metalloproteinase and dis... Previously, we have purified Jerdonitin from Trimeresurusjerdonii venom. Compared with other P-Ⅱ class snake venom metalloproteinases (SVMPs), Jerdonitin has a primary structure comprising metalloproteinase and disintegrin domains. However, no hemorrhagic and fibrinogenolytic activities were detected for Jerdonitin. We thought that organic buffer of high performance liquid charamatography (HPLC) might affect its enzymatic activity. In this study, we purified Jerdonitin by another procedure excluding the HPLC. It was homogenous as judged by SDS-PAGE and had an apparent molecular weight of 36 kDa under non-reducing conditions and 38 kDa under reducing conditions, respectively. Like other typical metalloproteinases, Jerdonitin preferentially degraded alpha-chain of human fibrinogen and this fibrinogenolytic activity was completely inhibited by EDTA, but not by PMSF. It was interesting that Jerdonitin did not induce hemorrhage after intradermal injection in mice. 展开更多
关键词 Trimeresurus jerdonii SVMP HEMORRHAGE Fibrinogenolytic activity
下载PDF
Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats 被引量:1
4
作者 Yun ZHANG Zhong-yan LIANG +4 位作者 Shao-yang ZHANG Fang-fang HUANG Wei WU Yuan GAO Zuo-bing CHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第11期871-878,共8页
Objective: To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats. Methods: Studies were performed on an in vivo model of spontaneously breathing rat... Objective: To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats. Methods: Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected. Results: Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor κB (NF-κB), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats. Conclusion: Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-κB. 展开更多
关键词 Traumatic/hemorrhagic shock (T/HS) Mitogen-activated protein kinase (MAPK) Nuclear factor κB (NF-κB) ALBUMIN APOPTOSIS
下载PDF
A POTENTIAL MECHANISM OF BREAKTHROUGH BLEEDING ASSOCIATED WITH PROGESTIN:INVOLVEMENT IN ALTERATION OF ENDOMETRIAL ENDOTHELIAL CELLS 被引量:1
5
作者 Gui-hua Sha Shou-qing Lin 《Chinese Medical Sciences Journal》 CAS CSCD 2008年第1期32-37,共6页
Objective To explore the potential mechanism of breakthrough bleeding associated with progestin with in vitro methods.Methods The isolation and culture of human endometrial endothelial cells(HEECs)was performed with t... Objective To explore the potential mechanism of breakthrough bleeding associated with progestin with in vitro methods.Methods The isolation and culture of human endometrial endothelial cells(HEECs)was performed with the method established in our laboratory.The content and activity of urokinase-type plasminogen activator(uPA)and the content of plasminogen activator inhibitor-1(PAI-1)in cell supernatants after incubated with different concentrations of progesterone(0-5 μmol/L)and 17β-estradiol(0,0.1,or 1 nmol/L)were measured by method of ELISA.Apoptosis rate of HEECs was measured by flow cytometry.Viable cell count was measured by MTT.Results The increased level of progesterone(0.5-5 μmol/L)combined with 17β-estradiol elevated content and activity of uPA while the production of PAI-1 remained unchanged.The apoptosis of HEECs was inhibited along with the increment of total viable cell counts at higher concentrations of progesterone with 17β-estradiol.Conclusion The inhibition of apoptosis and increased content and activity of uPA may contribute to the occurrence of irregular bleeding associated with progestin use to some extent. 展开更多
关键词 PROGESTERONE 17Β-ESTRADIOL breakthrough bleeding apoptosis urokinase-type plasminogen activator
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部