[Objective] The study aimed to investigate the changes of water status and different responses of osmoregulants during air-drought stress,to better understand mechanisms of drought resistance in Jatropha Curcas L. [Me...[Objective] The study aimed to investigate the changes of water status and different responses of osmoregulants during air-drought stress,to better understand mechanisms of drought resistance in Jatropha Curcas L. [Methods] The 12-day-old J. curcas seedlings were held in a climate chamber at 25/20 ℃（day/night）,16 hours illumination,and 75% of relative humidity for air-drought treatment,and the changes of water potential,osmotic potential and the content of soluble sugar,proline,betaine were measured. [Results] Water potential and osmotic potential in leaves of J. curcas seedlings dropped significantly,pressure potential lost during air-drought stress,and the contents of osmoregulants soluble sugar,proline and betaine rose significantly to different extent in the leaves and stems. [Conclusion] Osmoregulants in the leaves and stems respond differently to air-drought stress,and in general leaves are much more responsive to the drought than stems of J. curcas seedlings.展开更多
The permselectivity of H2/O2, H2/N2, H2/CO, and H2/CH4 mixtures passing a graphdiyne membrane is studied by molecular dynamics simulations. At pressure range of 0.047-4.5 GPa, H2 can pass the graphdiyen membrane quick...The permselectivity of H2/O2, H2/N2, H2/CO, and H2/CH4 mixtures passing a graphdiyne membrane is studied by molecular dynamics simulations. At pressure range of 0.047-4.5 GPa, H2 can pass the graphdiyen membrane quickly, while all the O2, N2, CO, and CH4 molecules are blocked. At pressure of 47 kPa, the hydrogen flow is 7 mol/m^2s. With increase of pressure, the hydrogen flow goes up, and reaches maximum of 6×10^5 mol/m^2s at 1.5 GPa. Compared to other known membranes, graphdiyne can be used for means of hydrogen purification with the best balance of high selectivity and high permeance.展开更多
Reinforcement inside the concrete is protected from corrosion and its damages until several years after the construction. After corrosion initiation, the cross section of reinforcement begins to reduce and often load ...Reinforcement inside the concrete is protected from corrosion and its damages until several years after the construction. After corrosion initiation, the cross section of reinforcement begins to reduce and often load bearing of the reinforced concrete structure will be reduced significantly. Corrosion of reinforcements in concrete in polluted and contaminated areas can occur in two ways: chloride and carbonation. In this work, meta-heuristic approach of charged system search(CSS) is used to calculate corrosion occurrence probability due to chloride ions penetration. The model efficiency is verified by comparing the available examples in technical literature and results of Monte Carlo analysis. According to the analyses performed, using different probabilistic distributions regardless of probabilistic moments based on real distribution leads to diverse results. In addition, influence of each effective parameter in corrosion occurrence varies by changing other parameters.展开更多
In this study, orange G dye was efficiently removed from aqueous solution by ultraflltration （UF） membrane separation enhanced with activated carbon adsorption. The powdered activated carbon （PAC） was deposited on...In this study, orange G dye was efficiently removed from aqueous solution by ultraflltration （UF） membrane separation enhanced with activated carbon adsorption. The powdered activated carbon （PAC） was deposited onto the UF membrane surface, forming an intact filter cake. The enhanced UF process simultaneously exploited the high water permeation flux of porous membrane and the high adsorption ability of PAC toward dye molecules. The influencing factors on the dye removal were investigated. The results indicated that with sufficient PAC incorporation, the formation of intact PAC filtration cake led to nearly complete rejection for dye solution under opti-mized dye concentration and operation pressure, without large sacnticlng the permeation tlux ot the filtration process. Typically, the dye rejection ratio increased from 43.6% for single UF without adsorption to nearly 100% for the enhanced UF process, achieving long time continuous treatment with water permeation flux of 47 L·m^-2·h^-1. The present study demonstrated that adsorption enhanced UF may be a feasible method for the dye wastewater treatment.展开更多
[Objective] The aim was to investigate the dimer formation between the movement proteins（MP）in barely yellow dwarf virus by using the technology of bimolecular fluorescence complementation technology and to further ...[Objective] The aim was to investigate the dimer formation between the movement proteins（MP）in barely yellow dwarf virus by using the technology of bimolecular fluorescence complementation technology and to further study the relationship between MP homodimerization and viral movement.[Method] The DNA sequence of bimolecular fluorescent complementary vector containing cloning multiple cloning sites,35S promoter and terminator was cloned into the expression vector pCAMBIA1300,which replicates at a higher copy number in E.coli.Then,the BYDV-MP gene fragment was amplified in the presence of the whole BYDV-PAV cDNA sequence as template and the primers designed according to the BYDV-MP gene sequence from GenBank,cloned into the modified bimolecular fluorescent complementary vectors pCAMBIA1300-NE and pCAMBIA1300-CE.The resulting vectors were transformed into Agrobacterium by electroporation method and infiltrated into the tobacco leaf.Protein interactions were observed under fluorescence microscope.[Result] Yellow fluorescence could be viewed in the leaves co-infiltrated with Agrobacterium carrying pCAMBIA1300NE-MP and pCAMBIA1300CE-MP at 2-5 d post-infiltration,while yellow fluorescence could not be observed in negative control groups.[Conclusion] BYDV-MP formed homodimers in plant cells.The results can provide theoretical basis for further in-depth research about the movement process and mechanism of BYDV.展开更多
This paper presented an investigation of particle collision and penetration using the discrete element method to understand the motion of particles and improve theoretical treatment in the sieving process. The process...This paper presented an investigation of particle collision and penetration using the discrete element method to understand the motion of particles and improve theoretical treatment in the sieving process. The process progressively was divided into looseness, stratification, collision, and penetration. Particle penetration has a direct effect on the screening performance. The penetration probability was defined, and the mathematical relationships between particle penetration and vibration parameters were established using the least squares method. To obtain the ideal penetration probability for materials the amplitude and frequency should preferably be near 3.0 mm and 25 Hz, respectively. The vibration direction angle has only a slight effect on penetration. The stage of the screening process from 0.1 to 0.7 s is the primary region for collision and penetration. This paper focused on the sieving process to more fully understand how particle collision and penetration influence the screening efficiency.展开更多
基金Supported by Special Key R&D Fund from Yunnan Provincial Department of Education (ZD2010004)~~
文摘[Objective] The study aimed to investigate the changes of water status and different responses of osmoregulants during air-drought stress,to better understand mechanisms of drought resistance in Jatropha Curcas L. [Methods] The 12-day-old J. curcas seedlings were held in a climate chamber at 25/20 ℃（day/night）,16 hours illumination,and 75% of relative humidity for air-drought treatment,and the changes of water potential,osmotic potential and the content of soluble sugar,proline,betaine were measured. [Results] Water potential and osmotic potential in leaves of J. curcas seedlings dropped significantly,pressure potential lost during air-drought stress,and the contents of osmoregulants soluble sugar,proline and betaine rose significantly to different extent in the leaves and stems. [Conclusion] Osmoregulants in the leaves and stems respond differently to air-drought stress,and in general leaves are much more responsive to the drought than stems of J. curcas seedlings.
基金V. ACKNOWLEDGMENTS This work was supported by the National Natu- ral Science Foundation of China （No.20603032 and 20733004）, the National Key Basic Research Program （No.2011CB921400）, the Foundation of National Excellent Doctoral Dissertation of China （No.200736）, the Fundamental Research Funds for the Central Universities （No.WK2340000006 and No.WK2060140005）, and the Shanghai Supercomputer Center, the USTC-HP HPC Project, and the SCCAS.
文摘The permselectivity of H2/O2, H2/N2, H2/CO, and H2/CH4 mixtures passing a graphdiyne membrane is studied by molecular dynamics simulations. At pressure range of 0.047-4.5 GPa, H2 can pass the graphdiyen membrane quickly, while all the O2, N2, CO, and CH4 molecules are blocked. At pressure of 47 kPa, the hydrogen flow is 7 mol/m^2s. With increase of pressure, the hydrogen flow goes up, and reaches maximum of 6×10^5 mol/m^2s at 1.5 GPa. Compared to other known membranes, graphdiyne can be used for means of hydrogen purification with the best balance of high selectivity and high permeance.
文摘Reinforcement inside the concrete is protected from corrosion and its damages until several years after the construction. After corrosion initiation, the cross section of reinforcement begins to reduce and often load bearing of the reinforced concrete structure will be reduced significantly. Corrosion of reinforcements in concrete in polluted and contaminated areas can occur in two ways: chloride and carbonation. In this work, meta-heuristic approach of charged system search(CSS) is used to calculate corrosion occurrence probability due to chloride ions penetration. The model efficiency is verified by comparing the available examples in technical literature and results of Monte Carlo analysis. According to the analyses performed, using different probabilistic distributions regardless of probabilistic moments based on real distribution leads to diverse results. In addition, influence of each effective parameter in corrosion occurrence varies by changing other parameters.
基金Supported by Drug Separation and Purification Project in Programme for Development of Novel Drug (2009ZX09301-008)the Program of Introducing Talents of Discipline to Universities (B06006)State Key Laboratory of Precision Measuring Technology and Instruments (Tianjin University)
文摘In this study, orange G dye was efficiently removed from aqueous solution by ultraflltration （UF） membrane separation enhanced with activated carbon adsorption. The powdered activated carbon （PAC） was deposited onto the UF membrane surface, forming an intact filter cake. The enhanced UF process simultaneously exploited the high water permeation flux of porous membrane and the high adsorption ability of PAC toward dye molecules. The influencing factors on the dye removal were investigated. The results indicated that with sufficient PAC incorporation, the formation of intact PAC filtration cake led to nearly complete rejection for dye solution under opti-mized dye concentration and operation pressure, without large sacnticlng the permeation tlux ot the filtration process. Typically, the dye rejection ratio increased from 43.6% for single UF without adsorption to nearly 100% for the enhanced UF process, achieving long time continuous treatment with water permeation flux of 47 L·m^-2·h^-1. The present study demonstrated that adsorption enhanced UF may be a feasible method for the dye wastewater treatment.
基金Supported by National Natural Science Foundation of China(30870109)~~
文摘[Objective] The aim was to investigate the dimer formation between the movement proteins（MP）in barely yellow dwarf virus by using the technology of bimolecular fluorescence complementation technology and to further study the relationship between MP homodimerization and viral movement.[Method] The DNA sequence of bimolecular fluorescent complementary vector containing cloning multiple cloning sites,35S promoter and terminator was cloned into the expression vector pCAMBIA1300,which replicates at a higher copy number in E.coli.Then,the BYDV-MP gene fragment was amplified in the presence of the whole BYDV-PAV cDNA sequence as template and the primers designed according to the BYDV-MP gene sequence from GenBank,cloned into the modified bimolecular fluorescent complementary vectors pCAMBIA1300-NE and pCAMBIA1300-CE.The resulting vectors were transformed into Agrobacterium by electroporation method and infiltrated into the tobacco leaf.Protein interactions were observed under fluorescence microscope.[Result] Yellow fluorescence could be viewed in the leaves co-infiltrated with Agrobacterium carrying pCAMBIA1300NE-MP and pCAMBIA1300CE-MP at 2-5 d post-infiltration,while yellow fluorescence could not be observed in negative control groups.[Conclusion] BYDV-MP formed homodimers in plant cells.The results can provide theoretical basis for further in-depth research about the movement process and mechanism of BYDV.
文摘This paper presented an investigation of particle collision and penetration using the discrete element method to understand the motion of particles and improve theoretical treatment in the sieving process. The process progressively was divided into looseness, stratification, collision, and penetration. Particle penetration has a direct effect on the screening performance. The penetration probability was defined, and the mathematical relationships between particle penetration and vibration parameters were established using the least squares method. To obtain the ideal penetration probability for materials the amplitude and frequency should preferably be near 3.0 mm and 25 Hz, respectively. The vibration direction angle has only a slight effect on penetration. The stage of the screening process from 0.1 to 0.7 s is the primary region for collision and penetration. This paper focused on the sieving process to more fully understand how particle collision and penetration influence the screening efficiency.
