The molecular karyotypes of strain NJ7 and NJS of Debaryomyces hansenii were obscened byusing pulsed-field gel electrophoresis (PFGE) which employs contour-clamped homogeneous electric field (CHEF).Both strains were w...The molecular karyotypes of strain NJ7 and NJS of Debaryomyces hansenii were obscened byusing pulsed-field gel electrophoresis (PFGE) which employs contour-clamped homogeneous electric field (CHEF).Both strains were with & chromosomal DNAs. The eshmated chromosomal DNA sizes of strain NJ7 were 0.47Mb,0.89Mb, 1.05Mb, 1.36Mb, 2.20Mb, 2.20Mb, 2.80Mb, 3.05Mb, and srain NJS were 0.47Mb, 0.89Mb, 1.05Mb,1.36Mb, 2.05Mb, 2.20Mb, 2.80Mb, 3.05Mb, based on their migration relahve to the chromosomal DNAs ofSchizosacharomyces pombe and Saecharomyces cerevisiae, The genome sizes of strain NJ7 and NJ8 wereestimated to be 14. 02Mb and 13.87Mb respechvely. The results suggested that the molecular karyotype of stainNJ7 or NJ8 could be used as a new DNA size marker for molecular karyotype analysis of fungi.展开更多
蛋白分子量标记Amersham Life Science 建立了一组特别针对与 Amersham 的非放射性 ECL Western 点印系统一同使用而设计的蛋白质分子量标记。该标记为6种不同蛋白的混合物、为 SDS-PAGE 后在 Western 点印中提供了生物素化标签。与抗...蛋白分子量标记Amersham Life Science 建立了一组特别针对与 Amersham 的非放射性 ECL Western 点印系统一同使用而设计的蛋白质分子量标记。该标记为6种不同蛋白的混合物、为 SDS-PAGE 后在 Western 点印中提供了生物素化标签。与抗生蛋白链菌素辣根过氧化物酶温育后用 ECL 光检测,将在胶片上产型一组条带阶梯。定量检查证明此6种标准蛋白的分子量不因生物素化而明显改变。详情请与 Amersham Inter-展开更多
文摘The molecular karyotypes of strain NJ7 and NJS of Debaryomyces hansenii were obscened byusing pulsed-field gel electrophoresis (PFGE) which employs contour-clamped homogeneous electric field (CHEF).Both strains were with & chromosomal DNAs. The eshmated chromosomal DNA sizes of strain NJ7 were 0.47Mb,0.89Mb, 1.05Mb, 1.36Mb, 2.20Mb, 2.20Mb, 2.80Mb, 3.05Mb, and srain NJS were 0.47Mb, 0.89Mb, 1.05Mb,1.36Mb, 2.05Mb, 2.20Mb, 2.80Mb, 3.05Mb, based on their migration relahve to the chromosomal DNAs ofSchizosacharomyces pombe and Saecharomyces cerevisiae, The genome sizes of strain NJ7 and NJ8 wereestimated to be 14. 02Mb and 13.87Mb respechvely. The results suggested that the molecular karyotype of stainNJ7 or NJ8 could be used as a new DNA size marker for molecular karyotype analysis of fungi.